Optical trapping assisted detection rate enhancement of single molecules on a nanopore optofluidic chip

Rahman, M.; Harrington, M.; Stott, Matthew A.; Li, Y.; Sampad, Mohammad Julker Neyen; Yuzvinsky, T. D.; Hawkins, Aaron R.; Schmidt, Holger

doi:10.1364/optica.6.001130

Cited by 12 publications

(10 citation statements)

References 12 publications

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“…Therefore, nanopores mostly capture the analytes available within the very tiny capture volume, which in turn limits the nanopore's performance [14,[425][426][427]. To overcome this limitation, Rahman et al have demonstrated an elegant solution developed on the previously described ARROW optofluidic platform that can improve the performance of nanopore sensing by increasing the detection rate of analytes [422]. The core idea of their methodology is to accumulate the analytes and release them within the vicinity of the nanopore, aiming to increase the local concentration within the capture volume of the nanopore.…”

Section: Electrical Methods For Single-molecule Experimentsmentioning

confidence: 99%

A Critical Review on the Sensing, Control, and Manipulation of Single Molecules on Optofluidic Devices

Rahman

Islam

et al. 2022

Micromachines

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Single-molecule techniques have shifted the paradigm of biological measurements from ensemble measurements to probing individual molecules and propelled a rapid revolution in related fields. Compared to ensemble measurements of biomolecules, single-molecule techniques provide a breadth of information with a high spatial and temporal resolution at the molecular level. Usually, optical and electrical methods are two commonly employed methods for probing single molecules, and some platforms even offer the integration of these two methods such as optofluidics. The recent spark in technological advancement and the tremendous leap in fabrication techniques, microfluidics, and integrated optofluidics are paving the way toward low cost, chip-scale, portable, and point-of-care diagnostic and single-molecule analysis tools. This review provides the fundamentals and overview of commonly employed single-molecule methods including optical methods, electrical methods, force-based methods, combinatorial integrated methods, etc. In most single-molecule experiments, the ability to manipulate and exercise precise control over individual molecules plays a vital role, which sometimes defines the capabilities and limits of the operation. This review discusses different manipulation techniques including sorting and trapping individual particles. An insight into the control of single molecules is provided that mainly discusses the recent development of electrical control over single molecules. Overall, this review is designed to provide the fundamentals and recent advancements in different single-molecule techniques and their applications, with a special focus on the detection, manipulation, and control of single molecules on chip-scale devices.

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Section: Electrical Methods For Single-molecule Experimentsmentioning

confidence: 99%

A Critical Review on the Sensing, Control, and Manipulation of Single Molecules on Optofluidic Devices

Rahman

Islam

et al. 2022

Micromachines

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“…Then the potential energy of trapped GNPs in all the focal volume can be calculated by combining Eqs. 1and (3). Figure 2 shows the calculated results of the potential energy in the focal volume when the GNPs are trapped by linearly polarized fs laser beam in Gaussian mode.…”

Section: Theoretical Analysis and Numerical Simulationsmentioning

confidence: 99%

“…Optical tweezers have become a versatile tool that is widely applied in various research areas, especially in biophysical studies, materials science and soft condensed matter physics [1][2][3][4][5][6]. In the conventional optical tweezers, a continuous wave (CW) laser is usually used for light source, and the trapping is realized based on the linear interaction between sample and light.…”

Section: Introductionmentioning

confidence: 99%

Spheroidal trap shell beyond diffraction limit induced by nonlinear effects in femtosecond laser trapping

et al. 2020

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Beyond diffraction limit, multitrapping of nanoparticles is important in numerous scientific fields, including biophysics, materials science and quantum optics. Here, we demonstrate the 3-dimensional (3D) shell-like structure of optical trapping well induced by nonlinear optical effects in the femtosecond Gaussian beam trapping for the first time. Under the joint action of gradient force, scattering force and nonlinear trapping force, the gold nanoparticles can be stably trapped in some special positions, or hop between the trap positions along a route within the 3D shell. The separation between the trap positions can be adjusted by laser power and numerical aperture (NA) of the trapping objective lens. With a high NA lens, we achieved dual traps with less than 100 nm separation without utilizing complicated optical systems or any on-chip nanostructures. These curious findings will greatly extend and deepen our understanding of optical trapping based on nonlinear interaction and generate novel applications in various fields, such as microfabrication/nanofabrication, sensing and novel micromanipulations.

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“…However, the nanopore sensing paradigm poses its own set of challenges for creating a realistic diagnostic tool. The most prominent ones are ensuring specificity of the translocation signal for the desired target and efficient delivery of the targets to within the tiny (few micron) capture radius of the pore in which the electric field is strong enough to pull the targets through the opening ( Chuah et al, 2019 ; Rahman et al, 2019a ; Spitzberg et al, 2019 ). A few mitigation strategies such as concentration gradients and target delivery via magnetic nanoparticles have been demonstrated ( Chuah et al, 2019 ; Wanunu et al, 2010 ), but none have been translated to complete diagnostic assays.…”

Section: Introductionmentioning

confidence: 99%

“…In order to create an integrated, label-free, amplification-free nanopore sensor that is capable of analyzing patient samples at clinically relevant concentrations, we use a new approach called optical trapping-assisted capture rate enhancement (TACRE ( Rahman et al, 2019a )). It relies on a dual-beam optical trap of counterpropagating laser beams in liquid-core waveguides ( Kühn et al, 2009 ) to position target-carrying microbeads within the capture radius of a nanopore.…”

Section: Introductionmentioning

confidence: 99%

Optical trapping assisted label-free and amplification-free detection of SARS-CoV-2 RNAs with an optofluidic nanopore sensor

Sampad

Zhang

Yuzvinsky

et al. 2021

Biosensors and Bioelectronics

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Ultrasensitive, versatile sensors for molecular biomarkers are a critical component of disease diagnostics and personalized medicine as the COVID-19 pandemic has revealed in dramatic fashion. Integrated electrical nanopore sensors can fill this need via label-free, direct detection of individual biomolecules, but a fully functional device for clinical sample analysis has yet to be developed. Here, we report amplification-free detection of SARS-CoV-2 RNAs with single molecule sensitivity from clinical nasopharyngeal swab samples on an electro-optofluidic chip. The device relies on optically assisted delivery of target carrying microbeads to the nanopore for single RNA detection after release. A sensing rate enhancement of over 2,000x with favorable scaling towards lower concentrations is demonstrated. The combination of target specificity, chip-scale integration and rapid detection ensures the practicality of this approach for COVID-19 diagnosis over the entire clinically relevant concentration range from 10 4 -10 9 copies/mL.

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Optical trapping assisted detection rate enhancement of single molecules on a nanopore optofluidic chip

Cited by 12 publications

References 12 publications

A Critical Review on the Sensing, Control, and Manipulation of Single Molecules on Optofluidic Devices

A Critical Review on the Sensing, Control, and Manipulation of Single Molecules on Optofluidic Devices

Spheroidal trap shell beyond diffraction limit induced by nonlinear effects in femtosecond laser trapping

Optical trapping assisted label-free and amplification-free detection of SARS-CoV-2 RNAs with an optofluidic nanopore sensor

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