Optimization of Algerian fir somatic embryos maturation

Vooková, B.; Machava, Ján; Šalgovičová, A.

doi:10.1007/s10535-010-0030-2

Cited by 7 publications

(3 citation statements)

References 21 publications

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“…Plant regeneration systems developed for conifer species typically employ embryonic tissues as starting material (Stange et al 1999, Zhang et al 2006, Vooková et al 2010. Optimization of factors influencing each step of the micropropagation procedure is necessary for successful regeneration through adventitious bud induction (Kaul 1990).…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Micropropagation of Pinus peuce

Stojičić¹,

Janošević²,

Uzelac³

et al. 2012

Biologia plant.

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In Pinus peuce zygotic embryo culture grown on Gresshoff and Doy (1972; GD) basal medium, 2.22 µM benzyladenine (BA) was superior in promoting adventitious bud induction during 4 weeks comparing to kinetin or BA + kinetin. Shoot elongation was achieved on half-strength GD medium devoid of plant growth regulators and containing activated charcoal. Pulse treatment with 1 mM indole-3-butyric acid (IBA) for 2 h, followed by transfer to half-strength GD medium, produced the most efficient rooting. Rooted shoots were transplanted to the greenhouse and plantlets continued to grow and developed into phenotypically normal plants. Up to 10 plants per explant can be obtained within 36 weeks from culture initiation.

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Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Micropropagation of Pinus peuce

Stojičić¹,

Janošević²,

Uzelac³

et al. 2012

Biologia plant.

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“…However, when considering the potential for the application of the approach to other conifers the demand for plant growth regulators in the cultivation protocol can differ. The regulation of somatic embryogenesis of firs is even more complicated: investigations of somatic embryogenesis in species and hybrids such as A. alba, A. alba x numidica, Abies balsamea, A. concolor, A. cephalonica, A. fraseri, A. lasiocarpa, A. nordmanniana and A. numidica have been made [10][11][12][13][14][15][16][17][18][19][20][21]. Usually cytokinin(s) alone are able to trigger the process of induction of ESM in fir.…”

Section: Introductionmentioning

confidence: 99%

The role of auxins in somatic embryogenesis of Abies alba

et al. 2011

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The somatic embryogenesis of conifers is a process susceptible to exogenous phytohormonal treatments. We report the effects of the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the auxin inhibitor p-chlorophenoxyisobutyric acid (PCIB) on the endogenous level of the auxin indole-3-acetic acid (IAA) and on the anatomical composition of early somatic embryos of Abies alba (European silver fir). The embryogenic suspensor mass (ESM) of Abies alba proliferated on a medium supplemented by 2,4-D as well as on an auxin-free medium. The endogenous level of IAA was significantly higher in the ESM cultivated on a medium supplemented by 2,4-D. The decrease in the endogenous level of IAA in the first week of maturation is one of the most important stimuli responsible for the subsequent development of embryos. However, suppression of IAA synthesis by an auxin inhibitor did not stimulate the development of embryos. The maturation of somatic embryos from the globular to the cotyledonary stage occurs when the concentration of endogenous auxin in the ESM (including the embryos) increases. Early somatic embryos proliferating on a medium supplemented by auxin had an increased probability of maturing successfully. Exogenous auxin treatment during maturation did not compensate for the auxin deficiency during proliferation.

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“…Unlike other genera in the Pinaceae, Abies requires only cytokinin for induction of embryogenic tissue from zygotic embryos (Schuller et al 1989;Nørgaard and Krogstrup 1991), although the embryogenic suspensor mass of A. alba proliferated on a medium supplemented by 2,4-D as well as on an auxin-free medium (Vondrák-ová et al 2011). To improve maturation process, the most beneficial was pre-culturing of embryogenic tissue on SH medium (Schenk and Hildebrandt 1972) without growth regulator for 2 weeks (Vooková et al 2010). Maturation of fir somatic embryos is promoted by abscisic acid (ABA) and the presence of carbohydrates in the maturation medium.…”

Section: Process Of Somatic Embryogenesis In Abiesmentioning

confidence: 99%

Study of Abies somatic embryogenesis and its application

Vooková

2013

Dendrobiology

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This paper provides results of somatic embryogenesis study in our laboratory. General description of somatic embryogenesis (SE) induction, maturation of somatic embryos and plantlets regeneration of the Abies species, followed by a comparisons of some characteristics of zygotic and somatic embryos, seedlings and emblings (somatic seedlings). Own results are supplemented with some literature data. Also aplication of SE for improving of plantlet regeneration of elite fir trees from Dobroč primeval is described as well as initiation of the SE from seeds of incompatible crossings of firs where zygotic embryos abort usually several weeks after pollination.

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Optimization of Algerian fir somatic embryos maturation

Cited by 7 publications

References 21 publications

Micropropagation of Pinus peuce

Micropropagation of Pinus peuce

The role of auxins in somatic embryogenesis of Abies alba

Study of Abies somatic embryogenesis and its application

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