Optimisation studies on neomycin production by a mutant strain of Streptomyces marinensis in solid state fermentation

Ellaiah, P; Srinivasulu, B; Adinarayana, K

doi:10.1016/s0032-9592(02)00059-6

Cited by 99 publications

(54 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The ranges of the variables were selected based on prior biological knowledge as followed: many fungi show broad pH optima from 5.5 to 7.5, but the enzymes needed in many natural environments often have specific pH optima and function over a quite narrow pH range (6). Therefore, the pH of 6.0 was selected.…”

Section: Resultsmentioning

confidence: 99%

The potential of an Aspergillus fumigatus Brazilian strain to produce antimicrobial secondary metabolites

Furtado

Fonseca

Bastos

2005

Braz. J. Microbiol.

View full text Add to dashboard Cite

During the course of screening for biologically leading active natural products from fungi, a strain of Aspergillus fumigatus was assessed for antimicrobial activity production. Four parameters consisting of medium culture, pH, temperature and time of fermentation were investigated. The extracts from the fungus cultures displayed different TLC profiles and produced, at least, one inhibition zone in the bioautography assays. Overlapping inhibition zones in the range of Rf 0.09 to 0.65 against Kocuria rhizophila were detected in the bioautography assay of the chloroform extract obtained from Jackson's medium culture, initial pH of 6.0 and incubation at 40°C for 144 hours. However, the obtained extracts by developing the fungus culture in Vogel's medium furnished the smallest number of active compounds. The obtained chromatographic profiles of the extracts from the fungus cultures by high performance liquid chromatography with photodiode array detector were distinctive, allowing to infer that A. fumigatus secondary metabolites production could be affected by modifying any of the undertaken parameters. It should be pointed out that a remarkable difference among HPLC profiles was observed by using different media culture.

show abstract

Section: Resultsmentioning

confidence: 99%

The potential of an Aspergillus fumigatus Brazilian strain to produce antimicrobial secondary metabolites

Furtado

Fonseca

Bastos

2005

Braz. J. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Genus Streptomyces is one of the most famous actinomycete genera with beneficial roles inenzymes and antibiotics production (Ellaiah et al, 2004;Aly et al, 2011b). The knowledge on the participation and role of actinomycetes in hydrolysis of PHB are extremely limited and need to be elucidated in further studies.…”

Section: Discussionmentioning

confidence: 99%

Poly-𝛽-hydroxy butyrate Depolymerase from Streptomyces lydicus MM10, Isolated from Wastewater Sample

Aly¹,

Tork²,

Qari³

et al. 2015

IJAB

View full text Add to dashboard Cite

To cite this paper: Aly, M.M., S. AbstractPlastics are an integral part in our daily life activities and it is difficult to conceive how we could function without them. Burning or chemical treatments of these plastic materials destroys the environment and have many dangerous effects on, water air and human health. Thus, other sources of plastic (biodegradable plastic) must be introduced. Under unfavorable growth conditions Poly ß-hydroxybutyrate (PHB) is accumulated intracellular by many bacteria and the principal enzyme for the degradation of PHB is PHB depolymerase. A number of thermophilic actinomycetes have been screened for PHB degradation on agar plats. All these isolates were recovered from soil, sand and wastewater samples on starch nitrate agar at incubation temperature of 45. Five isolates (50%) out of 10 showed degradation activities, detected by the diameter of the clear zone. The Actinomycete isolate MM10 was the most PHB degrader and it was identified using morphological, physiological and biochemical characters. According to 16SrRNA analysis, it was identified as Streptomyces lydicusMM10. Growth at 45ᵒC, in minimal medium (pH7.0) containing glucose and0.3% PHB as enzyme inducer, enhanced enzyme production and maximum activity was obtained after 24 h of incubation. The PHB depolymerase was precipitated and purified using column chromatography. The optimum temperature and pH of the pure enzyme were 45°C and pH 8, respectively. However, production of bioplastics is cost effective as compared to synthetic plastics they were non-toxic and completely biodegradable by some actinomycete isolates

show abstract

“…Similarly, the optimum temperature for antimicrobial metabolite production was determined by incubating the strain at temperatures ranging from 20 to 40°C, while maintaining all other conditions at optimum levels [10]. The effect of carbon sources on bioactive metabolite production was determined by supplementing the production medium (YMD) with different carbon sources such as maltose, sucrose, mannitol, lactose, starch, cellulose, galactose, sorbitol, and fructose each at a concentration of 0.4% (w/v) replacing dextrose by keeping the other ingredients constant [11]. Influence of varying concentrations of the best carbon source (0.5-4% w/v) on bioactive metabolite production was also investigated.…”

Section: Media Optimizationmentioning

confidence: 99%

Optimization, Isolation and Characterization of Bioactive Compounds From Streptomyces Lavendulocolor VHB-9

Bssn

Munaganti

Vijayalakshmi

et al. 2018

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Objectives: Optimization, isolation, and characterization of bioactive compounds from Streptomyces lavendulocolor VHB-9 isolated from granite mines of Mudigonda village of Khammam district of Telangana state. Methods:The potent strain was identified as S. lavendulocolor VHB-9 by polyphasic taxonomy. The influence of culture conditions on growth and bioactive compounds production was investigated. Purification of bioactive compounds was done using column chromatography. The structures of the compounds were elucidated on the basis of spectroscopic analysis including Fourier transform infrared, electron spray ionization mass spectrophotometry, 1 H nuclear magnetic resonance (NMR), and 13 C NMR. The antimicrobial activity of the compounds produced by the strain was tested against both Gram-positive and Gram-negative bacteria and fungi in terms of minimum inhibitory concentration.Results: Isolation and identification of two compounds, namely (2R, 3R)-2, 3-Butanediol (B1A), and nonadecanoic acid (B1B). Fraction B4 was isolated partially purified fraction and identified by the gas chromatography-mass spectrometry analysis. B1B compound exhibited the highest activity against Bacillus megaterium, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans when compared to B1A and B4 compounds.

show abstract

Optimisation studies on neomycin production by a mutant strain of Streptomyces marinensis in solid state fermentation

Cited by 99 publications

References 26 publications

The potential of an Aspergillus fumigatus Brazilian strain to produce antimicrobial secondary metabolites

The potential of an Aspergillus fumigatus Brazilian strain to produce antimicrobial secondary metabolites

Poly-𝛽-hydroxy butyrate Depolymerase from Streptomyces lydicus MM10, Isolated from Wastewater Sample

Optimization, Isolation and Characterization of Bioactive Compounds From Streptomyces Lavendulocolor VHB-9

Contact Info

Product

Resources

About