OPTIMAL CONDITIONS FOR EXOPOLYSACCHARIDE PRODUCTION BY Lactobacillus plantarum T10

Abstract

Exopolysaccharide (EPS) production ability of Lactobacillus plantarumT10 was studied. The supplement of some sugars (lactose, saccharose, and glucose) gave the positive effects on EPS production of L. plantarum T10, in which the addition of lactose 4 % resulted in the most efficiency for EPS yield (274.83 µg/mL). The addition of 0.4 % of yeast extract into culture medium with 4 % lactose provided the highest EPS yields compared to other nitrogen sources (peptone, beef extract), which were 378.32 mg/mL. The optimal conditions for EPS production of L. plantarum T10 in MRS broth with 4 % of lactose and 0.4 % yeast extract supplement were also studied. The results indicated that the highest EPS yield (417.11 mg/L) was obtained in the conditions of 10 6 CFU/ml initial cell density, temperature of 35 o C, pH 5.5 and 48 h incubation.

Partial Purification, Characterization, and Application of Exopolysaccharides Produced by Lactobacillus plantarum NS1905E in Yogurt

“…Lactococcus, Streptococcus, Lactobacillus, Leuconostoc, and Pediococcus, often presented in raw milk, are associated with the manufacturing of dairy products. In the previous study, we have evaluated the EPS-producing abilities of LAB strains through the EPS content determination, and the isolated strain L. plantarum NS1905E (which was isolated from fresh milk in Nanshan Pasture, Nanshan Animal Husbandry Co., Ltd.) exhibited an excellent EPS-producing ability [14]. In the present study, we aim to characterize the partial purifed EPS produced by L. plantarum NS1905E, and its in vitro antioxidant activity and application in yogurt were evaluated.…”