Optimization and elucidation of interactions between ammonium, nitrate and phosphate inCentella asiatica cell culture using response surface methodology

Omar, Rozita; Abdullah, Monis Abdulmanan; Hasan, M. A.; Marziah, M.; Kamal, Siti Mazlina Mustapa

doi:10.1007/bf02932012

Cited by 14 publications

(7 citation statements)

References 14 publications

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“…Asiaticoside which was detected highest at 4.26 mg g Although it is a significant factor for dry cell weight, sucrose did not affect TTPs production appreciably probably due to higher concentration range selected as the lower sucrose level (3%) had been reported to have all triterpenoids detected higher than 4 mg g −1 DW [12] . Figure 3 shows cells grown and harvested from maintenance medium (M) and growth medium (G).…”

Section: Resultsmentioning

confidence: 89%

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Development of Growth Medium for Centella Asiatica Cell Culture Via Response Surface Methodology

Omar¹,

Abdullah²,

Hasan³

et al. 2004

American J. of Applied Sciences

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Abstract:The effects of sucrose, Indole-3-Acetic Acid (IAA) and 6-BenzylAminoPurine (BAP) concentrations on cell growth of Centella asiatica cell suspension culture were studied. The concentrations were designed using Central-composite experimental design and regression analysis was carried out to obtain response surface model describing cell growth for prediction of optimum conditions. Only sucrose as a single factor was positively significant for cell growth. Increasing sucrose concentration from 3.32 to 6.68% (w/v) resulted in an increase in dry cell weight from 16-27 g L

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Section: Resultsmentioning

confidence: 89%

“…Analytical procedure and statistical analysis: The determination of cell growth and triterpenoids analysis were as described before [12] .…”

Section: Maintenance Medium and Experimental Approachmentioning

confidence: 99%

Development of Growth Medium for Centella Asiatica Cell Culture Via Response Surface Methodology

Omar¹,

Abdullah²,

Hasan³

et al. 2004

American J. of Applied Sciences

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“…Studies indicate that high NH 4 level in media has depressed calcium, potassium and magnesium uptake thereby restricting the nutrient flow into the tissues and lowers the biomass yield [24][25][26]. There is a need for a proper balance between the two nitrogen sources (NH 4 and NO 3 ) and neither of the two sources can replace each other in the media [6,27]. Prasad et al [6] reported that biomass production did not have much difference in media with total nitrogen content of 60, 40 and 20 mM.…”

Section: Total Nitrogen Concentrationmentioning

confidence: 99%

Influence of Different Macro and Micro Nutrients on the Shoot Multiplication of Centella asiatica

Ml¹,

Roy²,

Bharadvaja³

2018

J Plant Biochem Physiol

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The present study deals with the optimization of adventitious shoots cultures of Centella asiatica for maximum shoot proliferation under the influence of different nutrient manipulation. The effects of nitrogen source (NH 4 NO 3 /KNO 3), carbon (sucrose), potassium source (KNO 3), phosphorous source (KH 2 PO 4), macronutrient (MgSO 4) and micronutrient (MnSO 4) of Murashige and Skoog (MS) media on shoot growth were investigated and these experiment treatments were supplemented with 1 mg/l 6-benzylaminopurine (BAP) plant hormones. The shoot growth was influenced by relative ratio of NH 4 + :NO 3 with maximum shoot number (47.6 ± 1.52) in medium containing 100 mM (40 mM NH 4 NO 3 :60 mM KNO 3) nitrogen source when NO 3 concentration is higher than NH 4 + ions. The MS media with 3% sucrose content showed optimum shoot multiplication (36 ± 1), as with increase in carbon concentration in the media, the shoot proliferation was drastically reduced. In case of media with 30% potassium (KNO 3) highest number of shoots (31.66 ± 1.52) observed and in case of 150% phosphorous (KH 2 PO 4) highest shoot number (28 ± 1) obtained respectively. The influence of metal ions like Mg 2+ and Mn 2+ showed maximum number of shoots (31 ± 1) in media with 1.5 mM magnesium (MgSO 4) and shoot number (43 ± 2) in 200 µM manganese (MnSO 4) respectively.

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“…The response surface methodology, developed on the basis of statistically designed experiments, can provide insight into the interactions between dependent and independent variables, and can predict the optimal levels of variables. 26) The effects that can be attributed to the different factors and their interaction were assessed with maximum efficiency in a factorial design (Table 1). 27,28) The result of analysis of variance (ANOVA) showed that some of the factors examined significantly altered the stability of the bLf liquid formulation ( Table 3).…”

Section: Influence Of Additives On the Stability Of The Blf Liquid Fomentioning

confidence: 99%

Stabilization of Glycoprotein Liquid Formulation Using Arginine: A Study with Lactoferrin as a Model Protein

Kim¹,

Shin²,

Kim³

2009

Bioscience, Biotechnology, and Biochemistry

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The formulation of new biotherapeutics without human serum albumin (HSA) could decrease the potential risk of blood-transmitted diseases and those caused by infectious viruses and other pathogens. In the present study, arginine was examined as a potential alternative to HAS, and bovine lactoferrin (bLf) was used as a representative model glycoprotein since bLf has potential immunomodulatory and antiviral activity. The optimal formulation for the mixture was determined to be 10 mM arginine, 15% (w/v) trehalose, and 0.02% (v/v) Tween 80, using a statistical analysis program, Minitab. Analyses were performed using reverse-phase high-performance liquid chromatography (HPLC) and SDS-PAGE. The blf HSA-free formulations lost only 12-20% of blf compared with 46% for control (without additives) after 28 d of storage. Based on long-term stability studies, the HSA-free formulation developed in this study had a stronger effect on the stability of bLf (1.4-fold) than HSA formulation under various storage conditions over 6 months.

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Optimization and elucidation of interactions between ammonium, nitrate and phosphate inCentella asiatica cell culture using response surface methodology

Cited by 14 publications

References 14 publications

Development of Growth Medium for Centella Asiatica Cell Culture Via Response Surface Methodology

Development of Growth Medium for Centella Asiatica Cell Culture Via Response Surface Methodology

Influence of Different Macro and Micro Nutrients on the Shoot Multiplication of Centella asiatica

Stabilization of Glycoprotein Liquid Formulation Using Arginine: A Study with Lactoferrin as a Model Protein

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