Optimal Conditions for Production of Bacterial Amylase

El-Akher, M. Abd; El-Leithy, M.A.; El-Marsafy, M. K.; Kassim, S.A.

doi:10.1016/s0044-4057(73)80068-1

Cited by 3 publications

(1 citation statement)

References 6 publications

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“…Alpha amylase yield has been augmented from solid state fermented production media with proper supplementation of C and N sources as they are absolutely necessary for microbial growth [29,30]. From earlier literature it is known that the presence of (NH 4 ) 2 SO 4 , NH 4 Cl and NH 4 HPO 4 in SSF media along with WB stimulates enzyme production [31].…”

Section: Effect Of Extraction Solvent and Nitrogen Sources On The Amymentioning

confidence: 99%

Optimization of Solid State Fermentation Conditions and Characterization of Thermostable Alpha Amylase from Bacillus subtilis (ATCC 6633)

Mallik¹

2015

J Bioprocess Biotech

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Alpha amylase production using microbial source and solid state fermentation has been conducted for past few years in search of thermostable enzyme. Owing to the prolific use of thermostable alpha amylase in various industries like paper, food, detergent, brewing and starch liquefaction process, the production of alpha amylase is still going on. In the present work, Bacillus subtilis (ATCC 6633) has been utilized for generation of alpha amylase followed by optimization of the fermentation media. Change in fermentation conditions like fermentation hour, temperature, inoculums size, nitrogen and sugar sources have pivotal role to enhance alpha amylase yield. The thermal, pH and detergent stability of partially purified alpha amylase have been tested and compared with purified porcine pancreatic amylase. The result is encouraging with approximate 80% retention of alpha amylase activity comparable to purified porcine pancreatic amylase in presence of drastic condition of temperature (60°C), pH (6-11) and detergents. This makes it apt for use in various industries like detergent, food and paper industries.

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Section: Effect Of Extraction Solvent and Nitrogen Sources On The Amymentioning

confidence: 99%

Optimization of Solid State Fermentation Conditions and Characterization of Thermostable Alpha Amylase from Bacillus subtilis (ATCC 6633)

Mallik¹

2015

J Bioprocess Biotech

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A statistical approach for the production of thermostable and alklophilic alpha-amylase from Bacillus amyloliquefaciens KCP2 under solid-state fermentation

2014

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The bacterial strain producing thermostable, alklophilic alpha-amylase was identified as Bacillus amyloliquefaciens KCP2 using 16S rDNA gene sequencing data (NCBI Accession No: KF112071). Medium components were optimized through the statistical approach for the synthesis of alpha-amylase by the organism under solid-state fermentation using wheat bran as the substrate. The medium components influencing the enzyme production were identified using a two-level fractional factorial Plackett–Burman design. Among the various variables screened, starch, ammonium sulphate and calcium chloride were found to be most significant medium components. The optimum levels of these significant parameters were determined employing the response surface Central Composite design which significantly increased the enzyme production with the supplementation of starch 0.01 g, ammonium sulphate 0.2 g and 5 mM calcium chloride in the production medium. Temperature and pH stability of the alpha-amylase suggested its wide application in the food and pharmaceutical industries.

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