Optimal Formula of Angelica sinensis Ameliorates Memory Deficits in β-amyloid Protein-induced Alzheimer’s Disease Rat Model

Abstract

Objective Angelica (A.) sinensis is used as a traditional medical herb for the treatment of neurodegeneration, aging, and inflammation in Asia. A. sinensis optimal formula (AOF) is the best combination in A. sinensis that has been screened to rescue the cognitive ability in β-amyloid peptide (Aβ25–35)-treated Alzheimer’s disease (AD) rats. The objective of this study was to investigate the effect of AOF on the learning and memory of AD rats as well as to explore the underlying mechanisms. Methods Male Wistar rats were infused with Aβ25–35 for AD model induction or saline (negative control). Five groups of AD rats were fed on AOF at 20, 40, or 80 mL/kg every day, donepezil at 0.9 mg/kg every day (positive control), or an equal volume of water (AD model) intragastrically once a day for 4 weeks, while the negative control rats were fed on water. The Morris water maze test was used to evaluate the cognitive function of the rats. The Aβ accumulation, cholinergic levels, and antioxidative ability were detected by ELISA. Additionally, the candidate mechanism was determined by gene sequencing and quantitative real-time polymerase chain reaction. Results The results showed that AOF administration significantly ameliorated Aβ25–35-induced memory impairment. AOF decreased the levels of amyloid-β precursor protein and Aβ in the hippocampus, rescued the cholinergic levels, increased the activity of superoxide dismutase, and decreased the malondialdehyde level. In addition, AOF inhibited the expression of IL1b, Mpo, and Prkcg in the hippocampus. Conclusion These experimental findings illustrate that AOF prevents the decrease in cognitive function and Aβ deposits in Aβ25–35-treated rats via modulating neuroinflammation and oxidative stress, thus highlighting a potential therapeutic avenue to promote the co-administration of formulas that act on different nodes to maximize beneficial effects and minimize negative side effects.

Medicine–food herb:Angelica sinensis, a potential therapeutic hope for Alzheimer's disease and related complications

“…In addition, a mixture of polysaccharides, volatile oils and ferulic acid was found to decrease the levels of amyloid-β precursor protein and Aβ in the hippocampus, maintain the cholinergic levels, increase the activity of superoxide dismutase, and decrease the MDA level. 59 3.1.4 Compound recipes containing AS for treating AD. AS, as a frequently used medicine in Chinese, is often found in numerous herbal compound recipes.…”

“…In addition, a mixture of polysaccharides, volatile oils and ferulic acid was found to decrease the levels of amyloid-β precursor protein and Aβ in the hippocampus, maintain the cholinergic levels, increase the activity of superoxide dismutase, and decrease the MDA level. 59…”

East Wind, West Wind: Toward the modernization of traditional Chinese medicine

“…Angelica sinensis is an important component of both FSS and DGSYS, used for neurodegenerative diseases, as these preparations ameliorate memory impairment, decrease levels of both precursor and mature amyloid-β in the hippocampus, rescue cholinergic levels, increase activity of superoxide dismutase and decrease malondialdehyde levels. In addition, they inhibit the expression of inflammatory genes associated with Alzheimer’s disease such as IL1b (interleukin 1β) and Mpo (myeloperoxidase) in rat models of the disease ( Wang et al, 2022 ). However, in many cases, the mode of action and active compound/s in TCM are not known ( Table 1 ).…”