Optimal condition of cannabis maceration to obtain the high cannabidiol and Δ9-tetrahydrocannabinol content

Abstract

the aim of this work was to optimize a maceration condition of cannabis (Cannabis sativa l.). A circumscribed central composite experimental design was applied in this work. temperature and time were varied from 40-80 °C and 30-90 min, respectively. the three responses (i.e., extraction yield, cannabidiol content, and Δ 9-tetrahydrocannabinol content) were predicted by computer software. the yield was high when cannabis was macerated using ethanol at high temperature and long duration time. While cannabidiol and Δ 9-tetrahydrocannabinol content was high when macerating at a low heating temperature and short duration time. The optimal condition provided the simultaneous high of cannabidiol and Δ 9tetrahydrocannabinol content was 40 °C for 30 min. the prediction was accurate due to low percent error. this optimal condition could be used as a guide for maceration of cannabis to obtain the extract containing a high content of cannabidiol and Δ 9-tetrahydrocannabinol.

Cannabidiol from inflorescences of Cannabis sativa L.: Green extraction and purification processes

“…Many conventional and non-conventional solvent-based procedures have been already performed in the literature targeting cannabinoids from hemp. In this work the general, fast and common alcoholic solvent maceration method was selected in order to set a benchmark and compare results from supercritical methodologies (Fiorini et al, 2019;Monton et al, 2019;Mudge et al, 2017). By conventional methanolic extraction, the collected product was a green oil (shown in Fig.…”

Optimization of dynamic maceration of Clausena anisata (Willd.) Hook. f. ex Benth. leaves to maximize trans-anethole content

“…Our previous works demonstrated the extraction of plant active compounds from cannabis [36], Centella asiatica [37], and Vernonia cinerea [38]. All works applied circumscribed central composite experimental design, which has been widely used in response to surface modeling and optimization [39].…”

“…According to C. asiatica and cannabis, the active compounds were soluble in ethanol, so ethanol was selected as a safe extraction solvent. Due to the lower flash point and boiling point of ethanol compared with water, the lower extraction temperature was used: 40-60 °C for C. asiatica [37] and 40-80 °C for cannabis [36], and the longer extraction time could be applied: 60-120 min for C. asiatica [37] and 30-90 min for cannabis [36]. Our present work also used ethanol to extract trans-anethole, a volatile substance, so the low temperature range, 40-80 °C, and the short duration time, 10-30 min were selected.…”

Variability of cannabinoid yields of fibre hemp cultivars depending on the sowing density and nitrogen fertilisation

“…Cannabidiol (CBD) and Δ 9 -tetrahydrocannabinol (Δ 9 -THC) are hemp's most well-known active compounds. Δ 9 -THC is a primary psychoactive compound, while CBD is a non-psychoactive compound (Monton et al 2019, Poniatowska et al 2019. So far, the basic aspect of the legal regulations for the cultivation of hemp is the content of Δ 9 -THC, which by law cannot exceed 0.2% of dry weight (EU Regulation 2013).…”