Optimization and extraction of edible microbial polysaccharide from fresh coconut inflorescence sap: An alternative substrate

Mummaleti, Gopinath; Sarma, Chayanika; Kalakandan, Suresh Kumar; Vignesh, S.; Rawson, Ashish; Anandharaj, Arunkumar

doi:10.1016/j.lwt.2020.110619

Cited by 32 publications

(16 citation statements)

References 43 publications

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“…The absorption peak was located at 835.1 cm −1 and appeared in PAP1a, indicating the presence of the areca polysaccharide α-type glycosidic bond ( 35 , 36 ). The FTIR spectra of these five areca polysaccharides were similar to the corresponding coconut polysaccharides reported by Mummaleti et al, indicating that they had similar structures ( 22 ).…”

Section: Resultssupporting

confidence: 80%

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Structural Elucidation and Antioxidant Activities of a Neutral Polysaccharide From Arecanut (Areca catechu L.)

Guo

Pan³

et al. 2022

Front. Nutr.

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A novel neutral polysaccharide designated as PAP1b was isolated from Areca catechu L. by hot water extraction, ethanol precipitation, and column chromatography. PAP1b was mainly composed of mannose, galactose, xylose, and arabinose in a ratio of 4.1:3.3:0.9:1.7, with an average molecular weight of 37.3 kDa. Structural characterization indicated that the backbone of PAP1b appeared to be composed mainly of → 6-β-Manp-(1 →, → 4)-α-Galp-(1 → and → 3,6)-β-Manp-(1 →) residues with some branches, and terminal of (1 →)-linked-β-Manp residues. The results of bioactivity experiments showed that PAP1b had antioxidant in vitro, esspecially on scavenging DPPH and hydroxyl radicals. Therefore, the polysaccharide from Areca catechu L. could be used as a potential antioxidant in functional food.

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Section: Resultssupporting

confidence: 80%

“…The molecular weight of various arecanut (Areca chu L.) polysaccharides may be different in the range of 10 4 -10 6 Da. The Mws of neutral and acidic polysaccharides were similar to coconut inflorescence polysaccharides obtained by Mummaleti et al (22).…”

Section: Extraction Purification and Fractionation Of Polysaccharidessupporting

confidence: 84%

Structural Elucidation and Antioxidant Activities of a Neutral Polysaccharide From Arecanut (Areca catechu L.)

Guo

Pan³

et al. 2022

Front. Nutr.

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“…As displayed, the F value of 39.34 and p value of <0.0001 indicated a highly significant model. The p value of lack of fit was 0.7268, which also suggested a fitted model [ 28 ]. Besides, statistical indicators, including R 2 , Adj R 2 , Pred R 2 , and CV values, were 0.9752, 0.9504, 0.8935, and 3.18, respectively, and the difference of Pred R 2 and Adj R 2 was less than 0.2 (0.0569), indicating a good correlation between actual and predicted values [ 29 ].…”

Section: Resultsmentioning

confidence: 98%

Preparation, Antioxidant and Immunoregulatory Activities of a Macromolecular Glycoprotein from Salvia miltiorrhiza

Dai

Liu

et al. 2022

Foods

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Salvia miltiorrhiza has exhibited various bioactive functions due to the existence of polysaccharides, hydrophilic phenolic acids, diterpenoid quinones, and essential oils. However, little research has reported the glycoprotein preparation and corresponding bioactivities. In this study, the water-soluble glycoprotein from S. miltiorrhiza roots was firstly isolated with the extraction process optimized by response surface methodology, and then, the preliminary structural properties, and the antioxidant and immunoregulatory activities were investigated. Results showed that the extraction conditions for higher extraction yields were identified as follows: ultrasonic power of 220 W, ultrasonic time of 2.0 h, extraction temperature of 60 °C, liquid/solid ratio of 20 mL/g, and the glycoprotein yields of 1.63 ± 0.04%. Structural analysis showed that the glycoprotein comprised protein and polysaccharide (contents of 76.96% and 20.62%, respectively), with an average molecular weight of 1.55 × 105 Da. Besides, bioactivities analysis showed that the glycoprotein presented strong scavenging effects on multiple free radicals, and effectively enhanced the antioxidant enzyme activities and immunological indicators in cyclophosphamide-induced immunocompromised mice dose-dependently. These data demonstrated that S. miltiorrhiza glycoprotein presented the potential to be a novel edible functional compound, and could be practically applied in the food industry.

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“…The variance analysis demonstrated that coefficients A , B , C , AC , BC , A 2 , B 2 , and C 2 were extremely significant ( p < 0.01), whereas the interaction effects of AB showed no significant difference on cCPP yields, which indicates that the variables A , B , C , AC , BC , A 2 , B 2 , and C 2 could significantly influence the extraction yields of cCPPs. As shown, the p < 0.0001 of this mode and the p = 0.7258 for the lack-of-fit value indicate insignificant effects of deviation and suggest a fitted model [ 32 ]. Besides, the difference of less than 0.2 between the Pred R 2 and Adj R 2 values also indicates a good correlation between the predicted and actual cCPP extraction yields [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

Ultrasonic-Assisted Extraction of Codonopsis pilosula Glucofructan: Optimization, Structure, and Immunoregulatory Activity

Jiao

et al. 2022

Nutrients

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In recent years, multiple edible polysaccharides from Codonopsis pilosula were mainly isolated with high average molecular weights and exhibited various bioactivities, but it was proven that low-molecular-weight polysaccharides could exert stronger activities due to the superior water solubility and permeability. In the present study, the water-soluble polysaccharide C. pilosula with low molecular weight was isolated under ultrasonic assistance at 30 °C, the extraction process was optimized via response surface method (RSM), and the structure and immunoregulatory activity were further investigated. The maximum yield (4.86%) for crude polysaccharides (cCPPs) was obtained under following parameters: ultrasonic power of 370 W, liquid/material ratio of 33 mL/g, ultrasonic time of 81 min. Subsequently, the cCPPs were further purified through dialysis and Sephadex G-25 column to acquire purified polysaccharide (CPPs). Structural analysis indicated that CPPs was a glucofructan (average molecular weight of 4.23 × 103 Da) with (2→1)-β-D-Fruf and (1→)-α-D-Glcp as the backbone branched by (2→6)-β-D-Fruf. Additionally, CPPs could enhance immunoregulatory function by stimulating NO production and cytokine (IL-6 and TNF-α) secretion of RAW264.7 macrophages dose-dependently, which presented no cytotoxic effects. These data suggest that CPPs have the potential to be used as a nutritional dietary compound and natural immunostimulant supplement in the food industry.

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Optimization and extraction of edible microbial polysaccharide from fresh coconut inflorescence sap: An alternative substrate

Cited by 32 publications

References 43 publications

Structural Elucidation and Antioxidant Activities of a Neutral Polysaccharide From Arecanut (Areca catechu L.)

Structural Elucidation and Antioxidant Activities of a Neutral Polysaccharide From Arecanut (Areca catechu L.)

Preparation, Antioxidant and Immunoregulatory Activities of a Macromolecular Glycoprotein from Salvia miltiorrhiza

Ultrasonic-Assisted Extraction of Codonopsis pilosula Glucofructan: Optimization, Structure, and Immunoregulatory Activity

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