Optimised production and utilisation of exopolysaccharide from Agrobacterium radiobacter

Triveni, R.; Shamala, T. R.; Rastogi, N. K.

doi:10.1016/s0032-9592(00)00279-x

Cited by 110 publications

(66 citation statements)

References 11 publications

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“…The fitted polynomial equation is expressed as surface and contour plots in order to visualize the relationship between the response and experimental levels of each factor and to deduce the optimum conditions (Triveni et al, 2001). The analysis of variance tables were generated and the effect and regression coefficients of individual linear, quadratic and interaction terms were determined.…”

Section: Resultsmentioning

confidence: 99%

Optimization of Extraction Process of Crude Protein from Grape Seeds by RSM

Jia

et al. 2011

FSTR

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Grape seed is a potential source of edible protein, so effective extraction of protein component from grape seed seems to be very important. However, so far, such information is unavailable. In this study, the conditions for protein extraction from the grape seeds were optimized by the single factor test and the response surface methodology (RSM). A box-behnken design (BBD) was used for experimental design and analysis of the results to obtain the optimal extraction conditions. Solvent/meal ratio, extraction temperature, pH and extraction time were found to have a significant effect on the protein yield. From the single factor test, the optimal range of extraction conditions was obtained. Based on the RSM analysis, optimum extraction parameters were obtained as following: solvent/meal ratio 22.5/1 (v/w), extraction temperature 35℃, pH 9.8, and extraction time 29 min. Under the optimized conditions, the experimental values were in good agreement with those predicted by the model. These results help design the process of optimal protein extraction from grape seeds for future use in food industry.

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Section: Resultsmentioning

confidence: 99%

Optimization of Extraction Process of Crude Protein from Grape Seeds by RSM

Jia

et al. 2011

FSTR

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“…Moreover, there has been no research about degrading chitosan with NaClO alone. Response surface methodology is a statistical method that is useful for mathematical modeling and simultaneously solving multivariate equations (Triveni et al 2001). It can quantify the correlation between the independent input parameters and the dependent response (Aslan and Cebeci 2007).…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Low Molecular Weight Chitosan

Yuan¹,

Jiang²,

Xing³

et al. 2015

BioResources

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Chitosan can be widely used in many areas owing to its unique properties, although its poor solubility in water is still a limiting factor. In the present study, low molecular weight chitosan (LMWC) was prepared by degradation with NaClO so that chitosan was able to dissolve in water. Chitosan to liquor ratio, NaClO content, temperature, and time were considered variables of NaClO degradation, and the Box-Behnken design was used to determine optimal conditions. There was good agreement between the experimental data and their predicted counterparts. The optimum conditions for chitosan degradation were estimated to be 1:67.91 of chitosan to liquor ratio, 22.03% of NaClO content, a temperature of 90.3 °C, and a time of 3.07 h. It was found that synthesis under these optimized conditions achieved the lowest molecular weight (10,937.4 Daltons). In addition, Fourier transform infrared spectroscopy, X-ray diffractograms, and thermogravimetric analysis showed that the structure of LMWC was similar to the original chitosan, while the crystallinity and thermal stability decreased after degradation.

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“…The media optimization for higher PHA production by traditional method is tedious and time consuming. Hence response surface methodology (RSM) has been used for optimization of different process parameters in a single experiment (Triveni et al, 2001). The chapter deals with optimization of media for PHA production by the selected Bacillus sp, extraction and characterization of the polymer produced by the bacterium.…”

Section: Discussionmentioning

confidence: 99%

“…As reported earlier (Triveni et al, 2001) a central composite rotatable design (CCRD) with five variables, at five levels (citric acid, KH 2 PO 4 , glucose, (NH 4 ) 2 HPO 4 and inoculum) was used to study the response pattern and optimum combination of the variables used (Table 37). the product CCRD was arranged to fit the regression model using multiple regression program (Table 40).…”

Section: Experimental Designmentioning

confidence: 99%

Symbiosis Among Lixus scrobicollis (Coleoptera: Curculionidae), Ambrosia trifida, and Bacillus endophyticus

Gans

Malaer

Padilla

2014

Southwestern Entomologist

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It is certified that the thesis entitled "Cloning and characterization of genes involved in polyhydroxyalkanoates synthesis in Bacillus spp" which is submitted to the University of Mysore, Mysore, for the award of I further declare that the work presented in the thesis has not been submitted previously for the award of any degree or diploma or any other similar titles. 79: PHA production by recombinant E. coli in a fermentor with glucose as a substrate 80: Novel engineered pathway for scl-co-mcl PHA copolymer production by E. coli by E. coli strain JC7623ABC1J4

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Optimised production and utilisation of exopolysaccharide from Agrobacterium radiobacter

Cited by 110 publications

References 11 publications

Optimization of Extraction Process of Crude Protein from Grape Seeds by RSM

Optimization of Extraction Process of Crude Protein from Grape Seeds by RSM

Synthesis and Characterization of Low Molecular Weight Chitosan

Symbiosis Among Lixus scrobicollis (Coleoptera: Curculionidae), Ambrosia trifida, and Bacillus endophyticus

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