Optimization of alkaline extraction of hemicellulose from sweet sorghum bagasse and its direct application for the production of acidic xylooligosaccharides by Bacillus subtilis strain MR44

Abstract

As predominant components of hemicelluloses in grasses, methylglucuroarabinoxylans (MeGAXn) are sources for the production of acidic xylooligosaccharides (U-XOS). Bacillus subtilis MR44, an engineered biocatalyst to secrete only the XynC xylanase and Axh43 arabinoxylan hydrolase is capable of processing MeGAXn to exclusively U-XOS. The present studies are directed at the explosion on direct alkaline extraction serving for production of U-XOS. Response Surface Methodology was used to optimize xylan extraction conditions on the sweet sorghum bagasse to achieve maximum hemicelluloses yield. The optimized condition was as follows: extraction time of 3.91 h, extraction temperature of 86.1°C, and NaOH concentration (w/w) of 12.33%. Crude xylan extracted with NaOH revealed a compositional analysis of xylose (79.0%), arabinose (5.3%), glucose (1.7%), lignin and ash (5.6%). After neutralization this xylan preparation supported growth of MR44, processing MeGAXn from sweet sorghum and accumulating U-XOS. The quality of U-XOS produced by MR44 using alkaline-treated sweet sorghum bagasse was comparable to that obtained from purified MeGAXn. Overall, the present study demonstrates that direct alkaline treatment of sweet sorghum bagasse is useful to improve the bioavailability of MeGAXn for MR44-mediated conversion to U-XOS with average degrees of polymerization of 11–12, providing alternative resources with applications in nutrition and human and veterinary medicine.

Alkaline organosolv pretreatment of different sorghum stem parts for enhancing the total reducing sugar yields and p-coumaric acid release

“…Sorghum is one of the main bioenergy crops, and the biorefinery of its soluble sugar and bagasse residue for biofuels and biochemicals has attracted much attention in recent years [17][18][19][20]. Beside soluble sugar and lignocellulose residue, sorghum stem also contains significant amounts of hydroxycinnamic acid, such as p-coumaric acid (pCA, up to 35 mg/g cell walls), which is far higher than other agricultural waste biomass such as rice straw (6.8 mg/g cell walls) [21,22].…”

Alkaline organosolv pretreatment of different sorghum stem parts for enhancing the total reducing sugar yields and p-coumaric acid release

“…Sorghum is one of the main bioenergy crops, the biorefinery of its soluble sugar and bagasse residue for biofuels and biochemicals has attracted much attention in recent years [17][18][19][20]. Beside soluble sugar and lignocellulose residue, sorghum stem also contains significant amounts of hydroxycinnamic acid, such as p-coumaric acid (pCA, up to 35 mg/g cell walls), which is far higher than other agricultural waste biomass such as rice straw (6.8 mg/g cell walls) [21,22].…”

Alkaline organosolv pretreatment of different sorghum stem parts for enhancing the total reducing sugar yields and p-coumaric acid release

“…Sorghum is one of the main bioenergy crops, the biore nery of its soluble sugar and bagasse residue for biofuels and biochemicals has attracted much attention in recent years [17][18][19][20]. Beside soluble sugar and lignocellulose residue, sorghum stem also contains signi cant amounts of hydroxycinnamic acid, such as p-coumaric acid (pCA, up to 35 mg/g cell walls), which is far higher than other agricultural waste biomass such as rice straw (6.8 mg/g cell walls) [21,22].…”