Optimal dosing time of Dachengqi decoction for protection of extrapancreatic organs in rats with experimental acute pancreatitis

Yao, Jiaqi; Zhu, Lin; Miao, Yifan; Zhu, Lv; Chen, Huan; Yuan, Ling; Hu, Jing; Yi, Xiao-Lin; Wu, Qiu-Ting; Yang, Xijing; Wan, Mei-Hua; Tang, Wen-Fu

doi:10.3748/wjg.v26.i22.3056

Cited by 4 publications

(8 citation statements)

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“…However, whether DCP affects Nrf2/HO-1/HMGB1 signaling in SAP remains unknown. DCQD has been proven to be one of the most commonly used Chinese herbal prescriptions with anti-inflammatory effects in the treatment of AP, and earlier studies by our team have also confirmed that DCQD could effectively alleviate multi-organ inflammatory responses in rats with SAP ( Zhao et al, 2015 ; Yao et al, 2020 ). Moreover, DCQD-derived compounds could reduce the levels of serum cTn1 and CK-MB, reduce the apoptosis of myocardial cells, and mitigate the pathological damage of pancreatic and cardiac tissues in rats with SAP ( Li et al, 2015 ).…”

Section: Discussionsupporting

confidence: 59%

“…We further explored the mechanism by which DCP regulated oxidative stress and inflammation. Da-Cheng-Qi decoction (DCQD) has been proved to be one of the most commonly used Chinese herbal prescriptions with anti-inflammatory effects on rats with SAP ( Zhao et al, 2015 ; Yao et al, 2020 ). Accordingly, the tissues from DCQD-treated rats were used as a control for further exploration of the mechanism (detailed information provided in Supplementary Material, Supplementary method).…”

Section: Resultsmentioning

confidence: 99%

“…Multiple groups were compared by oneway ANOVA followed by Dunnett's post-hoc test. All values are presented as mean ± SEM and statistical significance was set at p < 0.05. on rats with SAP (Zhao et al, 2015;Yao et al, 2020). Accordingly, the tissues from DCQD-treated rats were used as a control for further exploration of the mechanism (detailed information provided in Supplementary Material, Supplementary method).…”

Section: Discussionmentioning

confidence: 99%

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Dao-Chi Powder Ameliorates Pancreatitis-Induced Intestinal and Cardiac Injuries via Regulating the Nrf2-HO-1-HMGB1 Signaling Pathway in Rats

et al. 2022

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Dao-Chi powder (DCP) has been widely used in the treatment of inflammatory diseases in the clinical practice of traditional Chinese medicine, but has not been used in acute pancreatitis (AP). This study aimed to evaluate the effect of DCP on severe AP (SAP) and SAP-associated intestinal and cardiac injuries. To this end, an SAP animal model was established by retrograde injection of 3.5% taurocholic acid sodium salt into the biliopancreatic ducts of rats. Intragastric DCP (9.6 g/kg.BW) was administered 12 h after modeling. The pancreas, duodenum, colon, heart and blood samples were collected 36 h after the operation for histological and biochemical detection. The tissue distributions of the DCP components were determined and compared between the sham and the SAP groups. Moreover, molecular docking analysis was employed to investigate the interactions between the potential active components of DCP and its targets (Nrf2, HO-1, and HMGB1). Consequently, DCP treatment decreased the serum levels of amylase and the markers of gastrointestinal and cardiac injury, further alleviating the pathological damage in the pancreas, duodenum, colon, and heart of rats with SAP. Mechanistically, DCP rebalanced the pro-/anti-inflammatory cytokines and inhibited MPO activity and MDA levels in these tissues. Furthermore, Western blot and RT-PCR results showed that DCP intervention enhanced the expression of Nrf2 and HO-1 in the duodenum and colon of rats with SAP, while inhibiting the expression of HMGB1 in the duodenum and heart. HPLC-MS/MS analysis revealed that SAP promoted the distribution of ajugol and oleanolic acid to the duodenum, whereas it inhibited the distribution of liquiritigenin to the heart and ajugol to the colon. Molecular docking analysis confirmed that the six screened components of DCP had relatively good binding affinity with Nrf2, HO-1, and HMGB1. Among these, oleanolic acid had the highest affinity for HO-1. Altogether, DCP could alleviated SAP-induced intestinal and cardiac injuries via inhibiting the inflammatory responses and oxidative stress partially through regulating the Nrf2/HO-1/HMGB1 signaling pathway, thereby providing additional supportive evidence for the clinical treatment of SAP.

