Highlights
α – Amylase was produced from a rhizobacteria
Pseudomonas balearica
VITPS19.
One factor at a time method (OFAT) was employed to optimize the α –amylase production.
Three step purification of α – amylase from the fermentation broth.
Determining the optimal conditions for enzyme activity.
Estimation of the enzymatic kinetic parameters of the α-amylase.
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