Optimization and scale up of production of alkaline protease from Conidiobolus coronatus

Laxman, R. Seeta; Sonawane, Atul P.; More, Snehal; Rao, B.S. Shankaranarayana; Rele, M. V.; Jogdand, V. V.; Deshpande, Vasanti; Rao, Mala

doi:10.1016/j.procbio.2005.04.005

Cited by 101 publications

(45 citation statements)

References 13 publications

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“…It is also known that 30-40% of the production cost of industrial enzymes is estimated to be the cost of growth medium [24]. These sources together with factors like agitation speed and inoculation ratio, besides their effect on the product formation, have been determined to play significant role in the determination of the final morphology of the culture [25].…”

Section: Resultsmentioning

confidence: 99%

Optimization of biomass, pellet size and polygalacturonase production by Aspergillus sojae ATCC 20235 using response surface methodology

Tarı

Gogus

Tokatlı

2007

Enzyme and Microbial Technology

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A two-step optimization procedure using central composite design with four factors (concentrations of maltrin and corn steep liquor (CSL), agitation speed and inoculation ratio) was used in order to investigate the effect of these parameters on the polygalacturonase (PG) enzyme activity, mycelia growth (biomass) and morphology (pellet size) of Aspergillus sojae ATCC 20235. According to the results of response surface methodology (RSM), initial concentrations of maltrin and CSL and agitation speed were significant (p < 0.05) on both PG enzyme production and biomass formation. As a result of this optimization, maximum PG activity (13.5 U/ml) was achievable at high maltrin (120 g/l), at low CSL (0 g/l), high agitation speed (350 rpm) and high inoculation ratio (2 × 10 7 total spore). Similarly, maximum biomass (26 g/l) could be obtained under the same conditions with only the difference for higher level of CSL requirement. The diameter of pellets in all optimization experiments ranged between 0.05 and 0.76 cm. The second optimization step improved the PG activity by 74% and the biomass by 40%.

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Section: Resultsmentioning

confidence: 99%

Optimization of biomass, pellet size and polygalacturonase production by Aspergillus sojae ATCC 20235 using response surface methodology

Tarı

Gogus

Tokatlı

2007

Enzyme and Microbial Technology

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“…It is important to produce the enzyme in inexpensive and optimized media on large-scale for the process to be made commercially viable and also to study the influence of various parameters on enzyme production (SeetaLaxman et al 2005). The production and timing of protease activity in the fermentations were investigated with respect to optimum agitation rate.…”

Section: Effect Of Ph and Agitation Rates In Scale Up Fermentationmentioning

confidence: 99%

Scale up of production in a bioreactor of a halotolerant protease from moderately halophilic Bacillus sp. isolated from soil

Prasad

Abraham²,

Nair

2014

Braz. arch. biol. technol.

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“…The significant effect of soy flour as a nitrogen source for protease production by B. subtilis C4 corroborates a report of Joo and Chang 23 showing the preference of Bacillus spp. for soy flour, which has also been reported as an inducer for alkaline protease from Conidiobolus coronatus 24 as well. Casein, a component of skimmed milk, has also been reported as an inducer of this enzyme 25 .…”

Section: Runmentioning

confidence: 83%

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Romsomsa¹,

Chim-anagae²,

Jangchud³

2010

ScienceAsia

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Bacillus subtilis C4 was isolated from waste water of a Thai-silk dyeing factory, and identified as a potent strain for producing silk-degumming protease. To optimize the protease production of this bacterial strain, seven fermentation variables were screened using a Plackett-Burman design, and were then further optimized via response surface methodology based on a central composite design. Three significant variables, i.e., soy flour, skimmed milk, and shaker speed, were selected. The optimal values were 2.0% soy flour, 0.1% skimmed milk, and a shaker speed of 280 rpm. The experimental result (1537 units/ml) in a medium optimized for protease production was in good agreement with the predicted value of a quadratic model (1576 units/ml), thus confirming its validity. In addition, the adequacy of the model was supported by a coefficient of determination (R 2 ) of 0.912. Protease production in the optimized medium (1537 units/ml) increased 2.2-fold over that of the non-optimized medium (729 units/ml) in the shaken flask culture. When the experiment was scaled up in a stirred tank reactor, 1891 units/ml protease activity was achieved at 27 h of cultivation, which was an overall 2.6-fold increase over the basal medium.

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Optimization and scale up of production of alkaline protease from Conidiobolus coronatus

Cited by 101 publications

References 13 publications

Optimization of biomass, pellet size and polygalacturonase production by Aspergillus sojae ATCC 20235 using response surface methodology

Optimization of biomass, pellet size and polygalacturonase production by Aspergillus sojae ATCC 20235 using response surface methodology

Scale up of production in a bioreactor of a halotolerant protease from moderately halophilic Bacillus sp. isolated from soil

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