Optical trapping of red blood cells and two-photon excitation-based photodynamic study using a femtosecond laser

Fang-Lin, Mao; Xing, Qirong; Wang, Kai; Lang, Liying; Wang, Zhuan; Chai, Lu; Wang, Qingyue

doi:10.1016/j.optcom.2005.06.076

Cited by 39 publications

(15 citation statements)

References 15 publications

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“…In our numerical calculations, we simulate the radiation force produced by the incident focused FOGBs considered in one-dimension. We choose the peak intensity of the input beam as 11 2 0 3.10 W m I = with the above numerical parameters of λ and a . As well known, for a stable optical trapping, when a particle moves away, the radiation forces will pull the particle it back to the equilibrium position.…”

Section: Numerical Simulations and Results Discussionmentioning

confidence: 99%

“…This can absolutely help in handling micron particles. This topic was discussed in the literature first by Ashkin [6] and demonstrated how to manipulate three-dimensional trapping of a dielectric particle by using a highly focused laser beam thanks to their wide applications in manipulating various particles such as neutral atoms [7], molecules [8], micron-sized dielectric particles [9], DNA molecules [10] and living biological cells [11]- [13]. The optical traps or tweezers have also attracted attention in many literature works [14]- [18].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Radiation Forces on a Dielectric Sphere Produced by Finite Olver-Gaussian Beams

Hennani¹,

Ez-zariy²,

Belafhal³

2015

OPJ

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In this work, we use the analytical expression of the propagation of Finite Olver-Gaussian beams (FOGBs) through a paraxial ABCD optical system to study the action of radiation forces produced by highly focused FOGBs on a Rayleigh dielectric sphere. Our numerical results show that the FOGBs can be employed to trap and manipulate particles with the refractive index larger than that of the ambient. The radiation force distribution has been studied under different beam widths. The trapping stability under different conditions is also analyzed.

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Section: Numerical Simulations and Results Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Radiation Forces on a Dielectric Sphere Produced by Finite Olver-Gaussian Beams

Hennani¹,

Ez-zariy²,

Belafhal³

2015

OPJ

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“…The system used in this paper was described in detail by Mao et al [21] . A Ti: sapphire femtosecond laser operating at 810 nm was used.…”

Section: Femtosecond Laser Tweezersmentioning

confidence: 99%

“…Agate et al investigated the lateral trapping force (Q-value) of femtosecond optical tweezers on silica spheres [20] . Mao et al realized the trapping of biological cells using femtosecond laser and found that no evident damage on the target cells trapped for tens of minutes was found [21] , but they do not report further detailed study. One has reason to think about the potential damage to the biological particles by femtosecond laser tweezers, because direct evidence has shown that near-infrared CW laser light from optical tweezers can produce photodamage [22] or stress [23] in the trapped cells.…”

mentioning

confidence: 99%

Femtosecond optical trapping of cells: Efficiency and viability

Gong

Xing

2009

Trans. Tianjin Univ.

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Abstract：The femtosecond optical trapping capability and the effect of femtosecond laser pulses on cell viability were studied. The maximum lateral velocity at which the particles just failed to be trapped, together with the measured average trapping power, were used to calculate the lateral trapping force (Q-value). The viability of the cells after femtosecond laser trapping was ascertained by vital staining. Measurement of the Q-values shows that femtosecond optical tweezers are just as effective as continuous wave optical tweezers. The experiments demonstrate that there is a critical limit for exposure time at each corresponding laser power of femtosecond optical tweezers, and femtosecond laser tweezers are safe for optical trapping at low power with short exposure time.

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“…Recently, femtosecond laser has been theoretically and experimentally demonstrated being able to use for establishing femtosecond laser tweezers [10], [11].…”

Section: Introductionmentioning

confidence: 99%

Femtosecond Fiber Laser Applying for Cell Fusion

Nguyen

Mizuta

Taguchi

2015

Journal of the Japan Society of Applied Electromagnetics and Me

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We developed an actively mode-locked fiber laser that can generate 295 fs pulses at 9.188 MHz repetition rate. We built up a laser-induced cell fusion, in which the developed femtosecond laser was used as the laser source for both optical tweezers mode and laser scalpel mode, and thus improving cost-effectiveness. The cell fusion system also used a transparent dielectrophoresis chip as the specimen stage to create and manipulate the pearl chain of two or multiple cells for facilitating the cell fusion processes. We successfully developed the first optical tweezers using femtosecond fiber laser operating at 1530 nm, which can trap and transport cells effectively. With this developed system, we obtained the laser-induced fusion of red cabbage protoplasts. We also proposed a experimental cell fusion procedure which allows precisely selective cell fusion at the single-cell level. Therefore, the developed system would benefit basic research in biotechnology and biomedicine.

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Optical trapping of red blood cells and two-photon excitation-based photodynamic study using a femtosecond laser

Cited by 39 publications

References 15 publications

Radiation Forces on a Dielectric Sphere Produced by Finite Olver-Gaussian Beams

Radiation Forces on a Dielectric Sphere Produced by Finite Olver-Gaussian Beams

Femtosecond optical trapping of cells: Efficiency and viability

Femtosecond Fiber Laser Applying for Cell Fusion

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