Optimization of Amylase Production fromB. amyloliquefaciens(MTCC 1270) Using Solid State Fermentation

Abstract

Demand for microbial amylase production persists because of its immense importance in wide spectrum industries. The present work has been initiated with a goal of optimization of solid state fermentation condition for amylase using agroindustrial waste and microbial strain like B. amyloliquefaciens (MTCC 1270). In an aim to improve the productivity of amylase, fermentation has been carried out in the presence of calcium (Ca+2), Nitrate (NO3 −), and chloride ions (Cl−) as well as in the presence of D-inositol and mannitol. Amylase needs calcium ion for the preservation of its structure, activity and stability that proves beneficial also for amylase production using solid state fermentation. The inclusion of ions and sugars in the SSF media is promising which can be explained by the protection offered by them against thermal decay of amylase at various incubation periods at 37°C.

Optimization of the Medium for the Production of Extracellular Amylase by thePseudomonas stutzeriISL B5 Isolated from Municipal Solid Waste

“…The crystal structure of amylase showed that calcium ions play a detrimental role in ionic interaction with Asn 100 and His 201 residues of domain A and also with Asp 159 and Asp 167 residues of domain B in amylase enzyme. It has also been reported that the active site of amylase is located between domain A and domain B and calcium promotes stability and catalytic activity of the enzyme by interconnecting these two domains [36, 37]. …”

Effect of antibiotic on survival and development ofSpodoptera litura(Lepidoptera: Noctuidae) and its gut microbial diversity

“…Certain microbiota may be responsible for the production of digestive enzymes in some insects (Terra et al, 1996). M. paraoxydans, B. methylotrophicus and B. amyloliquefaciens isolated from S. litura larvae have already been known to produce digestive enzymes (Madhaiyan et al, 2010;Ojha et al, 2013;Saha et al, 2014). Higher abundance of these bacteria on S+ diet in comparison with S− diet may have resulted in increased level of digestive enzymes in larvae feeding on S+ diet.…”

Acid stable α-amylase from Pseudomonas balearica VITPS19—Production, purification and characterization

“…For the extracellular amylase production, the composition and concentration of various components of media greatly influence the growth and production of amylase [ 12 ]. Being a metalloenzyme, Ca 2+ is required for its integrity, activity and structural stabilization [ 13 ]. So, in order to yield maximum amylase production, media components and environmental conditions should be optimized.…”