Optimisation of ethanol fermentation of Jerusalem artichoke tuber juice using simple technology for a decentralised and sustainable ethanol production

Matías, Javier; Martín, Juan Vicente González; González, Jerónimo; González, J.F.

doi:10.1016/j.esd.2014.12.009

Cited by 32 publications

(15 citation statements)

References 24 publications

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“…This plant is considered as a promising candidate for consolidated bioprocessing (CBP), which enables the utilization of whole plant biomass (the tuber and stalk). Jerusalem artichoke tuber, with a high content of inulin, has been investigated as a sugar source for bioethanol production [ 31 ]. As for Jerusalem artichoke stalk, there is no report about using its lignocellulosic materials for fungal growth and induction of lignocellulolytic enzymes.…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of the secretomes of Schizophyllum commune and other wood-decay basidiomycetes during solid-state fermentation reveals its unique lignocellulose-degrading enzyme system

Zhu

Liu

Yang

et al. 2016

Biotechnol Biofuels

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BackgroundThe genome of Schizophyllum commune encodes a diverse repertoire of degradative enzymes for plant cell wall breakdown. Recent comparative genomics study suggests that this wood decayer likely has a mode of biodegradation distinct from the well-established white-rot/brown-rot models. However, much about the extracellular enzyme system secreted by S. commune during lignocellulose deconstruction remains unknown and the underlying mechanism is poorly understood. In this study, extracellular proteins of S. commune colonizing Jerusalem artichoke stalk were analyzed and compared with those of two white-rot fungi Phanerochaete chrysosporium and Ceriporiopsis subvermispora and a brown-rot fungus Gloeophyllum trabeum.ResultsUnder solid-state fermentation (SSF) conditions, S. commune displayed considerably higher levels of hydrolytic enzyme activities in comparison with those of P. chrysosporium, C. subvermispora and G. trabeum. During biodegradation process, this fungus modified the lignin polymer in a way which was consistent with a hydroxyl radical attack, similar to that of G. trabeum. The crude enzyme cocktail derived from S. commune demonstrated superior performance over a commercial enzyme preparation from Trichoderma longibrachiatum in the hydrolysis of pretreated lignocellulosic biomass at low enzyme loadings. Secretomic analysis revealed that compared with three other fungi, this species produced a higher diversity of carbohydrate-degrading enzymes, especially hemicellulases and pectinases acting on polysaccharide backbones and side chains, and a larger set of enzymes potentially supporting the generation of hydroxyl radicals. In addition, multiple non-hydrolytic proteins implicated in enhancing polysaccharide accessibility were identified in the S. commune secretome, including lytic polysaccharide monooxygenases (LPMOs) and expansin-like proteins.ConclusionsPlant lignocellulose degradation by S. commune involves a hydroxyl radical-mediated mechanism for lignocellulose modification in parallel with the synergistic system of various polysaccharide-degrading enzymes. Furthermore, the complex enzyme system of S. commune holds significant potential for application in biomass saccharification. These discoveries will help unveil the diversity of natural lignocellulose-degrading mechanisms, and advance the design of more efficient enzyme mixtures for the deconstruction of lignocellulosic feedstocks.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0461-x) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Comparative analysis of the secretomes of Schizophyllum commune and other wood-decay basidiomycetes during solid-state fermentation reveals its unique lignocellulose-degrading enzyme system

Zhu

Liu

Yang

et al. 2016

Biotechnol Biofuels

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“…Energies 2018, 11 10 of 17 reactions of commonly found inhibitors may have been present in the medium [57]. Luo et al [58] found 2-furancarboxylic acid, 2-furanacetic acid, and 5-hydroxymethylfurancarboxylic acid, of which furfural and HMF are precursors, in nitric-acid hydrolysate of aspen chips.…”

Section: Concentration Of Inhibitors and Fermentation Of Hydrolysatesmentioning

confidence: 99%

Nitric Acid Pretreatment of Jerusalem Artichoke Stalks for Enzymatic Saccharification and Bioethanol Production

