“…The slope of the 158 pipeline in the simulator was set to 0.8% to facilitate gravity draining. 159 The alkaline and acidic EO water solutions were generated using an EO water generator soiling procedure as suggested by our previous study (Dev et al, 2014), i.e., one third (12.7 L) 176 of the raw milk followed by a 10 min air dry, repeated three times. After the soiling was 177 completed, the specimens were dried in an incubator (Model Symphony, VWR international, 178 Radnor, PA), cooled in the desiccator (Model Dry-keeper AutoA-3B, Sanplatec Corp., Osaka, 179 Japan) then weighed using a high precision balance (Model XP504, Mettler-Toledo, LLC., 180 Columbus, OH) with a weighing range of 0 -520 g and a readability of +/-0.0001 g. The 181 deposit mass was determined by subtracting the measured mass of the stainless steel 182 specimen before and after each experiment.…”