Optimisation of ultrasound-assisted extraction of phenolic antioxidants from Ilex guayusa Loes. leaves using response surface methodology

Crespo, Yasiel Arteaga; Radice, Matteo; Sánchez, Luis Ramón Bravo; Quintana, Yudel García; Scalvenzi, Laura

doi:10.1016/j.heliyon.2019.e03043

Cited by 38 publications

(30 citation statements)

References 51 publications

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“…The sample tube with the mixture was placed in a Trans-sonic T460 ultrasonic bath (Elma, Singen, Germany). The operative conditions for the extraction were optimized through response surface methodology (RSM) [ 16 ]. A four factor Box-Behnken design was defined to investigate the effects of parameters such as time, temperature, power and solid/liquid ratio on water UAE of industrial hemp inflorescences.…”

Section: Methodsmentioning

confidence: 99%

Metabolomic Profile and Antioxidant/Anti-Inflammatory Effects of Industrial Hemp Water Extract in Fibroblasts, Keratinocytes and Isolated Mouse Skin Specimens

et al. 2021

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Industrial hemp is a multiuse crop whose phytocomplex includes terpenophenolics and flavonoids. In the present study, the phenolic and terpenophenolic compounds were assayed in the water extract of the hemp variety Futura 75. Protective effects were also investigated in human fibroblast and keratinocytes and isolate mouse skin specimens, which were exposed to hydrogen peroxide and/or to the extract (1–500 µg/mL). The results of phytochemical analysis suggested the cannabidiol, cannabidiolic acid and rutin as the prominent phytocompounds. In the in vitro system represented by human keratinocytes and fibroblasts, the hemp extract was found to be able to protect cells from cytotoxicity and apoptosis induced by oxidative stress. Moreover, modulatory effects on IL-6, a key mediator in skin proliferation, were found. In isolated rat skin, the extract reduced hydrogen peroxide-induced l-dopa turnover, prostaglandin-E2 production and the ratio kynurenine/tryptpophan, thus corroborating anti-inflammatory/antioxidant effects. The in silico docking studies also highlighted the putative interactions between cannabidiol, cannabidiolic acid and rutin with tyrosinase and indoleamine-2,3-dioxygenase, involved in l-dopa turnover and tryptophan conversion in kynurenine, respectively. In conclusion, the present findings showed the efficacy of hemp water extract as a skin protective agent. This could be partly related to the extract content in cannabidiol, cannabidiolic acid and rutin.

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Section: Methodsmentioning

confidence: 99%

Metabolomic Profile and Antioxidant/Anti-Inflammatory Effects of Industrial Hemp Water Extract in Fibroblasts, Keratinocytes and Isolated Mouse Skin Specimens

et al. 2021

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“…In the present study, temperature of 60°C promoted better extraction of TPC and SS. This result was also observed in the UAE of active compounds (He et al., 2016, Zhang, Tang, Zheng, Li, & Pan, 2019, Machado et al., 2019, Arteaga‐Crespo, Radice, Bravo‐Sanchez, García‐Quintana, & Scalvenzi, 2020) and soluble sugars (Ramón et al., 2015).…”

Section: Resultsmentioning

confidence: 55%

Ultrasound‐assisted extraction of phenolic compounds and soluble sugars from the stem portion of peach palm

