Optimal support for heavily loaded columns in high speed liquid chromatography

Engelhardt, H.; Weigand, Norbert.

doi:10.1021/ac60329a009

Cited by 37 publications

(5 citation statements)

References 14 publications

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“…Apparently, the stationary liquid phase is strongly retained by the support; this unexpected result is difficult to explain because even if one assumes that the cooking procedure used may provoke chemical bonding between the solid surface and the molecules of the liquid phase, the number of• liquid molecules linked to the support can represent only an unsignificant fraction of the total amount of liquid phase contained within the pores. Although, therefore, we cannot furnish any definite explanation of this result, its practical implications are obvious: presaturation of the liquid carrier by stationary phase is superfluous in contrast with the techniques usually described in the literature (9,10, 32, 33).…”

Section: Spherosil In Liquid-liquid Chromatographymentioning

confidence: 72%

“…The composition in weight of the particle size distribution of this fraction was the following: >40 µ: 9%; [30][31][32][33][34][35][36][37][38][39][40] µ: 38%; 20-30 µ: 26%; <20 µ: 27%. Still smaller particles may be obtained either by crushing or by means of a special manufacturing process yielding perfectly spherical beads from 5 to 10 µ.…”

Section: Chromatographymentioning

confidence: 99%

“…Liquid carrier: heptane. ( )[30][31][32][33][34][35][36][37][38][39][40] µ: column length, 15 cm X 4 mm i.d. ( ) 10-40 µ; A. column length, 15 cm X 4 mm I.d.…”

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confidence: 99%

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Modern liquid chromatography on Spherosil

Vermont¹,

Deleuil²,

Vries³

et al. 1975

Anal. Chem.

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Section: Spherosil In Liquid-liquid Chromatographymentioning

confidence: 72%

Section: Chromatographymentioning

confidence: 99%

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Modern liquid chromatography on Spherosil

Vermont¹,

Deleuil²,

Vries³

et al. 1975

Anal. Chem.

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“…The liquid-liquid chromatography is a classic chromatographic separation mode with advantages of high sample capacity and easily changeable phase systems for diverse samples. 31,32 In this work, two arrays of picoliter-scale droplet stationary phase were formed on both sides of the microchannels using the droplet trapping technique previously developed in the authors' group. 21,22 The droplet array formation, sample injection and chromatographic separation were realized by coupling the chip with a slotted-vial array a Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou, 310058, China.…”

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confidence: 99%

Microfluidic droplet-array liquid–liquid chromatography based on droplet trapping technique

Zhu

Chen

et al. 2012

Lab Chip

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We describe the first realization of liquid chromatographic separation in a droplet-based microfluidic system and develop a novel mode for microchip-based chromatography named as droplet-array liquid-liquid chromatography. In this system, two arrays of picoliter-scale droplets immobilized on both sidewalls of a microchannel with droplet trapping technique served as the stationary phase in chromatographic separation, while the other immiscible phase flowing in the microchannel served as the mobile phase. The chromatographic separation was achieved on the basis of multiple extraction and elution of analytes between the droplet array stationary phase and the mobile phase. The proof-of-concept study of the droplet-array LC system was performed in the separation of fluoranthene and benzo[b]fluoranthene. Under the optimum conditions, the two analytes were separated within 26 min with separation efficiencies of 112 μm and 119 μm plate height, respectively. The advantages of the present system include simple structure, low driving pressure, and relatively high sample capacity. It can also provide a useful platform for LC theory study and educational purposes by allowing the researchers and students to directly "see" the continuous extraction and elution process of a chromatographic separation.

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“…The total peak broadening of polystyrene samples on a porous silica column was measured using "dry" dichloromethane as eluent. If the column is equilibrated with water-saturated dichloromethane ("wet" eluent), the total pore volume is filled with water, producing a heavily loaded column (41)(42)(43)(44). Since the pore volume is now inaccessible to polystyrene samples, it is possible to measure their interstitial band broadening.…”

mentioning

confidence: 99%