Optimization of Antibacterial Production of Endophytic Fungi with Various Sources of C, N, and pH using The Response Surface Methodology

Widjajanti, Hary; Nurnawati, Elisa; Muharni, Muharni; Zahwa, Eca Desriana

doi:10.26554/sti.2022.7.2.149-157

Cited by 5 publications

(4 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Penggunaan medium MYB karena media ini memiliki kandungan sumber nitrogen seperti pepton, ekstrak ragi dan sumber karbon seperti maltosa yang memberikan kondisi optimal untuk pertumbuhan fungi dalam fermentasi 29…”

Section: Ifaz-6 Tidak Bersekat Adaunclassified

Potential of Endophyctic Fungi Isolates IFAZ-6 from Bidara Root (Ziziphus mauritiana Lam.) as Antibacterials Againts Digestive Tract Infection

Janna,

Fitriana,

Amirah

2024

j. microbiol. sci

View full text Add to dashboard Cite

Bidara root (Ziziphus mauritiana Lam.) has activity as an antibacterial against digestive tract infections. This study aims to determine the antibacterial activity of isolate IFAZ-6 endophytic fungi of bidara root against bacteria that cause digestive tract infections using the TLC-Bioautography method. Purification was conducted on the active isolate IFAZ-6 to obtain a pure endophytic fungi isolate. Subsequently, macroscopic and microscopic observations were carried out. Then, fermentation was performed in MYB (Maltose Yeast Broth) medium for 21 days. Screening and evaporation were conducted to produce an extract. The obtained extract was subjected to TLC analysis using a chloroform: methanol eluent (7:1). The TLC-Bioautography test was conducted against Escherichia coli, Salmonella typhi, Shigella dysentriae, and Vibrio cholerae bacteria. The TLC identification results showed one spot with an Rf value of 0.80 for the IFAZ-6 isolate. The chromatogram profile obtained from the TLC-Bioautography method for the IFAZ-6 isolate demonstrated antibacterial activity against bacteria that cause digestive tract infections.

show abstract

Section: Ifaz-6 Tidak Bersekat Adaunclassified

Potential of Endophyctic Fungi Isolates IFAZ-6 from Bidara Root (Ziziphus mauritiana Lam.) as Antibacterials Againts Digestive Tract Infection

Janna,

Fitriana,

Amirah

2024

j. microbiol. sci

View full text Add to dashboard Cite

show abstract

“…The inoculants were incubated in room settings for 3 to 14 days. Transferring the colonies to a fresh medium in petridish and culturing them there for 48 hours will purify the endophytic fungus (Khalil et al, 2021;Talukdar et al, 2021;Widjajanti et al, 2022).…”

Section: Procedures 231 Processing Of the Sample And Isolation Of End...mentioning

confidence: 99%

Antioxidant and Antibacterial Activity of Endophytic Fungi Isolated from Fruit of Sungkai (Peronema canescens)

Oktiansyah,

Widjajanti,

Setiawan

et al. 2024

Sci. Technol. Indones

View full text Add to dashboard Cite

Peronema canescens, often known as sungkai, is widely used and can be found all around Indonesia. The public believes that the leaves may reduce fever and strengthen the immune system. However, the effectiveness of sungkai fruit has not been thoroughly investigated. In this research, we looked at endophytic fungus extracts from sungkai fruit’s that have antioxidant and antibacterial properties. The study’s results will serve as the foundation for further investigation into the development of potential natural chemicals with antioxidant and antibacterial properties. Morphologically, the endophytic fungi isolated from sungkai fruit were identified. The antioxidant and antibacterial properties of endophytic fungal extracts were studied using the DPPH technique and the paper disk diffusion method. By employing molecular identification and column chromatography to separate the active compounds, the most likely endophytic fungal isolates were found based on the results of the bioactivity tests. Using 1D NMR spectroscopic methods, the chemical’s structure was determined, and the results were compared to NMR data for the same compound published in the literature. Fruit of sungkai had 8 strains of endophytic fungus (RBH1-RBH8). Strong antibacterial and very strong antioxidant activity were shown by the RBH5 isolate (IC50 < 20 μg/mL). Pythium periplocum was determined to be the RBH5 isolate based on molecular testing. Pure chemical compound extracted from RBH5 isolates shown highly potent and potent antibacterial and antioxidant effects. The chemical compound was identified by spectroscopy as 3-hydroxy-4(hydroxy(4-hydroxyphenyl)methyl)-γ-butyrolactone. The results of this study serve as the foundation for developing compounds as pharmaceutical raw materials via further research phases.

show abstract

“…Observing hyphae, spores, color, and other distinct traits under a microscope with a 1000X magnification is used to analyze microscopic characteristics using the slide culture method. The characters that emerge were compared to the solitary characters tested in 2022 (Pitt and Hocking, 2009;Walsh et al, 2018;Watanabe, 1993).…”

Section: Research Procedures 231 Endophytic Fungi Rejuvenation and Mo...mentioning

confidence: 99%

Combination Effect of Extracts and Pure Compounds of Endophytic Fungi Isolated from Sungkai (Peronema canescens) Leaves on Antioxidant Activity

Elfita,

Oktiansyah,

Mardiyanto

et al. 2024

Sci. Technol. Indones

View full text Add to dashboard Cite

Endophytic fungi extracts have various biological and pharmacological activities as natural antioxidants which have the potential to be developed in the pharmaceutical. Drugs made from natural substances frequently work best when they are present in large quantities. This may result in unfavorable side effects from using more of one substance at a time. This limitation can be overcome by using a combination of the extracts/pure compounds that allow synergistic interactions with strong antioxidant properties at fairly low concentrations. The purpose of this research was to determine the combined effect of endophytic fungi extracts and its pure compounds on their antioxidant activity. This research was initiated by rejuvenating eight selected endophytic fungal isolates from Sungkai leaves and morphological characterization was carried out. Each fungus was cultivated in PDB medium for 4 weeks under statistical conditions. Ethyl acetate was used to extract the endophytic fungi’s liquid culture, which was then evaporated. Each endophytic fungal extract (PD1-PD8) and their five pure compounds were tested for antioxidant activity by the DPPH method. Then a combination of two and three extracts was carried out with a ratio of 1:1. The results show that the interaction of the mixture of endophytic fungi extracts and their pure compounds can be classified as synergistic (combined effect of the extracts > individual effects), additive (combined effect of the extracts = individual effect), and nothing is antagonistic (combined effect of the extracts < individual effects). The best combination in this study was PD4+PD5+PD6 (1:1:1) which could be used as a formula for further research into in vivo immunostimulant tests. Another conclusion from this research is that the pure compounds contained in the extracts have lower antioxidant activity than the extracts and do not have a synergistic effect on the combination.

show abstract

Optimization of Antibacterial Production of Endophytic Fungi with Various Sources of C, N, and pH using The Response Surface Methodology

Cited by 5 publications

References 19 publications

Potential of Endophyctic Fungi Isolates IFAZ-6 from Bidara Root (Ziziphus mauritiana Lam.) as Antibacterials Againts Digestive Tract Infection

Potential of Endophyctic Fungi Isolates IFAZ-6 from Bidara Root (Ziziphus mauritiana Lam.) as Antibacterials Againts Digestive Tract Infection

Antioxidant and Antibacterial Activity of Endophytic Fungi Isolated from Fruit of Sungkai (Peronema canescens)

Combination Effect of Extracts and Pure Compounds of Endophytic Fungi Isolated from Sungkai (Peronema canescens) Leaves on Antioxidant Activity

Contact Info

Product

Resources

About