Optimisation of submerged culture conditions for the production of mycelial biomass and exopolysaccharide byPleurotus nebrodensis

Liu, Jia; Hu, Shunzhen; Xu, Mei

doi:10.1007/bf03175078

Cited by 17 publications

(5 citation statements)

References 2 publications

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“…Hence, rotary speed at 160 rpm was desirable for both mycelial growth and EPS production when the solid seed was used. This result is generally in accordance with the findings of several previous reports, where the optimum for EPS production by G. lucidum was 150 rpm (Yang and Liau, 1998) and 160 rpm by Pleurotus nebrodensis (Le et al, 2007).…”

Section: Effects Of Rotary Speedsupporting

confidence: 93%

“…Inoculum size significantly influencing mycelial growth and EPS production in submerged fermentation of mushrooms has been well documented Le et al, 2007). The effect of inoculum size of the soybean meal solid seed on mycelial growth and EPS production was investigated by holding rotary speed at 160 rpm and temperature at 28°C, with natural initial pH (around 6.0).…”

Section: Effects Of Inoculum Sizementioning

confidence: 99%

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Optimization of submerged culture conditions involving a developed fine powder solid seed for exopolysaccharide production by the medicinal mushroom Ganoderma lucidum

Liu

Zhang

2018

Food Sci Biotechnol

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To facilitate Ganoderma lucidum submerged cultivation and achieve high productivity, four fine powder solid substrates incorporated with different nitrogen-rich supplements were utilized to grow the fungus and as solid seed for its submerged culture. Of the four solid seeds, the soybean meal solid seed gave the highest biomass (10.73 g/ L) and exopolysaccharide (EPS) (1.22 g/L), higher than those (8.36 g/L biomass and 0.44 g/L EPS) obtained with mycelial liquid seed. The optimal level of soybean meal supplementation was 20% (w/w) for production of the solid seed. Following single factor experiments, levels of three selected process variables were optimized as: the moisture content of solid seed, 70%; inoculum size, 0.8 g/flask; and rotary speed, 160 rpm. These conditions were validated experimentally with improved EPS yield of 1.33 g/L. The developed solid seed can be conveniently used for G. lucidum submerged culture with improved EPS productivity.

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Section: Effects Of Rotary Speedsupporting

confidence: 93%

Section: Effects Of Inoculum Sizementioning

confidence: 99%

Optimization of submerged culture conditions involving a developed fine powder solid seed for exopolysaccharide production by the medicinal mushroom Ganoderma lucidum

Liu

Zhang

2018

Food Sci Biotechnol

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“…The maximal EPS production was obtained when maltose was used as the carbon source (Table 1). Similar patterns have been observed in culture studies of various edible mushrooms, such as Ganoderma lucidum (Tang and Zhong, 2002), Pleurotus nebrodensis (Le et al, 2007), Agrocybe cylindracea (Kim et al, 2005). It was indicated in Table 1 that maltose is favorable for EPS production, but not so favorable for cell growth.…”

Section: Effects Of Carbon Sourcesupporting

confidence: 75%

Polysaccharides production by submerged fermentation of Coprinus comatus and their inhibitory effects on non-enzymatic glycosylation

Ding¹

2012

J. Med. Plants Res.

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Bioactive polysaccharides produced by fungi, especially by medicinal mushrooms, have attracted great interest. Polysaccharides from Coprinus comatus have been reported to possess hypoglycemic activity. In the present study, the effects of carbon source, initial glucose concentration and initial peptone level on cell growth and polysaccharide production were examined in the submerged fermentation system of C. comatus. Maltose was found to be disadvantageous for cell growth, but favorable for extracellular polysaccharide (EPS) production. Glucose was beneficial for both cell growth and production of intracellular polysaccharide (IPS), which was markedly improved when a high initial glucose concentration was used. The highest maximal EPS production level (0.89 g/l) was obtained with an initial glucose concentration of 40 g/l. Cell growth was positively related to increases in initial peptone concentration and polysaccharide production was maximized at 10 g/l of peptone. Finally, high inhibitory activity of Coprinus polysaccharides (both EPS and IPS) on non-enzymatic glycosylation (NEG) was demonstrated for the first time. The findings in this study can be applied to improve the performance of C. comatus cultures in the production of bioactive metabolites on a bioreactor scale and provide a foundation for further investigation into medicinally active compounds derived from C. comatus.

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“…Although some research has been carried out in solid media, the use of liquid medium (submerged culture) is preferred due to its advantages in terms of growth and ease of handling, although Dudekula et al (2020) point out that this system has its own drawbacks that must be overcome in each case. Le et al (2007) worked on optimizing the growth of Pleurotus nebrodensis to obtain exopolysaccharides, using maltose as carbon source and yeast extract as nitrogen source, and obtained 4.13 g L -1 of biomass. Elisashvili (2012), with glucose as carbon source, obtained biomass yields (g L -1 ) of 5.6 (Lentinus edodes), 7.7 (Agaricus nevoi) and 5.3 -11.3 (three species of Pleurotus) in submerged culture.…”

Section: Edible Mushrooms Biotechnology Edible Mushrooms Biotechnolog...mentioning

confidence: 99%

Edible mushrooms – perspectives and considerations

PÉREZ-ÁLVAREZ,

HECTOR-ARDISANA,

RASCÓN SOLANO

et al. 2023

Not Sci Biol

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The cultivation of edible mushrooms has been an activity of great ecological, social and economic importance for many years. There is a great diversity of different species of these mushrooms, truffles, chanterelles, pine mushrooms, Termitomyces, with more than 1100 registered species, many of which are of great economic importance not only for export but also for developing countries. The collection and consumption of these mushrooms vary by country, being, for example, extensive and intensive in China and more restricted in South American countries. The ecological value they have should also be highlighted, since some species live in symbiosis with trees, helping the growth of forests and commercial plantations. These fungi contribute to human nutrition supplying micronutrients, polysaccharides, amino acids, fibers and proteins; also, they synthesize active secondary metabolites such as saponins, glycosides, alkaloids, flavonoids, tannins, polyphenols and some other reducing compounds. This review about edible mushrooms addresses the generalities and production, description of several species, some toxic species in Mexico and finally several aspects of these mushrooms’ biotechnology.

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Optimisation of submerged culture conditions for the production of mycelial biomass and exopolysaccharide byPleurotus nebrodensis

Cited by 17 publications

References 2 publications

Optimization of submerged culture conditions involving a developed fine powder solid seed for exopolysaccharide production by the medicinal mushroom Ganoderma lucidum

Optimization of submerged culture conditions involving a developed fine powder solid seed for exopolysaccharide production by the medicinal mushroom Ganoderma lucidum

Polysaccharides production by submerged fermentation of Coprinus comatus and their inhibitory effects on non-enzymatic glycosylation

Edible mushrooms – perspectives and considerations

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