Donadone

Giombelli²,

Silva³

et al. 2020

J Food Process Preserv

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The aim of this work was to investigate the ultrasound‐assisted extraction (UAE) of total phenolic compounds (TPC) and soluble sugars (SS) from the by‐product of peach palm (stem portion). The effect of the processing variables temperature (30–60°C), time (15–45 min), and power density (8.47–25.42 mW cm−3) were evaluated. The maximum contents (dry basis) of TPC (345.81 ± 2.82 mg gallic acid equivalent 100 g−1) and SS (16.68 ± 0.24 g 100 g−1) were obtained, respectively, at 60°C, 30 min and 8.47 mW cm−3 (condition 1) and 60°C, 45 min and 25.42 mW cm−3; in these conditions, the extracts had higher sucrose content than glucose and fructose. The highest content of phenolic acids (gallic, coumaric, vanillic, ferulic, trans‐cinnamic) and highest antioxidant activity were obtained in the condition 1. UAE can be considered an effective method for the extraction of TPC and SS from peach palm stem. Practical applications The ultrasound‐assisted extraction (UAE) was applied in the by‐product of heart‐of‐palm (stem portion) to the removal of phenolic compounds and soluble sugars. To realize this process with time and energy economy, the maximized condition of extraction observed at 60°C, 30 min, and 8.47 mW cm−3 was the most interesting. This extract showed the highest content of phenolic compounds and highest antioxidant potential, which can allow its application to preserve the shelf life of food products or to produce food supplements. The soluble sugars extracted can also be used, as example, in fermentative process. Therefore, the results indicate the viability of UAE to offer alternatives to utilization of stem portion of peach palm.

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“…The two-dimensional (2D) contour and threedimensional (3D) surface plots are used to graphically represent the interactive effects of the studied variables with respect to the responses 34 . The responses plots of quercitrin content were presented in Figure 2 (a-c), while Figure 3 (a-b) shows the response plots of total phenolic content.…”

Section: Analysis Of the Contour And Surface Plotmentioning

confidence: 99%

“…Various types of sample matrix can be applied in the Box-Behnken experimental design for extraction of the phenolic compounds such as samara oil 32 , algae 31 , glutinous rice bran 33 , cherry fruits 24 , Ilex guayusa Loes. leaves 34 and Citrus medica 16 .…”

Section: Introductionmentioning

confidence: 99%

Ultrasonic-Assisted Extraction (UAE) for Enhanced Recovery of Bioactive Phenolic Compounds From Cosmos Caudatus Leaves

Latiff

Ong

Abdullah

et al. 2021

Preprint

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Cosmos caudatus (C. caudatus) is one of the common medicinal plants and among the valuable plants that are high in bioactive compounds such as phenolics. In this study, an ultrasound-assisted extraction (UAE) method was used to optimise the extraction of bioactive compounds from C. caudatus leaves using response surface methodology (RSM) and employing a Box-Behnken experimental design (BBD). The extraction efficiency of UAE under the optimal extraction conditions was compared with the Soxhlet method. Also, an anti-microbial analysis against two human pathogenic bacteria; Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) was also evaluated. The effect of the extraction conditions was studied and optimised such as the solid-liquid ratio (10 to 30 g/ml), particle size (180 to 850 µm) and extraction time (20 to 30 min). Quercitrin and total phenolic content (TPC) were the selected response variables in this study. Based on the ANOVA analysis, the response surface model to predict the optimum yield of quercitrin and TPC was adequate with a high R-square value corresponding to 0.9930 and 0.9962. The optimal UAE conditions were 1:28 (g/mL), by using a particle size of 485 µm and an extraction time of 30 min, respectively. Remarkably, UAE reduced the extraction time and solvent volume, with the maximal recovery of bioactive compounds at a high antioxidant activity as compared with that of the Soxhlet method. The plant extract also exhibited potential microbial agents. Due to the above findings, UAE can be used to enrich quercitrin and total phenolic content from C. caudatus leaves. It also opens the possibility of plant extract to be used as an affordable component in many applications such as food formulations and anti-microbial agents.

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Optimisation of ultrasound-assisted extraction of phenolic antioxidants from Ilex guayusa Loes. leaves using response surface methodology

Cited by 38 publications

References 51 publications

Metabolomic Profile and Antioxidant/Anti-Inflammatory Effects of Industrial Hemp Water Extract in Fibroblasts, Keratinocytes and Isolated Mouse Skin Specimens

Metabolomic Profile and Antioxidant/Anti-Inflammatory Effects of Industrial Hemp Water Extract in Fibroblasts, Keratinocytes and Isolated Mouse Skin Specimens

Ultrasound‐assisted extraction of phenolic compounds and soluble sugars from the stem portion of peach palm

Ultrasonic-Assisted Extraction (UAE) for Enhanced Recovery of Bioactive Phenolic Compounds From Cosmos Caudatus Leaves

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