Optically controlled reversible protein hydrogels based on photoswitchable fluorescent protein Dronpa

Lyu, Shanshan; Fang, Jing; Duan, Tianyu; Fu, Linglan; Liu, Junqiu; Li, Hongbin

doi:10.1039/c7cc06991j

Cited by 70 publications

(77 citation statements)

References 37 publications

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“…In order to recapitulate these dynamic environments, several materials have been developed, which enable the reversible modulation of mechanical properties in response to chemical or optical stimuli. [3a–c,4] Since light as stimulus offers superior spatiotemporal control compared to classical chemical inducers, materials with reversibly adjustable mechanical properties based on sequential photodegradation and photoinitiated crosslinking, cis – trans isomerization of azobenzene, guest–host interaction of azobenzene and β‐cyclodextrin (all UV and violet light) as well as on the photoreceptors UVR8 (UV light), LOV2 (blue light), or Dronpa (violet and cyan light) were developed. However, materials that allow fast and fully reversible adjustment of mechanical properties under cell culture conditions with cell‐compatible and low energy red light are still lacking.…”

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confidence: 99%

Phytochrome‐Based Extracellular Matrix with Reversibly Tunable Mechanical Properties

et al. 2019

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The mechanical properties of the extracellular environment govern key cellular decision-making processes such as proliferation, differentiation, or migration. [1] Thus, analyzing how cells gauge and interact with their mechanical environment is critical not only for understanding physiological and pathological processes but also for engineering cell and tissue growth and differentiation in regenerative medicine. [2] Although studies using passive elastic or viscoelastic materials have revealed valuable information about cell-matrix interactions, matrices with adjustable mechanical properties more closely reflect the dynamic environments many cells are exposed to in a living organism. [3] In order to recapitulate these dynamic environments, several materials have been developed, which enable the Interrogation and control of cellular fate and function using optogenetics is providing revolutionary insights into biology. Optogenetic control of cells is achieved by coupling genetically encoded photoreceptors to cellular effectors and enables unprecedented spatiotemporal control of signaling processes. Here, a fast and reversibly switchable photoreceptor is used to tune the mechanical properties of polymer materials in a fully reversible, wavelengthspecific, and dose-and space-controlled manner. By integrating engineered cyanobacterial phytochrome 1 into a poly(ethylene glycol) matrix, hydrogel materials responsive to light in the cell-compatible red/far-red spectrum are synthesized. These materials are applied to study in human mesenchymal stem cells how different mechanosignaling pathways respond to changing mechanical environments and to control the migration of primary immune cells in 3D. This optogenetics-inspired matrix allows fundamental questions of how cells react to dynamic mechanical environments to be addressed. Further, remote control of such matrices can create new opportunities for tissue engineering or provide a basis for optically stimulated drug depots. BiomaterialsThe ORCID identification number(s) for the author(s) of this article can be found under https://doi.

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confidence: 99%

Phytochrome‐Based Extracellular Matrix with Reversibly Tunable Mechanical Properties

et al. 2019

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“…[74,80] This mechanism can be used in a variety of applications in the field of synthetic biology (Figure 2a). [74,80] This mechanism can be used in a variety of applications in the field of synthetic biology (Figure 2a).…”

Section: Chimeric and Customized Photosensitive Proteinsmentioning

confidence: 99%

Light‐Controlled Mammalian Cells and Their Therapeutic Applications in Synthetic Biology

2018

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The ability to remote control the expression of therapeutic genes in mammalian cells in order to treat disease is a central goal of synthetic biology‐inspired therapeutic strategies. Furthermore, optogenetics, a combination of light and genetic sciences, provides an unprecedented ability to use light for precise control of various cellular activities with high spatiotemporal resolution. Recent work to combine optogenetics and therapeutic synthetic biology has led to the engineering of light‐controllable designer cells, whose behavior can be regulated precisely and noninvasively. This Review focuses mainly on non‐neural optogenetic systems, which are often used in synthetic biology, and their applications in genetic programing of mammalian cells. Here, a brief overview of the optogenetic tool kit that is available to build light‐sensitive mammalian cells is provided. Then, recently developed strategies for the control of designer cells with specific biological functions are summarized. Recent translational applications of optogenetically engineered cells are also highlighted, ranging from in vitro basic research to in vivo light‐controlled gene therapy. Finally, current bottlenecks, possible solutions, and future prospects for optogenetics in synthetic biology are discussed.

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“…A similar approach was developed using CarH C , a protein that readily forms tetramers in the presence of adenosylcobalamin and is able to revert to monomers under green light (522 nm) . Apart from being used to release biological material from hydrogels, photoresponsive proteins, such as Dronpa145N, have been also investigated to reversibly form hydrogels . Due to its ability to form tetramers under cyan light (500 nm) and revert to monomers upon irradiation with violet light (400 nm), this versatile protein can even allow the formation of patterns on hydrogels via stereolithographic techniques .…”

Section: Hydrogel Scaffold Productionmentioning

confidence: 99%

The Role of Photochemical Reactions in the Development of Advanced Soft Materials for Biomedical Applications

Fernandez‐Villamarin

Brooks

Mendes

2019

Advanced Optical Materials

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In the biomedical field, many innovative materials to improve diagnosis and treatment of diseases are currently being developed. The ability to respond to different stimuli is one of the more interesting properties of such materials. Light, as one of the nature's elementary stimuli, offers an attractive and flexible stimulus for controlling the properties and functions of biomedical‐related materials. As such, photoresponsive systems have been increasingly pursued and finding applications in various biomedical settings, ranging from tissue engineering for the fabrication of bespoke tissue scaffolds to drug delivery, aims at manipulating treatment distribution inside the human body. Efforts have also been devoted to the development of highly efficient methods to control biological activity and bring us closer to mimicking impressive biological actuators. In this progress report, an overview of recent developments in the field is provided and current challenges discussed. Key strategies tailored to address specific issues are examined, offering useful perspectives for future designs.

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Optically controlled reversible protein hydrogels based on photoswitchable fluorescent protein Dronpa

Cited by 70 publications

References 37 publications

Phytochrome‐Based Extracellular Matrix with Reversibly Tunable Mechanical Properties

Phytochrome‐Based Extracellular Matrix with Reversibly Tunable Mechanical Properties

Light‐Controlled Mammalian Cells and Their Therapeutic Applications in Synthetic Biology

The Role of Photochemical Reactions in the Development of Advanced Soft Materials for Biomedical Applications

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