Optimization of aqueous-assisted extraction of polysaccharides from pumpkin (Cucurbita moschata Duch) and their biological activities

Abstract

The pumpkin pulp contains a greater composition of edible polysaccharides and has reported with excellent biological applications. This research pertains to optimize the extraction of polysaccharides from the fleshy portion of the pumpkin using aqueous assisted extraction (AAE). The result showed that the optimal extraction condition of pumpkin polysaccharide was as follows: extraction temperature at 55 °C, pH 4.5, and enzyme concentration of 4000 µ/g for 80 min. Under the optimal extraction condition, the yield of pumpkin polysaccharide via AAE (15.4) was significantly higher. The biological activities of extracted polysaccharide including α-amylase inhibition (57.41% at 1000 µg/mL) and anti-inflammatory (50.41% at 25 µg/mL) activity increased significantly. Additionally, the antioxidant activities of extracted pumpkin polysaccharides including IC 50 values of DPPH and ABTS were 59.87% and 58.74%, respectively. The pumpkin polysaccharide has maximum inhibitory effects against bacterial strains especially for Escherichia coli than that of fungal strains. It is suggested that the aqueous assisted extraction of is a cost-effective promising method to decrease the processing time as well as enhancing extracted polysaccharide yield – times.

Rheological and Microstructural Properties of Acidified Milk Drink Stabilized with Butternut Squash Pulp Hydrocolloids (BSPHs)

“…BSPHs present values of 4.80 ± 0.63% moisture, 3.01 ± 0.53% ash, 1.10 ± 0.35% fat, 12.21 ± 0.11% protein, and 79.97 ± 0.240% carbohydrate, associated with saccharide content including xylose, arabinose, glucose, galactose, and gluconic acids, the main components of Cucurbita sp. polysaccharides . The results demonstrate that the pulp of butternut squash (C.…”

“…polysaccharides. 30 The results demonstrate that the pulp of butternut squash ( C. moschata ) is an excellent source of carbohydrates for the commercial production of hydrocolloids.…”

“…However, there are few reports on squash pulp characterization and techno-functional properties and their use in developing food products. For example, Umavathi et al 26 extracted polysaccharides from C. moschata Duch with biological activities (anti-inflammatory, antioxidant, and antimicrobial activities). Du et al 27 studied the prebiotic potential of oligosaccharides from C. moschata with high solubility.…”

Influence of extraction methods on navel orange peel pectin: structural characteristics, antioxidant activity and cytoprotective capacity

“…Hydrothermal treatment, a pseudo‐acid extraction technique, generates hydronium ions from in situ generated acids or water auto‐ionisation under elevated temperatures and pressures (Gullon et al ., 2012). Considering the mechanism, a possible reason behind hydrothermal extraction's low yield could be excessive hydrolysis of polysaccharide at high temperatures (Umavathi et al ., 2021).…”

An acidic polysaccharide from Oxalis corniculata L. and the preliminary study on its antioxidant activity

“…The OCP yield increased with temperature but decreased when the temperature exceeded 50°C (Figure 1a). High temperature might promote intermolecular thermal motion to increase the extraction speed but target substances might be severely damaged by excessive temperature exposure (Umavathi et al, 2021). Therefore, temperature of 50°C was chosen for optimization.…”