Optimization and Validation for Determination of Volatile Organic Compounds from Mediterranean Fruit Fly (Medfly) Ceratitis capitata (Diptera: Tephritidae) by Using HS-SPME-GC-FID/MS

Al-Khshemawee, Hasan; Agarwal, Manjree; Ren, Y.

doi:10.3923/jbs.2017.347.352

Cited by 6 publications

(5 citation statements)

References 17 publications

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“…Other intermediate to low-concentration components may also be required to attain full parity with calling males. Flath et al [ 29 ]; Al-khshemawee et al [ 30 ] reported that three different medfly ages (5–6, 11–12, and 20–21 days old), and early-, mid-, and late-morning samples were used to collect volatiles. Thirty-two components were identified.…”

Section: Resultsmentioning

confidence: 99%

Application of Direct Immersion Solid-Phase Microextraction (DI-SPME) for Understanding Biological Changes of Mediterranean Fruit Fly (Ceratitis capitata) During Mating Procedures

Al-Khshemawee

Agarwal

et al. 2018

Molecules

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Samples from three different mating stages (before, during and after mating) of the Mediterranean fruit fly Ceratitis capitata were used in this experiment. Samples obtained from whole insects were subjected to extraction with the two mixtures of solvents (acetonitrile/water (A) and methanol/acetonitrile/water (B)) and a comparative study of the extractions using the different solvents was performed. Direct immersion-solid phase microextraction (DI-SPME) was employed, followed by gas chromatographic-mass spectrometry analyses (GC/MS) for the collection, separation and identification of compounds. The method was validated by testing its sensitivity, linearity and reproducibility. The main compounds identified in the three different mating stages were ethyl glycolate, α-farnesene, decanoic acid octyl ester, 2,6,10,15-tetramethylheptadecane, 11-tricosene, 9,12-(Z,Z)-octadecadienoic acid, methyl stearate, 9-(Z)-tricosene, 9,11-didehydro-lumisterol acetate; 1,54-dibromotetrapentacontane, 9-(Z)-hexadecenoic acid hexadecyl ester, 9-(E)-octadecenoic acid and 9-(Z)-hexadecenoic acid octadecyl ester. The novel findings indicated that compound compositions were not significantly different before and during mating. However, new chemical compounds were generated after mating, such as 1-iodododecane, 9-(Z)-tricosene and 11,13-dimethyl-12-tetradecen-1-acetate which were extracted with both (A) and (B) and dodecanoic acid, (Z)-oleic acid, octadecanoic acid and hentriacontane which were extracted with (A) and ethyl glycolate, 9-hexadecenoic acid hexadecyl ester, palmitoleic acid and 9-(E)-octadecenoic acid, which were extracted with solvent (B). This study has demonstrated that DI-SPME is useful in quantitative insect metabolomics by determining changes in the metabolic compounds in response to mating periods. DI-SPME chemical extraction technology might offer analysis of metabolites that could potentially enhance our understanding on the evolution of the medfly.

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Section: Resultsmentioning

confidence: 99%

Application of Direct Immersion Solid-Phase Microextraction (DI-SPME) for Understanding Biological Changes of Mediterranean Fruit Fly (Ceratitis capitata) During Mating Procedures

Al-Khshemawee

Agarwal

et al. 2018

Molecules

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“…Five and 30 day-old males have been used, with 36 and 27 chemicals (mostly belonging to terpenes, amides, esters and alkanes) identified to be emitted by these two age-classes, respectively [219]. In a recent work on C. capitata volatiles, divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) combined with GC-MS and gas chromatography-flame ionization detection (GC-FID) techniques resulted in the identification of 27, 23 and 29 compounds from larvae, pupae and adults, respectively [264]. PDMS/DVB SPME fibres have been applied to collect volatiles produced by B. zonata males and females and R. batava males, respectively [60,212].…”

Section: Volatile Collection and Analytical Techniques For Their Iden...mentioning

confidence: 99%

From Insect Pheromones to Mating Disruption

2022

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“…Five and 30 day-old males have been used, with 36 and 27 chemicals (mostly belonging to terpenes, amides, esters and alkanes) identified to be emitted by these two age-classes, respectively [ 220 ]. In a recent work on C. capitata volatiles, divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) combined with GC-MS and gas chromatography-flame ionization detection (GC-FID) techniques resulted in the identification of 27, 23 and 29 compounds from larvae, pupae and adults, respectively [ 266 ]. PDMS/DVB SPME fibres have been applied to collect volatiles produced by B. zonata males and females and R. batava males, respectively [ 61 , 213 ].…”

Section: Volatile Pheromonesmentioning

confidence: 99%

Tephritid Fruit Fly Semiochemicals: Current Knowledge and Future Perspectives

Scolari

Valerio

Benelli

et al. 2021

Insects

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The Dipteran family Tephritidae (true fruit flies) comprises more than 5000 species classified in 500 genera distributed worldwide. Tephritidae include devastating agricultural pests and highly invasive species whose spread is currently facilitated by globalization, international trade and human mobility. The ability to identify and exploit a wide range of host plants for oviposition, as well as effective and diversified reproductive strategies, are among the key features supporting tephritid biological success. Intraspecific communication involves the exchange of a complex set of sensory cues that are species- and sex-specific. Chemical signals, which are standing out in tephritid communication, comprise long-distance pheromones emitted by one or both sexes, cuticular hydrocarbons with limited volatility deposited on the surrounding substrate or on the insect body regulating medium- to short-distance communication, and host-marking compounds deposited on the fruit after oviposition. In this review, the current knowledge on tephritid chemical communication was analysed with a special emphasis on fruit fly pest species belonging to the Anastrepha, Bactrocera, Ceratitis, and Rhagoletis genera. The multidisciplinary approaches adopted for characterising tephritid semiochemicals, and the real-world applications and challenges for Integrated Pest Management (IPM) and biological control strategies are critically discussed. Future perspectives for targeted research on fruit fly chemical communication are highlighted.

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Optimization and Validation for Determination of Volatile Organic Compounds from Mediterranean Fruit Fly (Medfly) Ceratitis capitata (Diptera: Tephritidae) by Using HS-SPME-GC-FID/MS

Cited by 6 publications

References 17 publications

Application of Direct Immersion Solid-Phase Microextraction (DI-SPME) for Understanding Biological Changes of Mediterranean Fruit Fly (Ceratitis capitata) During Mating Procedures

Application of Direct Immersion Solid-Phase Microextraction (DI-SPME) for Understanding Biological Changes of Mediterranean Fruit Fly (Ceratitis capitata) During Mating Procedures

From Insect Pheromones to Mating Disruption

Tephritid Fruit Fly Semiochemicals: Current Knowledge and Future Perspectives

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