Optimization Extraction Conditions for Phenolic Compounds, Antioxidant and Inhibitory Activities of Angiotensin I-Converting Enzyme (ACE), α-Glucosidase and α-Amylase fromMentha Spicata L

Abstract

Ideal extraction conditions were estimated based on the maximum phenolic content, antioxidant activity and inhibitory activity of angiotensin-converting enzyme (ACE), α-glucosidase and α-amylase enzymes from Mentha spicata L. The optimum extraction conditions for phenolic compounds with maximum ACE inhibitory activity occurred in a mixture of acetone and water (1:1) at 40C for 6 h. The optimal extraction conditions for phenolic compounds with maximum α-amylase and α-glucosidase inhibitory activity were obtained in a mixture of methanol, acetone and water extraction (1:1:1) and in a mixture of methanol and distilled water (1:1) at 50C for 8 h. The major free phenolic compound extracted with the various extraction solvents, times and temperatures was rutin. The predominant bound phenolic compounds were caffeic acid and chlorogenic acid, which did not exist as free phenolic compounds. PRACTICAL APPLICATIONSThis study suggested the optimum extraction conditions of phenolic compounds with maximum capacity of antioxidant, antihypertensive and antidiabetic properties recommended to be used in food industry as pharmaceutical and nutraceutical herbal extract.

Chemical Profile and Bioactivities of Extracts from Edible Plants Readily Available in Portugal

“…Mentha species, which include spearmint ( Mentha spicata L.), have a long history of use in the treatment of respiratory problems (such as bronchitis) and digestive issues (nausea, ulcerative colitis, flatulence, etc.) [ 18 ]. The medicinal effects of Mentha species include anticatarrhal, anti-inflammatory, carminative, antiemetic, diaphoretic, antimutagenicity, antispasmodic, antioxidant, and analgesic activities [ 18 ].…”

“…[ 18 ]. The medicinal effects of Mentha species include anticatarrhal, anti-inflammatory, carminative, antiemetic, diaphoretic, antimutagenicity, antispasmodic, antioxidant, and analgesic activities [ 18 ].…”

Antidiabetic bio-peptides of soft and hard wheat glutens

“…These results confirm that the size of the molecular peptide could affect the α-amylase inhibitory activity. Aludatt et al (2016) found that the highest level of α-amylase inhibitory activity (66%) was obtained from prolamine peptides of barley protein fractions and the lowest values exhibited in glutenin fraction. Ngoh and Gan (2016) also confirmed that pinto bean peptides (<3 kDa) had a very good α-amylase inhibitory activity (62.1 ± 3.5%) but larger polypeptides ( M w > 100 kDa) exhibited the lowest inhibition.…”

Review on Health Benefits of Spearmint (Mentha spicata L.) and Its Inter-cropping Advantage with Maize (Zea mays L.)

“…The contents of phenolic compounds in spearmint oil are 8.9mg/100g for caffeic acid, 1344mg/100g for rosmarinic acid, and 16.8mg/100 g for luteolin [62]. Many volatile compounds have been identified in spearmint including flavonoid, thymonin, carvone, and limonene [3]. Reported that extracted phenolic compounds from spearmint have been used for medical purposes.…”

“…For instance, nausea, vomiting, and gastrointestinal disorders [26,36]. Also, spearmint flavonoids, thymonin, caffeic acid, rosmaric acid, carvone, and limonene, have been used for medical purposes to address such issues as smooth muscle spasms, increase blood circulation, relieve pain, antiseptic, morning sickness, nausea, menstrual cramps, flatulence, flu, and vomiting [3]. Recently, phenolic compounds in mint have been investigated to repair DNA damage in cancer.…”