Optimization and validation method to evaluate the residues of β-lactams and tetracyclines in kidney tissue by UPLC–MS/MS

Almeida, M P; Rezende, Cristiana Perdigão; Ferreira, Flavia Domingues; Souza, Leonardo Francisco de; Assis, Débora Cristina Sampaio de; Figueiredo, Tadeu Chaves de; Leite, Mônica de Oliveira; Cançado, Silvana de Vasconcelos

doi:10.1016/j.talanta.2015.07.048

Cited by 19 publications

(11 citation statements)

References 20 publications

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“…With the indiscriminate use of ENR, the presence of its residues in animals may lead to adverse health effects on human, such as allergic reactions and ENR-resistant strains. In addition, sub-therapeutic metabolites of quinolones can persist in edible tissues and be ingested by human, and resistance genes may be transferred to endogenous or exogenous bacterium (Sa et al, 2008; Aliu and Sulaj, 2014; de Almeida et al, 2015; Wang et al, 2016). As a quinolone commonly used in poultry and mammalian production, ENR has a direct effect of inhibiting bacterial DNA-gyrase and topoisomerase IV enzyme activities (Ebrahimzadeh et al, 2014; Gouvêa et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Clinical Efficacy and Residue Depletion of 10% Enrofloxacin Enteric-Coated Granules in Pigs

et al. 2017

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A new, more palatable formulation of 10% enrofloxacin enteric-coated granules was investigated to evaluate the pharmacokinetic effect in plasma, the residue elimination in tissues and the clinical efficacy against Actinobacillus pleuropneumonia (APP) and Mycoplasam suis (MS) in pigs. In this study, the enrofloxacin concentrations in plasma and tissues were detected using high-performance liquid chromatography with phosphate buffer (pH = 3) and acetonitrile. The pharmacokinetics and elimination of enrofloxacin enteric-coated granules were performed after oral administration at a single dose of 10 mg/kg body weight (bw) and 5 mg/kg twice per day for 5 consecutive days, respectively. The in vivo antibacterial efficacy and clinical effectiveness of enrofloxacin enteric-coated granules against APP and MS were assayed at 2.5, 5, 10 mg/kg, compared with tiamulin (8 mg/kg) based on establishment of APP and MS infection models. 56 APP strains were selected and tested for in vitro antibacterial activity of enrofloxacin enteric-coated granules. The main parameters of elimination half-life (t1/2β), Tmax, and area under the curve (AUC) were 14.99 ± 4.19, 3.99 ± 0.10, and 38.93 ± 1.52 μg h/ml, respectively, revealing that the enrofloxacin concentration remained high and with a sustainable distribution in plasma. Moreover, the analysis on the evaluation of enrofloxacin and ciprofloxacin in muscle, fat, liver and kidney showed that the recovery were more than 84% recovery in accordance with the veterinary drug residue guidelines of United States pharmacopeia, and the withdrawal periods were 4.28, 3.81, 4.84, and 3.51 days, respectively, suggesting that the withdrawal period was 5 d after oral administration of 5 mg/kg twice per day. The optimal dosage of enrofloxacin enteric-coated granules against APP and MS was 5 mg/kg, with over 90% efficacy, which was significantly different (p < 0.05) to the 2.5 mg/kg group, but not to the 10 mg/kg group or the positive control group (tiamulin). In conclusion, 10% enrofloxacin enteric-coated granules had significant potential for treating APP and MS, and it provided an alternative enrofloxacin palatability formulation.

