Optimization and scale-up of l-lactic acid fermentation by mutant strain Rhizopus sp. MK-96-1196 in airlift bioreactors

Miura, Shigenobu; Arimura, Tomohiro; Hoshino, Minako; KOJIMA, MAMI; Dwiarti, Lies; Okabe, Mitsuyasu

doi:10.1016/s1389-1723(03)90098-3

Cited by 59 publications

(39 citation statements)

References 9 publications

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“…Inoculum was made by spores (10 6 .mL -1 ), obtained in YPG agar from Roux bottles [20]. Cultures were made in 1 L flasks adapted with an aeration system, as described: aeration was made with a 0.33 vvm air flow, measured in a flowmeter (Vögtlin Instruments GmbH,Aesch, Switzerland), in a range of 1.6 -16.0 Nl.h -1 .…”

Section: Culture Mediamentioning

confidence: 99%

Chitosans from Rhizopus stolonifer (strain CBMAI 1551): Characterization and Dense Film Formation

Cardoso

ConceiÃ§Ã£o

Giachini

et al. 2017

JBT

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I S S N 2348-6201 V o l u m e 6 N u m b e r 3 J o u r n a l o f A d v a n c e i n B i o t e c h n o l o g y | ABSTRACTChitosan is a bioactive amino polymer with wide applications. Mainly derived from chitin of marine sources, its traditional production still has some drawbacks such as irregular supply, low quality of product and lack of standardization. Farther, extraction processes are time-consuming with considerable environmental impacts, an extremely non-green process. Many works have shown the possibility of producingnative chitosan from Mucorales fungi, which is more easily extracted. Such process is advantageous due to low costs, process control and great possibility of high quality products. Moreover, the extraction of chitosan is faster and generates less pollutants. In this scenario, the possibility of standardized production allied with facilitated extraction and less probability of toxicological side effects from marine sources are characteristics that motivated this work. A Mucoralesisolate was cultured and the chitosans -native and semi-synthetic -obtained through heterogeneous extraction were compared. Results show substantial differences between them. Those differences are related to the processes required for extraction, yield, productivity, and quality. This work reinforces that Mucorales fungi excel as an alternative for chitosan production.

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Section: Culture Mediamentioning

confidence: 99%

Chitosans from Rhizopus stolonifer (strain CBMAI 1551): Characterization and Dense Film Formation

Cardoso

ConceiÃ§Ã£o

Giachini

et al. 2017

JBT

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“…It should be noted that there were no pellets but rather small, short mycelia in the seed culture. However, in the research of Miura et al [13], uniform pellets preformed in the seed culture. That study also noted that inoculum size had a signiWcant eVect on L-lactic acid yield, and especially on R. oryzae AS 3.819 morphology.…”

Section: Inoculum Sizementioning

confidence: 99%

Production of l-lactic acid by Rhizopus oryzae using semicontinuous fermentation in bioreactor

Jiang

Liu

et al. 2010

J Ind Microbiol Biotechnol

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Semicontinuous fermentation using pellets of Rhizopus oryzae has been recognized as a promising technology for L-lactic acid production. In this work, semicontinuous fermentation of R. oryzae AS 3.819 for L-lactic acid production has been developed with high L-lactic acid yield and volumetric productivity. The effects of factors such as inoculations, CaCO₃ addition time, and temperature on L-lactic acid yield and R. oryzae morphology were researched in detail. The results showed that optimal fermentation conditions for the first cycle were: inoculation with 4% spore suspension, CaCO₃ added to the culture medium at the beginning of culture, and culture temperature of 32-34 °C. In orthogonal experiments, high L-lactic acid yield was achieved when the feeding medium was (g/l): glucose, 100; (NH₄)₂SO₄, 2; KH₂PO₄, 0.1; ZnSO₄·7H₂O, 0.33; MgSO₄·7H₂O, 0.15; CaCO₃, 50. Twenty cycles of semicontinuous fermentation were carried out in flask culture. L: -lactic acid yield was 78.75% for the first cycle and 80-90% for the repeated cycles; the activities of lactate dehydrogenases (LDH) were 7.2-9.2 U/mg; fermentation was completed in 24 h for each repeated cycle. In a 7-l magnetically stirred fermentor, semicontinuous fermentation lasted for 25 cycles using pellets of R. oryzae AS 3.819 under the optimal conditions determined from flask cultures. The final L-lactic acid concentration (LLAC) reached 103.7 g/l, and the volumetric productivity was 2.16 g/(l·h) for the first cycle; in the following 19 repeated cycles, the final LLAC reached 81-95 g/l, and the volumetric productivities were 3.40-3.85 g/(l·h).

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“…Correlations that can adequately predict the kLa values in a particular process are fundamental for bioreactor scaling up [2,5,18,19]. A correlation for the kLa can be obtained as a function of the superficial gas velocity {kLa=α[1+Ad/Ar] -2 (Ug) ß } [20], such as the one presented in Fig.…”

Section: Volumetric Oxygen Transfer Coefficientmentioning

confidence: 99%

Airlift Bioreactor Fluid-Dynamic Characterizationfor the Cultivation of Shear Stress Sensitivemicroorganisms

Rossi

Nascimento

Giachini

et al. 2015

JBT

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ISSN 2 3 4 8 -6 2 0 1 V o l u m e 5 N u m b e r 2 J o u r n a l o f A d v a n c e s i n B i o t e c h n o l o g y 639 | P a g e c o u n c i l f o r I n n o v a t i v e R e s e a r c h F e b r u a r y 2 0 1 6 w w w . ABSTRACTAirlift bioreactors are considered very efficient for aerobic cultivation of microorganisms. In particular, for the cultivation of filamentous fungi, where low shear rates may be required. In this context, the main aim of this study was to design an airlift bioreactor built with an external loop, adequate for biomass production of microbial organisms, including ectomycorrhizal fungi. A 5-L airlift bioreactor was constructed and experimental studies performed under an air-salt solution system (0.15 mol/L NaCl), in order to characterize the reactor´s fluid-dynamic in relation to the superficial gas velocity (Ug) in the range of 0.001 to 0.020 m/s. In order to evaluate the performance of the reactor, a preliminary assay was conducted with the ectomycorrhizal fungus Rhizopogon nigrescens. Better gas separation reflected in better liquid circulation and higher oxygen transfer (0.0197 1/s at 1 vvm) when compared to an operating, 2.3-L prototype airlift. According to this study, volumetric coefficients for oxygen transfer (kLa) up to 0.020 1/s (specific airflow rates of 1.0 vvm) are sufficient to promote the growth of shear stress sensitive microorganisms, such as ectomycorrhizal fungi.

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Optimization and scale-up of l-lactic acid fermentation by mutant strain Rhizopus sp. MK-96-1196 in airlift bioreactors

Cited by 59 publications

References 9 publications

Chitosans from Rhizopus stolonifer (strain CBMAI 1551): Characterization and Dense Film Formation

Chitosans from Rhizopus stolonifer (strain CBMAI 1551): Characterization and Dense Film Formation

Production of l-lactic acid by Rhizopus oryzae using semicontinuous fermentation in bioreactor

Airlift Bioreactor Fluid-Dynamic Characterizationfor the Cultivation of Shear Stress Sensitivemicroorganisms

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