Optimization of Acid Protease Production by<i>Aspergillus niger</i>I1 on Shrimp Peptone Using Statistical Experimental Design

Siala, Rayda; Frikha, Fakher; Mhamdi, Samiha; Nasri, Monçef; Kamoun, Amel

doi:10.1100/2012/564932

Cited by 53 publications

(42 citation statements)

References 49 publications

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“…29 For this study, six factors, including concentrations of HPA (A), ctMGAM (B), ntMGAM (C), starch (D), reaction pH (E) and temperature (F), were evaluated for their effect on starch digestion at two levels ( Table 1). A total of 15 experiments with were designed by Design-Expert software ( Table 2).…”

Section: Identification Of the Critical Factors Involved In Starch DImentioning

confidence: 99%

“…The main advantage of RSM is its ability to reduce the number of experimental trials needed to evaluate multiple parameters and their interactions, to obtain statistically acceptable results. 29,30 It has been successfully used to optimize process variables. 31,32 Although the individual roles of these catalytic units (HPA, ntMGAM and ctMGAM) are relatively well studied, the combinatorial roles of the HPA and MGAM complexes in starch digestion process have not been investigated in depth.…”

Section: Introductionmentioning

confidence: 99%

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Modeling of cooked starch digestion process using recombinant human pancreatic α-amylase and maltase-glucoamylase for in vitro evaluation of α-glucosidase inhibitors

Cao

Zhang

Dong

et al. 2015

Carbohydrate Research

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Section: Identification Of the Critical Factors Involved In Starch DImentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Modeling of cooked starch digestion process using recombinant human pancreatic α-amylase and maltase-glucoamylase for in vitro evaluation of α-glucosidase inhibitors

Cao

Zhang

Dong

et al. 2015

Carbohydrate Research

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“…Penambahan enzim alpha amilase juga meningkatkan total protein pada broiler dan mengurangi kandungan lemak (Ghosh et al, 2001). Fungi terutama Aspergillus niger, efektif menghasilkan fitase dan amilase (Siala et al, 2012;Sivaramakrishnan et al, 2007). Aspergillus niger (A. niger) dan Neurospora sitophila (N. sitophila) dipilih sebagai penghasil fitase dan amilase karena keduanya termasuk dalam kelompok generated as safe (GRAS) (Schuster et al, 2002;Davis dan Perkins, 2002).…”

Section: Pendahuluanunclassified

POTENSI DARI KAPANG Aspergilus niger, Rhizophus oryzae DAN Neurospora sitophila SEBAGAI PENGHASIL EZIM FITASE DAN AMILASE PADA SUBSTRATE AMPAS TAHU

Kanti

2017

BuletinPeternak

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INTISARIPenelitian ini bertujuan untuk mendapatkan kondisi optimal untuk produksi enzim amilase dan fitase pada media ampas tahu menggunakan Aspergillus niger (A. niger), Rhizophus oryzae (R. oryzae) dan Neurospora sitophila (N. sitophila) sebagai starter. Uji kemampuan produksi enzim fitase dan amilase oleh A. niger, R. oryzae dan N. sitophila dilakukan menggunakan media ampas tahu yang disterilisasi. Pemilihan ketiga isolat ini diawali dengan uji produksi enzim amilase pada kultur cair yang mengandung 2% pati, dan uji fitase dilakukan pada media yang mengandung 0,5% sodium fitat. Hasil uji pada medium cair selanjutnya digunakan untuk uji produksi enzim amilase dan fitase pada sistem fermentasi padat (SSF) menggunakan ampas tahu sebagai media fermentasi. Untuk mendapatkan produksi enzim yang tinggi dilakukan melalui optimasi waktu inkubasi, suhu inkubasi dan pH media. Fitase dan amilase dapat diproduksi dengan media ampas tahu oleh R. oryzae, A. niger dan N. sitophila. Kondisi optimum untuk produksi fitase, yaitu waktu inkubasi pada hari keempat untuk ketiga kapang, suhu 25°C untuk R. oryzae dan A. niger, suhu 30°C untuk N. sitophila, pH 8 untuk R. oryzae, pH 6 untuk A. niger dan N. Sitophila. Neurospora sitophila menghasilkan amilase optimum pada suhu 35°C, sedangkan A. niger dan R. oryzae optimum pada suhu 30°C. Aktivitas enzim amilase optimum adalah 162,23 U/g; 181,21 U/g dan 192,11 berturut turut oleh R. oryzae, A. niger dan N. Sitophila. Penurunan aktivitas produksi amilase menurun oleh R. oryzae pada suhu 40°C. Amilase diproduksi optimal pada pH 6-7. Aktivitas fitase yang dihasilkan oleh R. oryzae, A. niger dan N. sitophila, yaitu sebesar 51,32 U/g, 53,48 U/g, dan 60,54 U/g. Pakan ternak yang mengandung asam fitat mampu dihidrolisis oleh fitase pada kondisi optimum. Ketiga kapang juga menghasilkan enzim amilase pada media ampas tahu mengindikasikan bahwa ampas tahu merupakan susbtrat yang baik untuk produksi enzim hidrolisis yang berguna untuk meningkatkan nilai nutrisi pakan ternak.(Kata kunci: Amilase, Ampas tahu, Aspergillus niger, Neurospora sitophila, Phytase, Rhizopus oryzae) ABSTRACTThe objective of the study was to obtain the optimal condition to produce phytase and amylase enzyme using soybean curd residue with Aspergilus niger (A. niger) and Rhizopus oryzae (R. oryzae) and Neurospora sitophila (N. sitophila) as inoculants. These isolates were selected due to their ability to produce amylase in 2% of starch medium, and phytase in 0.5% of sodium phytase contain medium. Optimation of phytase and amylase production were performed on incubation time, pH, and temperature. Phytase and amylase were produced by these 3 isolates on soybean curd residue. Phytase production was achieved at 4 days incubation time. The optimal condition for phytase production varied depend on isolates. R. oryzae was optimal at pH 8, 25°C, while A. niger and N. sitophila were at pH 6.0-7.0 at 30°C. Amylase production was optimal at pH 6.0-7.0 after 3-4 days. Amylase production by R. oryzae was inhibited at 40°C. Application o...

