Optimisation and characterisation of silica-based reversed-phase liquid chromatographic systems for the analysis of basic pharmaceuticals

Vervoort, R.J.M.; Debets, A.J.J.; Claessens, H.A.; Cramers, C.A.M.G.; Jong, Gerhardus J. de

doi:10.1016/s0021-9673(00)00811-6

Cited by 128 publications

(56 citation statements)

References 90 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Rapid analysis methods are beneficial for high throughput; however, results may vary depending on the separation technique selected. Current techniques for profiling, characterization and analysis of glycans include hydrophilic 70 liquid chromatography (HILIC), 4-7 reverse phase chromatography (RP), 8,9 capillary electrophoresis (CE), [10][11][12] mass spectrometry (MS), [13][14][15][16][17] nuclear magnetic resonance (NMR) 18,19 amongst others.…”

Section: Introductionmentioning

confidence: 99%

Comparison of separation techniques for the elucidation of IgG N-glycans pooled from healthy mammalian species

Adamczyk

Tharmalingam-Jaikaran

Schomberg

et al. 2014

Carbohydrate Research

View full text Add to dashboard Cite

a b s t r a c tThe IgG N-glycome provides sufficient complexity and information content to serve as an excellent source for biomarker discovery in mammalian health. Since oligosaccharides play a significant role in many biological processes it is very important to understand their structure. The glycosylation is cell type specific as well as highly variable depending on the species producing the IgG. We evaluated the variation of N-linked glycosylation of human, bovine, ovine, equine, canine and feline IgG using three orthogonal glycan separation techniques: hydrophilic interaction liquid chromatography (HILIC)-UPLC, reversed phase (RP)-UPLC and capillary electrophoresis with laser induced fluorescence detection (CE-LIF). The separation of the glycans by these high resolution methods yielded different profiles due to diverse chemistries. However, the % abundance of structures obtained by CE-LIF and HILIC-UPLC were similar, whereas the analysis by RP-UPLC was difficult to compare as the structures were separated by classes of glycans (highly mannosylated, fucosylated, bisected, fucosylated and bisected) resulting in the co-elution of many structures. The IgGs from various species were selected due to the complexity and variation in their N-glycan composition thereby highlighting the complementarity of these separation techniques.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparison of separation techniques for the elucidation of IgG N-glycans pooled from healthy mammalian species

Adamczyk

Tharmalingam-Jaikaran

Schomberg

et al. 2014

Carbohydrate Research

View full text Add to dashboard Cite

show abstract

“…Currently, the most commonly used analytical technique in the pharmaceutical industry is HPLC [12,[56][57][58][59]. Reversed-phase HPLC (RP-HPLC) in particular, has proven to be very versatile in its ability to simultaneously analyze small molecular weight compounds of different polarities [60][61][62][63].…”

Section: Determination Of Basic Drugs By Hplcmentioning

confidence: 99%

Characterization of pharmaceutical drugs by a modified nonaqueous capillary electrophoresis – mass spectrometry method

et al. 2003

View full text Add to dashboard Cite

A simple method for the separation and characterization of a group of nine basic compounds, comprising seven tricyclic antidepressant and two bronchodilator drugs, by nonaqueous capillary electrophoresis (NACE) employing ultraviolet and mass spectrometry detection is described. After optimization of the electrophoresis separation conditions, including the compositions of the electrolyte and the organic solvent, a reliable separation of all nine basic analytes was achieved in 80 mM ammonium formate dissolved in a methanol-acetonitrite (80:20 v/v) mixture, having an apparent pH of 8.7. The volatile nonaqueous electrolyte system used with a normal electroosmotic flow polarity also provided an optimal separation condition for the characterization of the analytes by mass spectrometry. When results were compared with reversed-phase gradient and isocratic high-performance liquid chromatography (HPLC) methods, the NACE method provided greater efficiency, achieving baseline resolution for all nine basic compounds in less than 30 min. The NACE method is suitable for use as a routine procedure for the rapid separation and characterization of basic compounds and is a viable alternative to HPLC for the separation of a wide range of pharmaceutical drugs.

show abstract

“…Due to the use of polar endcapping, polar embedding (modifying alkyl chains with polar functional group) or mixed ligands, these alternative phases provide additional retention mechanisms that often result in increased retention of polar compounds and different selectivity compared to more traditional alkyl-bonded phases [3][4][5][6]. Unfortunately, the information about bond-ing technology and nature of functional groups used for polar endcapping or embedding is usually classified as proprietary by manufacturers, which makes it difficult to predict the applicability of these novel phases for chromatographic determination of highly polar multifunctional compounds.…”

Section: Introductionmentioning

confidence: 99%

Applicability of alkyl‐bonded ultra‐pure silica stationary phases for gradient reversed‐phase HPLC of folates with conventional and volatile buffers under highly aqueous conditions

Patring¹,

Lanina²,

Jastrebova³

2006

J of Separation Science

View full text Add to dashboard Cite

Applicability of several alkyl-bonded silica stationary phases was tested for gradient RP-HPLC of folates under highly aqueous conditions. High retention of folates was achieved on alternative phases with enhanced polarity and classical phases with higher carbon content. Phases exhibiting polar secondary interactions were found to provide better selectivity for late-eluting folates, whereas selectivity for early-eluting folates was mostly dependent on hydrophobic interactions. Best selectivity in phosphate buffered mobile phase was achieved on polar-endcapped silica phases (Aquasil C18 and HyPurity Aquastar) followed by alternative Atlantis dC18. Classical phases exhibited poorer separation of 10-formyl-folic acid and 5-formyl-tetrahydrofolate, but it could be considerably improved by increasing the buffer pH. Strong secondary interactions of ion-exchange character on polar-embedded phases resulted in marked peak deterioration, loss of recovery and dramatic changes in retention behaviour for early- and late-eluting folates when changing the mobile phase composition and pH. Therefore, polar-embedded phases such as HyPurity Advance were found to be unsuitable for separating folates. Stationary phases exhibited peak deterioration when using volatile buffer of low ionic strength. Better results were obtained with classical phases, whereas alternative phases showed not only peak deterioration but also a decrease in recovery and poorer selectivity due to increased secondary interactions in volatile buffer.

show abstract

Optimisation and characterisation of silica-based reversed-phase liquid chromatographic systems for the analysis of basic pharmaceuticals

Cited by 128 publications

References 90 publications

Comparison of separation techniques for the elucidation of IgG N-glycans pooled from healthy mammalian species

Comparison of separation techniques for the elucidation of IgG N-glycans pooled from healthy mammalian species

Characterization of pharmaceutical drugs by a modified nonaqueous capillary electrophoresis – mass spectrometry method

Applicability of alkyl‐bonded ultra‐pure silica stationary phases for gradient reversed‐phase HPLC of folates with conventional and volatile buffers under highly aqueous conditions

Contact Info

Product

Resources

About