2009
DOI: 10.1007/s11814-009-0075-0
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Optimal culture condition for the production of phenyalanine ammonia lyase from E. coli

Abstract: The effects of culturing conditions on phenylalanine ammonia lyase production by a recombinant E. coli strain were investigated by using a controlled fed-batch fermentation system. In a 5 L fermentor, the optimal composition of the batch medium was 2% glucose, 1% yeast extract, 0.7% K 2 HPO 4 , 0.8% KH 2 PO 4 , 0.5% (NH 4 ) 2 SO 4 , 0.1% MgSO 4 ·7H 2 O. The optimal feed glucose solution was 50%. Glucose concentration and pH of the culture broth were maintained at about 2.0 g/L and 7.0 during the fed-batch phas… Show more

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Cited by 10 publications
(7 citation statements)
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“…The specific activity of PAL in E. coli JM109 (pBV-PAL) was higher than that reported by Faulkner et al (1994), it reached 35 U/g dried cell weight (DCW). PAL-specific activity in the recombinant E. coli (contain expression plasmid pBV-PAL) reached 300 U/g (DCW) by using a controlled fed-batch fermentation system in a 5 L jar fermentor (Cui & Li, 2009). However, the yield of recombinant PAL obtained still need to drastically increase for industrial and medical applications.…”
Section: Strategy Of Improving Pal Activitymentioning
confidence: 99%
“…The specific activity of PAL in E. coli JM109 (pBV-PAL) was higher than that reported by Faulkner et al (1994), it reached 35 U/g dried cell weight (DCW). PAL-specific activity in the recombinant E. coli (contain expression plasmid pBV-PAL) reached 300 U/g (DCW) by using a controlled fed-batch fermentation system in a 5 L jar fermentor (Cui & Li, 2009). However, the yield of recombinant PAL obtained still need to drastically increase for industrial and medical applications.…”
Section: Strategy Of Improving Pal Activitymentioning
confidence: 99%
“…Fed-batch culture under constant substrate feeding rate is a simple way to improve microbial biomass and product formation [18]. First, a high-cell-density culture of C. utilis SZU 07-01 was conducted by glucose feeding after 12 h under different constant feeding rate of 4.0, 5.0, 6.7 and 8.0 g/(L·h) until the total glucose concentration of 150 g/L to find if there existed a suitable glucose feeding rate (Fig.…”
Section: Fed-batch Glutathione Production Under Constant Glucose Feedmentioning
confidence: 99%
“…The harvested cells were suspended in the double distilled water (2%, w/w) as cells suspension [18].…”
Section: Preparation Of the Cell Suspensionmentioning
confidence: 99%