Optimal small kernels for edge detection

Reichenbach, S.E.; Park, S.K.; Alter-Gartenberg, Rachel

doi:10.1109/icpr.1990.119330

Cited by 8 publications

(2 citation statements)

References 11 publications

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“…Our work on removing these effects at low magnifications (6) should be continued at higher magnifications. Filter kernels other than those used in this study may be more effective in edge detection in that they also address sampling and optical blurring effects (17). The extreme linearity of the sensors should allow reliable measurement of the fluorescence of individual cells in all size ranges.…”

Section: Agesmentioning

confidence: 99%

Measurement of marine picoplankton cell size by using a cooled, charge-coupled device camera with image-analyzed fluorescence microscopy

Viles

Sieracki

1992

Appl Environ Microbiol

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Accurate measurement of the biomass and size distribution of picoplankton cells (0.2 to 2.0 ,um) is paramount in characterizing their contribution to the oceanic food web and global biogeochemical cycling. Image-analyzed fluorescence microscopy, usually based on video camera technology, allows detailed measurements of individual cells to be taken. The application of an imaging system employing a cooled, slow-scan charge-coupled device (CCD) camera to automated counting and sizing of individual picoplankton cells from natural marine samples is described. A slow-scan CCD-based camera was compared to a video camera and was superior for detecting and sizing very small, dim particles such as fluorochrome-stained bacteria. Several edge detection methods for accurately measuring picoplankton cells were evaluated. Standard fluorescent microspheres and a Sargasso Sea surface water picoplankton population were used in the evaluation. Global thresholding was inappropriate for these samples. Methods used previously in image analysis of nanoplankton cells (2 to 20 ,um) also did not work well with the smaller picoplankton cells. A method combining an edge detector and an adaptive edge strength operator worked best for rapidly generating accurate cell sizes. A complete sample analysis of more than 1,000 cells averages about 50 min and yields size, shape, and fluorescence data for each cell. With this system, the entire size range of picoplankton can be counted and measured.

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Section: Agesmentioning

confidence: 99%

Measurement of marine picoplankton cell size by using a cooled, charge-coupled device camera with image-analyzed fluorescence microscopy

Viles

Sieracki

1992

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…This reflects the fact that the restored value is used as a measure for the neighborhood rather than as point measure. Such partial restoration is a special case of the technique that [2] call characteristic restoration and can be implemented with a small kernel [16]. As these results indicate, more research into the use of restoration to enhance automated processing is needed.…”

Section: Discussionmentioning

confidence: 99%