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Section: Discussionsupporting

confidence: 59%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Dao-Chi Powder Ameliorates Pancreatitis-Induced Intestinal and Cardiac Injuries via Regulating the Nrf2-HO-1-HMGB1 Signaling Pathway in Rats

et al. 2022

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“…The experimental program was approved by the Experimental Animal Ethics Review of Southwest Medical University (approval number: 20180517002). AP was induced following the protocol described by Yao et al (2020).…”

Section: Experimental Design and Samplingmentioning

confidence: 99%

Significant Succession of Intestinal Bacterial Community and Function During the Initial 72 Hours of Acute Pancreatitis in Rats

Wang

Luo

Qin

et al. 2022

Front. Cell. Infect. Microbiol.

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Acute pancreatitis (AP) is followed by structural and functional changes in the intestine, resulting from microbiome dysbiosis. However, it remains unclear how gut microbiome changes within the initial 72h of onset. In this study, severe acute pancreatitis (SAP), mild acute pancreatitis (MAP), and sham operation (SO) were replicated in rat models. 16S ribosomal RNA gene sequencing was used to explore the gut bacteria community. The predicted Cluster of Orthologous Genes (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathways were associated with the 16S rRNA profiles. Compared to the SO group, significant community succession was found during the initial 72h in AP group. At 72 h after AP induction, the Firmicutes/Bacteroidetes (F/B) ratios were significantly different, with the highest ratio in SAP group and the lowest in MAP group. Lactobacillus was the most abundant genus, but it nearly disappeared in SAP rats at 72 h. Clostridiaceae 1 and Clostridium sensu stricto 1 were significantly enriched in AP group. Bacteroidales S24-7 and Bacteroidales S24-7 group norank were enriched in MAP group, while Collinsella, Morganella, and Blautia were enriched in SAP group. Lactobacillus was significantly correlated with nine COGs. Nine COGs showed significant differences between AP group and SO group. Moreover, four COGs showed significant differences between the MAP and SAP groups. KEGG Level_3 pathways propanoate metabolism (Ko00640) in AP group was significantly higher than that in SO group. The aspartate‒ammonia ligase and four KEGG orthology terms of the AP group were lower than that in the SO group, respectively. All these results suggest that the intestinal bacterial community structure and function was changed during the initial 72h in AP rats. The intestinal F/B ratio and the relative abundance of Lactobacillus could be potential markers for early diagnosis of MAP and SAP. The genus Clostridium sensu stricto 1 was the most enriched genus in AP, and may be an important marker for AP.

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“…10 At present, the research on targeted pharmacodynamics mainly focuses on the pharmacodynamic substances (PS) or pharmacodynamic components; however, there are few reports on the interaction of pharmacodynamics efficacy. [11][12][13][14][15][16][17] This paper studies the explanatory power of pharmacodynamic substances (PS) toward the target pharmacodynamic indicators (TPI), and the explanatory power of pharmacodynamic indicators (PI) toward the target pharmacodynamic indicators (TPI). Specically, we focus on the pharmacodynamic interactions between the PIs.…”

Section: Introductionmentioning

confidence: 99%

Analysing pharmacodynamic interactions of traditional Chinese medicine in treating acute pancreatitis based on OPLS method

Nie,

Yu,

et al. 2024

Anal. Methods

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Optimal dosing time of Dachengqi decoction for protection of extrapancreatic organs in rats with experimental acute pancreatitis

Cited by 4 publications

References 49 publications

Dao-Chi Powder Ameliorates Pancreatitis-Induced Intestinal and Cardiac Injuries via Regulating the Nrf2-HO-1-HMGB1 Signaling Pathway in Rats

Dao-Chi Powder Ameliorates Pancreatitis-Induced Intestinal and Cardiac Injuries via Regulating the Nrf2-HO-1-HMGB1 Signaling Pathway in Rats

Significant Succession of Intestinal Bacterial Community and Function During the Initial 72 Hours of Acute Pancreatitis in Rats

Analysing pharmacodynamic interactions of traditional Chinese medicine in treating acute pancreatitis based on OPLS method

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