Optimization for the production of exopolysaccharides from Morchella esculenta SO-02 in submerged culture and its antioxidant activities in vitro

Meng, Fanyun; Liu, Xiaonan; Liu, Jia; Song, Zilin; Deng, Peng; Fan, Keming

doi:10.1016/j.carbpol.2009.09.032

Cited by 49 publications

(16 citation statements)

References 22 publications

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“…Link and other ascomycetes. The reason for this is low oxygen requirement by the culture at 150 rpm rotation [27, 37]. The results obtained under present experiments on the effect of rotation speed on polysaccharide production were the same as those obtained in C. ophioglossoides [37].…”

Section: Resultssupporting

confidence: 71%

“…Polysaccharides extracted from Cordyceps species were known to exhibit multiple pharmacological activities including antitumor, anti-inflammatory, immunopotentiation, hypoglycemic, and hypocholesterolemic effects, protection of neuronal cells against the free radical-induced cellular toxicity, steroidogenesis, and antioxidant activities [21–27]. Polysaccharides isolated from the mycelia of Cordyceps fungi have been known to possess antioxidant properties which provide health benefits in preventing damage due to free radicals produced by biological degeneration [28–32].…”

Section: Introductionmentioning

confidence: 99%

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Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture ofCordyceps gracilis(Grev.) Durieu & Mont.

Sharma

Gautam

Atri

2015

Evidence-Based Complementary and Alternative Medicine

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Under present experiments, EPS and IPS production, monosaccharide composition, and antioxidant activities of C. gracilis were studied for the first time under submerged culture conditions. Effect of different factors on polysaccharides production was studied by orthogonal experiments using one-factor-at-a-time method. Incubation of culture in the medium with capacity 200 mL (675.12 ± 5.01 and 385.20 ± 5.01 mg/L), rotation speed 150 rpm (324.62 ± 3.32 and 254.62 ± 4.62 mg/L), 6-day culture incubation time (445.24 ± 1.11, 216.60 ± 1.71 mg/L), pH 6.0 (374.81 ± 2.52 and 219.45 ± 2.59 mg/L), and temperature 23°C (405.24 ± 1.11 and 215.60 ± 1.71 mg/L) produced higher EPS and IPS, respectively. Maximum EPS and IPS production was observed in the medium supplemented with glucose as a carbon source (464.82 ± 2.12 and 264.42 ± 2.62 mg/L) and yeast extract as a nitrogen source (465.21 ± 3.11 and 245.17 ± 3.24 mg/L), respectively. Carbon to nitrogen ratio for maximum EPS and IPS production was observed as 10 : 1 (395.29 ± 2.15 and 235.62 ± 1.40 mg/L), respectively. Glucose was found to be the major monosaccharide (62.15 ± 7.33%). Both EPS and IPS of C. gracilis showed significant DPPH radical scavenging activity, ABTS radical scavenging activity, reducing power, and iron chelating activity.

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Section: Resultssupporting

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture ofCordyceps gracilis(Grev.) Durieu & Mont.

Sharma

Gautam

Atri

2015

Evidence-Based Complementary and Alternative Medicine

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“…Exopolysaccharides (EPSs) as valuable metabolites have also been secreted in large quantities into the culture broth of A. biennis. Many studies reported that the EPSs harvested from fermentation broths of various fungi or mushrooms exhibit notable and excellent bioactivities, such as immunostimulatory, antitumor and antioxidant activities, and other pharmacological functions, thereby having great potential for applications in food, medicine and cosmetics (Cao et al 2014;Chen et al 2019;He et al 2012;Meng et al 2010). To the best of our knowledge, no or little information is available on the isolation, characterization and bioactivities of EPS produced from A. biennis in submerged fermentation.…”

Section: Introductionmentioning

confidence: 99%

Preliminary Characterization of Exopolysaccharides Produced by Abortiporus biennis in Submerged Fermentation

Zhang¹,

Liu²,

Li³

et al. 2019

JSM

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Abortiporus biennis is a rare edible and medicinal mushroom that is valuable in traditional Chinese medicine. Exopolysaccharides (EPSs) as valuable metabolites have been harvested from fermentation broths of A. biennis. However, studies on preliminary characterisations and bioactivities of A. biennis EPSs are lacking. In this study, therefore, the production, characteristics and bioactivity of EPSs produced by A. biennis in submerged fermentation were investigated. An EPS-1 was isolated and purified from the culture broth of A. biennis using a Sepharose CL-6B column chromatography. The molecular weight, monosaccharide composition, structural characteristics, chain conformation and thermal property of EPS-1 were determined by gel-filtration chromatography, gas chromatography-mass spectroscopy, Fourier-transform infrared spectroscopy, Congo red test and thermogravimetric analysis, respectively. The in vitro antioxidant and immunoregulation activities of the EPS-1 were evaluated by adopting DPPH and hydroxyl radical-scavenging assays, and analysing NO production in macrophage RAW 264.7 cells, respectively. Results showed that the culture medium containing 30 g/L lactose and 3.0 g/L tryptone had the most suitable carbon and nitrogen sources, respectively, for EPS production in A. biennis. The maximum EPS production (18.29 g/L) was obtained after 6 days of cultivation in a 5 L stirred-tank reactor under the most suitable culture medium. After purification, EPS-1 with 85.4% yield and 96.1% carbohydrate content was obtained from the culture broth of A. biennis. EPS-1 was characterised as a neutral heteropolysaccharide with a molecular weight of 22.07 kDa and is composed of glucose, mannose and galactose at a molar ratio of 3.3:2.0:1.0. EPS-1 with a relatively high thermal stability existed as a random coil conformation in the aqueous medium and possessed prominent radical-scavenging ability and macrophage stimulation activity in vitro. Therefore, EPS-1 could be explored as a functional ingredient with potential applications in food, medical and cosmetic industries.

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“…[10] Compared with the intracellular substances, the extracellular substances from fermentation broth with similar physiological and pharmacological functions are easily obtained. [11] In fact, food manufactures have directly employed EPS of mushrooms by fermentation to prepare drinks and capsules for sale. Recently, some studies showed that the compositions of the growth medium can affect the specific rate of EPS synthesis.…”

Section: Introductionmentioning

confidence: 99%

Optimization of fermentation media composition of ganoderma lucidum for improved production of antioxidant and antimicrobial compounds

Paliya¹,

Chaudhary²,

Verma³

et al. 2014

Phcog Commn.

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Optimization for the production of exopolysaccharides from Morchella esculenta SO-02 in submerged culture and its antioxidant activities in vitro

Cited by 49 publications

References 22 publications

Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture ofCordyceps gracilis(Grev.) Durieu & Mont.

Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture ofCordyceps gracilis(Grev.) Durieu & Mont.

Preliminary Characterization of Exopolysaccharides Produced by Abortiporus biennis in Submerged Fermentation

Optimization of fermentation media composition of ganoderma lucidum for improved production of antioxidant and antimicrobial compounds

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