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Section: Introductionmentioning

confidence: 99%

Clinical Efficacy and Residue Depletion of 10% Enrofloxacin Enteric-Coated Granules in Pigs

et al. 2017

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“…These antimicrobials are widely used in poultry production as therapeutic or prophylactic agents because broiler chickens are raised in intensive industrial farming systems, the stressful conditions of which can make these animals more susceptible to infectious diseases [1,2]. However, the indiscriminate use of antimicrobials may lead to the presence of their residues in food products of animal origin.…”

Section: Introductionmentioning

confidence: 99%

“…However, the indiscriminate use of antimicrobials may lead to the presence of their residues in food products of animal origin. These residues may have adverse health effects in humans, such as allergic reactions, and may lead to selection for bacterial strains that are resistant to antibiotics, especially when there is a lack of compliance with the withdrawal period determined for each medication [2–8]. …”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Presence and Levels of Enrofloxacin, Ciprofloxacin, Sulfaquinoxaline and Oxytetracycline in Broiler Chickens after Drug Administration

et al. 2016

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The depletion times of enrofloxacin and its metabolite ciprofloxacin as well as sulfaquinoxaline and oxytetracycline were evaluated in broiler chickens that had been subjected to pharmacological treatment. The presence and residue levels of these drugs in muscle tissue were evaluated using an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method that was validated in this work. The results showed the presence of all antimicrobial residues; however, the presence of residues at concentrations higher than the drugs’ maximum residue limit (MRL) of 100 μg kg-1 was found only during the treatment period for oxytetracycline and until two days after discontinuation of the medication for enrofloxacin, ciprofloxacin and sulfaquinoxaline. It was concluded that the residues of all antimicrobials were rapidly metabolized from the broiler muscles; after four days of withdrawal, the levels were lower than the limit of quantification (LOQ) of the method for the studied analytes.

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“…, the most commonly used tetracyclines are oxytetracycline (OTC), tetracycline (TC), chlortetracycline (CTC) and doxycycline (DC), whose structures are quite similar. The problem caused by abuse of antimicrobial reagents has stimulated the development of analytical technology for the determination of TCs residues, such as LC/MS , CE/MS , HPLC , CZE and chemiluminescence .…”

Section: Introductionmentioning

confidence: 99%

β‐cyclodextrin‐ionic liquid polymer based dynamically coating for simultaneous determination of tetracyclines by capillary electrophoresis

et al. 2017

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Tetracyclines are a group of broad spectrum antibiotics widely used in animal husbandry to prevent and treat diseases. However, the improper use of tetracyclines may result in the presence of their residues in animal tissues or waste. Recently, great attention has been drawn towards the green solvents ionic liquids. Ionic liquids have been employed as a coating material to modify the electroosmotic flow in capillary electrophoresis. In this study, a functionalized ionic liquid, mono-6-deoxy-6-(3-methylimidazolium)-β-cyclodextrin tosylate, was synthesized and used for the simultaneous separation and quantification of tetracyclines by capillary electrophoresis. Good separation efficiency could be achieved due to the multiple functions of β-cyclodextrin derived ionic liquid, including the electrostatic interaction, the hydrogen bonding, and the cavity structure in β-cyclodextrin ionic liquid which can entrap the tetracyclines to form inclusion complex. After optimization, baseline separation achieved in 25 min with the running buffer consisted of 10 mmol/L, pH 7.2 phosphate buffer and 20 mmol/L β-cyclodextrin ionic liquid. The satisfied result demonstrated that the β-cyclodextrin ionic liquid is an ideal background electrolyte modifier in the separation of tetracyclines with high stability and good reproducibility. And it is an effective strategy to design and synthesize specific ILs as additive applied in separation.

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Optimization and validation method to evaluate the residues of β-lactams and tetracyclines in kidney tissue by UPLC–MS/MS

Cited by 19 publications

References 20 publications

Clinical Efficacy and Residue Depletion of 10% Enrofloxacin Enteric-Coated Granules in Pigs

Clinical Efficacy and Residue Depletion of 10% Enrofloxacin Enteric-Coated Granules in Pigs

Evaluation of the Presence and Levels of Enrofloxacin, Ciprofloxacin, Sulfaquinoxaline and Oxytetracycline in Broiler Chickens after Drug Administration

β‐cyclodextrin‐ionic liquid polymer based dynamically coating for simultaneous determination of tetracyclines by capillary electrophoresis

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