et al. 2018

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This paper evaluated the effectiveness of nitric acid pretreatment on the hydrolysis and subsequent fermentation of Jerusalem artichoke stalks (JAS). Jerusalem artichoke is considered a potential candidate for producing bioethanol due to its low soil and climate requirements, and high biomass yield. However, its stalks have a complexed lignocellulosic structure, so appropriate pretreatment is necessary prior to enzymatic hydrolysis, to enhance the amount of sugar that can be obtained. Nitric acid is a promising catalyst for the pretreatment of lignocellulosic biomass due to the high efficiency with which it removes hemicelluloses. Nitric acid was found to be the most effective catalyst of JAS biomass. A higher concentration of glucose and ethanol was achieved after hydrolysis and fermentation of 5% (w/v) HNO3-pretreated JAS, leading to 38.5 g/L of glucose after saccharification, which corresponds to 89% of theoretical enzymatic hydrolysis yield, and 9.5 g/L of ethanol. However, after fermentation there was still a significant amount of glucose in the medium. In comparison to more commonly used acids (H2SO4 and HCl) and alkalis (NaOH and KOH), glucose yield (% of theoretical yield) was approximately 47–74% higher with HNO3. The fermentation of 5% nitric-acid pretreated hydrolysates with the absence of solid residues, led to an increase in ethanol yield by almost 30%, reaching 77–82% of theoretical yield.

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“…Finally, the glucose is fermented into ethanol by 3 of 25 yeast cells [11,12]. In the process to convert starch into ethanol, starch granules must be gelatinized and liquefied at high temperature before saccharification and fermentation [13]. The conventional enzymatic liquefaction and saccharification of starch has many disadvantages.…”

Section: Introductionmentioning

confidence: 99%

Single-Step Ethanol Production from Raw Cassava Starch Using a Combination of Raw Starch Hydrolysis and Fermentation, Scale-Up from 5-L Laboratory and 200-L Pilot Plant to 3,000-L Industrial Fermenters

Krajang

Malairuang

Sukna

et al. 2020

Preprint

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Background: A single-step ethanol production is the combination of raw cassava starch hydrolysis and fermentation. For the development of raw starch consolidated bioprocessing (CBP) technologies, this research work was to investigate the optimum conditions and technical procedures for the production of ethanol from raw cassava starch in a single step. This resulted high yields and productivities of all the experiments from the laboratory, the pilot, through the industrial scales. The yields of ethanol concentration are comparable with those in the commercial industries that use molasses and hydrolyzed starch as the raw materials. Results: Before single-step ethanol production, the studies of raw cassava starch hydrolysis by a granular starch hydrolyzing enzyme, StargenTM002, were carefully conducted. It successfully converted 80.19% (w/v) of raw cassava starch to glucose at a concentration of 176.41 g/L with a productivity of 2.45 g/L/h when the raw starch was pretreated at 60 °C for 1 h with 0.10% (v/w dry starch basis) of Distillase ASP before hydrolysis. A single-step ethanol production at 34 °C in a 5-L fermenter showed that S. cerevisiae (Fali, active dry yeast) produced the maximum ethanol concentration, p of 81.86 g/L (10.43% v/v) with a yield coefficient, Y p/s of 0.41 g/g, a productivity or production rate, r p of 1.14 g/L/h with an efficiency, Ef of 71.44%. The scale-up experiments of the single-step ethanol production using this method, from the 5-L fermenter to the 200-L fermenter and further to the 3,000-L industrial fermenter were successfully achieved with essentially good results. The p, Y p/s , r p , and Ef values of the 200-L scale were 80.85 g/L (10.23% v/v), 0.41 g/g, 1.12 g/L/h and 72.47% , respectively ; of the 3,000-L scale were 70.74 g/L (9.01% v/v), 0.34 g/g, 0.98 g/L/h and 59.82% , respectively. Because of using raw starch, the major by-products of all the three scales were very low; glycerol lactic acid and acetic acid, in ranges of 0.94-1.14%, 0.046-0.052%, 0-0.059% (w/v), respectively, where are less than those values in the industries. Conclusions: This single-step ethanol production using a combination of raw cassava starch hydrolysis and fermentation of the three fermentation scales here is practicable and feasible for the scale-up of industrial production of ethanol from raw starch.

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Optimisation of ethanol fermentation of Jerusalem artichoke tuber juice using simple technology for a decentralised and sustainable ethanol production

Cited by 32 publications

References 24 publications

Comparative analysis of the secretomes of Schizophyllum commune and other wood-decay basidiomycetes during solid-state fermentation reveals its unique lignocellulose-degrading enzyme system

Comparative analysis of the secretomes of Schizophyllum commune and other wood-decay basidiomycetes during solid-state fermentation reveals its unique lignocellulose-degrading enzyme system

Nitric Acid Pretreatment of Jerusalem Artichoke Stalks for Enzymatic Saccharification and Bioethanol Production

Single-Step Ethanol Production from Raw Cassava Starch Using a Combination of Raw Starch Hydrolysis and Fermentation, Scale-Up from 5-L Laboratory and 200-L Pilot Plant to 3,000-L Industrial Fermenters

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