Optimasi Suhu dan Waktu Deasetilasi Kitin Berbasis Selongsong Maggot (Hermetia ilucens) Menjadi Kitosan

Abstract

As Black Soldier Fly farming has become an emerging trend in Indonesia there is a new opportunity to take advantage from the waste generated from the activity. One of them is the exuviae, which is resulted from the emergence of the fly and it has a high chitin content. Chitin from BSF exuviae have low Cristalinity Indexes. Hence, it shows a promising potential to be processed to produce a new biopolimer. Therefore, converting chitin from BSF exuviae into chitosan using deacetylation process was studied. The researched variables were temperature and the duration of the process, which were studied at 50, 60, 70, and 80°C for 12 hours and 8, 10, 12, and 14 hours at 60°C, respectively. Chitosan produced in the temperature of 60, 70, and 80°C has met the standard quality, and the best duration of deacetylation process was 8 hours. The solubility test in acetic acid (2%) showed that chitosan solubility was increased with the increase of temperature, while it decreased as the duration of the process becomes longer. Moreover, the degree of deacetylation showed that chitosan was produced with the best quality in 80°C of temperature (75.98%) and 8 hours of deacetylation process (71.12%).
 
 Keywords: chitosan, degrees of deacetylation, exuviae of black soldier fly

Analisis Produksi Biodiesel dan Kitosan Berbasis Larva Black Soldier Fly (Hermetia Illucens) dengan Memanfaatkan Sampah Organik

“…Proses deproteinasi dilakukan dengan cara merendam pupa BSF dalam larutan NaOH 2M selama 36 jam. Proses tersebut akan mengeliminasi protein karena ion Na + akan mengikat ujung protein yang bermuatan negatif sehingga larut dalam NaOH (Wahyuni et al, 2020).…”

“…Rendemen kitin yang dihasilkan dari proses-proses tersebut yaitu sebesar 11,8%. Rendemen kitin yang diperoleh sangat bergantung dengan fase hidup dari BSF yang digunakan (Wahyuni et al, 2020).…”

“…Derajat deasetilasi kitosan pada suhu 80 C diperoleh sebesar 75,98% (Gambar 2b). Angka tersebut telah memenuhi standar derajat deasetilasi kitosan yang ditetapkan Soleh BSN yakni di atas 75% (Wahyuni et al, 2020).…”

“…Gambar 2 Rendemen kitosan berdasarkan optimasi a) variasi suhu, b) variasi waktu (Sumber: (Wahyuni et al, 2020).…”

Peningkatan kadar capsaicin tanaman Capsicum annuum cv. Lado pada kondisi kekeringan menggunakan kitosan

“…Kitosan merupakan senyawa alami turunan dari kitin, polisakarida yang terdapat pada eksoskeleton kelompok crustaceae seperti udang, lobster, kepiting, dan juga dinding sel jamur maupun selongsong pupa black soldier fly (BSF) (Mondal et al, 2012;Pichyangkura & Chadchawan, 2015;Wahyuni et al, 2020). Kitosan dan derivatnya bersifat tidak beracun dan mudah terdegradasi oleh mikroba tanah, sehingga bersifat ramah lingkungan.…”