The potential of an Aspergillus fumigatus Brazilian strain to produce antimicrobial secondary metabolites

Abstract

During the course of screening for biologically leading active natural products from fungi, a strain of Aspergillus fumigatus was assessed for antimicrobial activity production. Four parameters consisting of medium culture, pH, temperature and time of fermentation were investigated. The extracts from the fungus cultures displayed different TLC profiles and produced, at least, one inhibition zone in the bioautography assays. Overlapping inhibition zones in the range of Rf 0.09 to 0.65 against Kocuria rhizophila were detected in the bioautography assay of the chloroform extract obtained from Jackson's medium culture, initial pH of 6.0 and incubation at 40°C for 144 hours. However, the obtained extracts by developing the fungus culture in Vogel's medium furnished the smallest number of active compounds. The obtained chromatographic profiles of the extracts from the fungus cultures by high performance liquid chromatography with photodiode array detector were distinctive, allowing to infer that A. fumigatus secondary metabolites production could be affected by modifying any of the undertaken parameters. It should be pointed out that a remarkable difference among HPLC profiles was observed by using different media culture.

FUNGOS ALERGÊNICOS E TOXIGÊNICOS NO COMPOSTO DE CULTIVO DE Agaricus brasiliensis

“…Penicillium funiculosum 1 ND (3) Penicillium oxalicum 3 1 Considering the results obtained in this work, it is recommended that workers involved in the production and compost management wear masks to protect them against Aspergillus fumigatus and other allergenic fungi.…”

“…For each sample, 20 g of compost were weighed, which were mixed with 180 cm 3 4 , 0.4 g; glycerol 95%, 185 cm 3 ; dichloran 0.1%, 1 cm 3 ; chloramphenicol, 10 mg; agar, 15 g). The plates were incubated at 25 ºC for 7 days.…”

“…Therefore, the cotton waste-based substrate did favor an abnormal production of A. fumigatus spores; however, it is necessary to note that their presence may be common in any kind of compost, since this species widely occurs in nature and plays an important role in recycling organic matter (Furtado et al, 2005).…”

Antimicrobial properties of sclerotiorin, isocHromophilone VI and pencolide, metabolites from a Brazilian cerrado isolate of Penicillium sclerotiorum van Beyma

“…Fungi have proved to be capable of biosynthesizing secondary metabolites bearing conspicuous structural diversity, which could be further enlarged by structure modification. Among 20 of the most commonly prescribed medications, six are from fungal origin (2).…”

Isolation, purification and characterization of novel antimicrobial compound 7-methoxy-2,2-dimethyl-4-octa-4′,6′-dienyl-2H-napthalene-1-one from Penicillium sp. and its cytotoxicity studies

“…The wonder drug directed the interest of the researchers towards natural resources having different biological activities. Since then, a number of compounds with antibiotic activity have been isolated from different fungi such as, Penicillium janczewskii, Penicillium canescens (Kozlovskii et al 1997 ), Penicillium sclerotiorium, Penicillium janthinellum, Penicillium citrinum (Takahashi et al 2008 ), Myrothecium cinctum (Kobayashi et al 2004 ) and Aspergillus fumigatus (Furtado et al 2005 ), etc. Fungi are historically important sources of secondary metabolites and they continue to provide new chemical entities with novel biological activities (Baker and Alvi 2004 ).…”