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“…Increase in slurry concentration and pH, decreased the protease activity. Many extracellular proteases are active in acidic environments, and hence increase in pH decreases its activity (Siala et al, 2012;Chandrasekaran et al, 2015). Increase in slurry concentration decreased the enzyme activity as inoculum level is insufficient to utilize the increases slurry for enzyme production.…”

Section: Ph Verses Slurry Concentrationmentioning

confidence: 99%

POULTRY DROPPING BASED BIODIGESTED SLURRY FOR PROTEASE PRODUCTION BY Humicola fuscoatra MTCC 1409: OPTIMIZATION AND KINETIC STUDY

Goyal¹,

Dar²,

Phutela³

2018

JEBAS

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Protease is one of the most important groups of commercially produced enzymes. This study was aimed at the optimization and kinetics of protease production from poultry dropping based biodigested slurry by Humicola fuscoatra MTCC 1409. Four significant variables (pH, temperature, slurry concentration and inoculum concentration) were considered for optimization both by one variable at a time approach and response surface methodology. The maximum protease production in the poultry dropping based biodigested slurry was (531±1.37 U g -1 ) under the optimum conditions of pH (5), temperature (40°C), slurry concentration (25%) and inoculum concentration (10%). The protease production was found to be 3.38 fold higher under optimized conditions as compared to the non-optimized ones. The thermal inactivation of protease produced from biodigested slurry was investigated kinetically within temperature range of 30-70°C. The irreversible inactivation was well described by first order kinetics with k values increasing between 0.0028 to 0.0071 min -1 and t1/2 decreasing from247.70 to 98.10 mins.At higher temperature, there was significant decrease in residual activity. The activation energy, enthalpy, Gibbs free energy and entropy range calculated on the basis of residual activity experiments conducted at temperature range of 30-70°C was found to be 21.29, 18.44 to 18.78 kJ mol -1

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Optimization of Acid Protease Production byAspergillus nigerI1 on Shrimp Peptone Using Statistical Experimental Design

Cited by 53 publications

References 49 publications

Modeling of cooked starch digestion process using recombinant human pancreatic α-amylase and maltase-glucoamylase for in vitro evaluation of α-glucosidase inhibitors

Modeling of cooked starch digestion process using recombinant human pancreatic α-amylase and maltase-glucoamylase for in vitro evaluation of α-glucosidase inhibitors

POTENSI DARI KAPANG Aspergilus niger, Rhizophus oryzae DAN Neurospora sitophila SEBAGAI PENGHASIL EZIM FITASE DAN AMILASE PADA SUBSTRATE AMPAS TAHU

POULTRY DROPPING BASED BIODIGESTED SLURRY FOR PROTEASE PRODUCTION BY Humicola fuscoatra MTCC 1409: OPTIMIZATION AND KINETIC STUDY

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