Optical writing and single molecule reading of photoactivatable and silver nanoparticle-enhanced fluorescence

Dogantzis, Nicholas P.; Hodgson, Gregory K.; Impellizzeri, Stefania

doi:10.1039/d0na00049c

Cited by 17 publications

(74 citation statements)

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“…We recently reported a hybrid nanomolecular system for applications of fluorescence activation in solution and in thin polymer films . In brief, the highly quenched emission of boron dipyrromethene (BODIPY) species 1 can be turned on by optically stimulating a ligand exchange around the boron center using either UVA- or UVC-activated photoacid generators (PAGs) to form species 2 (Chart ).…”

Section: Introductionmentioning

confidence: 99%

“…We recently reported a hybrid nanomolecular system for applications of fluorescence activation in solution and in thin polymer films. 30 In brief, the highly quenched emission of boron dipyrromethene (BODIPY) species 1 can be turned on by optically stimulating a ligand exchange around the boron center using either UVA-or UVC-activated photoacid generators (PAGs) to form species 2 (Chart 1). We further established that triangular silver nanoparticles (AgNP) with a 104 ± 28 nm edge length (Figure S1) dramatically increase the steady-state fluorescence intensities of 1 and 2 through metalenhanced fluorescence (MEF) in thin films.…”

Section: ■ Introductionmentioning

confidence: 99%

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Single Molecule Techniques Can Distinguish the Photophysical Processes Governing Metal-Enhanced Fluorescence

2020

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Plasmonic metal nanoparticles can impact the behavior of organic molecules in a number of ways, including enhancing or quenching fluorescence. Only through a comprehensive understanding of the fundamental photophysical processes regulating nanomolecular interactions can these effects be controlled and exploited to the fullest extent possible. Metal-enhanced fluorescence (MEF) is governed by two underlying processes, increased rate of fluorophore excitation, and increased fluorophore emission, the balance between which has implications for optimizing hybrid nanoparticle–molecular systems for various applications. We report groundbreaking work on the use of single molecule fluorescence microscopy to distinguish between the two mechanistic components of MEF, in a model system consisting of two analogous boron dipyrromethene (BODIPY) fluorophores and triangular silver nanoparticles (AgNP). We demonstrate that the increased excitation MEF mechanism occurs to approximately the same extent for both dyes, but that the BODIPY with the higher quantum yield of fluorescence experiences a greater degree of MEF via the increased fluorophore emission mechanism and higher overall enhancement, as a result of its superior ability to undergo near-field interactions with AgNP. We foresee that this knowledge and methodology will be used to tailor MEF to meet the needs of different applications, such as those requiring maximum enhancement of fluorescence intensity or instead prioritizing excited-state photochemistry.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Single Molecule Techniques Can Distinguish the Photophysical Processes Governing Metal-Enhanced Fluorescence

2020

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“…1B, green traces). Previously, we demonstrated that the fluorescence of green-emitting probes based on the BODIPY platform can experience MEF by large triangular silver nanoplates (∼ 80 nm), for which the surface plasmon band is centred at 660 nm (Dogantzis et al, 2020; Golian et al, 2021; Hodgson et al, 2020). Indeed, large, non-spherical nanoparticles are known to exhibit increased scattering (Knoblauch et al, 2020; Lakowicz, 2005; Lakowicz et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

Improved imaging and preservation of lysosome dynamics using silver nanoparticle-enhanced fluorescence

Soha

Santhireswaran

Hodgson

et al. 2022

Preprint

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SummaryThe dynamics of living cells can be studied by live-cell fluorescence microscopy. However, this often requires the use of excessive light energy to obtain good signal-to-noise ratio, which can then photobleach the fluorochromes used, and more worrisome, lead to photo-toxicity. Thus, strategies that can reduce the amount and/or exposure to excitation light would improve live-cell microscopy. Upon light excitation, noble metal nanoparticles such as silver (AgNP) generate plasmons, which can amplify the excitation of fluorophores that are proximal to the surface of the nanoparticle. These can also couple to the oscillating dipole of nearby radiating fluorophores, which modifies the rate of emission and enhances their fluorescence. Here, we show that AgNP fed to cells to accumulate within lysosomes enhanced the fluorescence of lysosome-targeted BODIPY-cholesterol and DQ-BSA. Moreover, AgNP increased the fluorescence of GFP fused to the cytosolic tail of LAMP1, showing that metal enhanced fluorescence occurs across the lysosomal membrane. Importantly, by using AgNP, we could track lysosome motility with reduced laser power without damaging and altering lysosome dynamics. Overall, AgNP-enhanced fluorescence may be a useful tool to study the dynamics of the endo-lysosomal pathway while minimizing photo-toxicity.

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“…For example, optical writing and single-molecule reading of photoactivatable and silver nanoparticle enhanced fluorescence could be mentioned. 115 In this approach a hybrid nanoparticle-molecular system was developed, formed by silver nanoparticles and a fluorogenic boron dipyrromethene (BODIPY) that is selectively activated by UVA or UVC light in the presence of a targeted photoacid generator. In this manner accurate single-molecule readings were achieved in the near surrounding of the plasmonic structure.…”

Section: Perspectives Towards Biosensing and Single Molecule Detectio...mentioning

confidence: 99%

Development of nano- and microdevices for the next generation of biotechnology, wearables and miniaturized instrumentation

Palacios

Bracamonte

2022

RSC Adv.

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Optical writing and single molecule reading of photoactivatable and silver nanoparticle-enhanced fluorescence

Abstract: We present a hybrid nano-molecular system for optically activated, silver nanoparticle enhanced fluorescence in solution and in thin-polymer films, alongside single molecule level insights into the metal-enhanced fluorescence mechanism.

Cited by 17 publications

References 59 publications

Single Molecule Techniques Can Distinguish the Photophysical Processes Governing Metal-Enhanced Fluorescence

Single Molecule Techniques Can Distinguish the Photophysical Processes Governing Metal-Enhanced Fluorescence

Improved imaging and preservation of lysosome dynamics using silver nanoparticle-enhanced fluorescence

Development of nano- and microdevices for the next generation of biotechnology, wearables and miniaturized instrumentation

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