Optimierter Radioextraktionsassay zur quantitativen Bestimmung der Aktivität von Diaminooxidase (DAO) in humanem Serum und Plasma

Mayer, I.; Missbichler, Albert; Wantke, F.; Focke, Manfred; Reichl, H.; Winter, Martin; Jarisch, R.

doi:10.5414/alp28001

Cited by 12 publications

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“…It may be complemented by an analysis of DAO and HMT gene polymorphisms to identify a possible genetic predisposition. We measured DAO by enzyme immunoassay analysis in serum samples . For evaluation, we used the manufacturer's recommended reference values, and all our patients had highly reduced activity of DAO <40 HDU/ml.…”

Section: Casementioning

confidence: 99%

Idiopathic Anaphylaxis and Histamine Intolerance

Ivković‐Jureković

2015

Pediatric Allergy Immunology

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Section: Casementioning

confidence: 99%

Idiopathic Anaphylaxis and Histamine Intolerance

Ivković‐Jureković

2015

Pediatric Allergy Immunology

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“…Activity of the resulting enzyme-antibody complex was determined with the radioextraction assay from SCIOTEC [24]. No decrease of activity was found up to an antibody concentration of 1 mg/ml.…”

Section: Measurement Of Dao Activity After Binding On Igymentioning

confidence: 99%

“…HIC fractions with the highest activity of DAO were further purified on a QAE-column. DAO activity was quantified by radioextraction assay as described by Mayer et al [24]. The last purification step was done on a cellulose sulfate column.…”

Section: Purification Of Diamine Oxidase From Porcine Kidneymentioning

confidence: 99%

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Highly Sensitive Determination of DAO Activity by Oxidation of a Luminescence Reagent

Mayer

Pittner

Hermann

et al. 2007

Appl Biochem Biotechnol

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A highly sensitive method for measuring the activity of the enzyme diamine oxidase (DAO) independent of the type of substrate is described. The principle of the assay is to determine the amount hydrogen peroxide generated as a reaction product during oxidation of diamines by DAO. PSatto, a highly sensitive luminescence reagent, was used to generate a signal depending on the hydrogen peroxide concentration based on the action of horseradish peroxidase. DAO is specifically captured from a sample by an antibody immobilized to microwell plates, and the substrate is added to the bound enzyme. Various diamines were used as substrates; the peroxide produced is directly proportional to the amount of DAO bound to the specific antibodies. With this very sensitive method, it is possible to detect pmol amounts of generated hydrogen peroxide in plasma matrix corresponding to the biological activity of DAO.

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“…In brief, an assay for DAO activity is based on the conversion of [1, 4- 14 C]putrescine to 4-amino-[1, 4- 14 C] butylaldehyde which in alkaline solution spontaneously forms Δ1-[1, 4- 14 C]pyrroline that can be extracted into an organic solvent for quantization [6]. This assay has been widely used for diagnosis of histamine intolerance and diminished DAO levels in plasma imputed to histamine intolerance [6, 7]. However, this remained un successful as these results could not be confirmed by others [8, 9].…”

Section: Introductionmentioning

confidence: 99%

Histamine 50-Skin-Prick Test: A Tool to Diagnose Histamine Intolerance

Kofler¹,

Ulmer

Kofler³

2011

ISRN Allergy

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Background. Histamine intolerance results from an imbalance between histamine intake and degradation. In healthy persons, dietary histamine can be sufficiently metabolized by amine oxidases, whereas persons with low amine oxidase activity are at risk of histamine toxicity. Diamine oxidase (DAO) is the key enzyme in degradation. Histamine elicits a wide range of effects. Histamine intolerance displays symptoms, such as rhinitis, headache, gastrointestinal symptoms, palpitations, urticaria and pruritus. Objective. Diagnosis of histamine intolerance until now is based on case history; neither a validated questionnaire nor a routine test is available. It was the aim of this trial to evaluate the usefullness of a prick-test for the diagnosis of histamine intolerance. Methods. Prick-testing with 1% histamine solution and wheal size-measurement to assess the relation between the wheal in prick-test, read after 20 to 50 minutes, as sign of slowed histamine degradation as well as history and symptoms of histamine intolerance. Results. Besides a pretest with 17 patients with HIT we investigated 156 persons (81 with HIT, 75 controls): 64 out of 81 with histamine intolerance(HIT), but only 14 out of 75 persons from the control-group presented with a histamine wheal ≥3 mm after 50 minutes (P < .0001). Conclusion and Clinical Relevance. Histamine-50 skin-prickt-test offers a simple tool with relevance.

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Optimierter Radioextraktionsassay zur quantitativen Bestimmung der Aktivität von Diaminooxidase (DAO) in humanem Serum und Plasma

Cited by 12 publications

References 0 publications

Idiopathic Anaphylaxis and Histamine Intolerance

Idiopathic Anaphylaxis and Histamine Intolerance

Highly Sensitive Determination of DAO Activity by Oxidation of a Luminescence Reagent

Histamine 50-Skin-Prick Test: A Tool to Diagnose Histamine Intolerance

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