Optimization of biomass and hydrogen production by Anabaena sp. (UTEX 1448) in nitrogen-deprived cultures

Vargas, Sarah Regina; Santos, Paulo Vagner dos; Zaiat, Marcelo; Calijuri, Maria do Carmo

doi:10.1016/j.biombioe.2018.01.022

Cited by 53 publications

(12 citation statements)

References 37 publications

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“…Thus, a higher amount of biomass was obtained in the rst step at a lower pH (6.2) and light intensity of 200 µmol m − 2 s − 1 . It is believed that this biomass optimization provided the inoculation of a greater amount of biomass in the photobioreactors and favored the hydrogen production variable test as studies have reported that an increased amount of biomass can improve the hydrogen production yield [6][7][8]21].…”

Section: Discussionmentioning

confidence: 99%

Influence of Sulfur and Light Intensity in Nutrient Removal, and Hydrogen and Ethanol Production by Optimized Biomass of Chlamydomonas Reinhardtii in Batch Anaerobic Photobioreactors

Vargas

Zaiat

Calijuri

2021

Preprint

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Hydrogen is a renewable fuel that can be biologically produced by green algae in closed anaerobic photobioreactors with light and organic carbon as energy sources. This research aimed to investigate the influence of sulfur concentration and light intensity on hydrogen and ethanol production, as well as on nutrient removal by Chlamydomonas reinhardtii (CC425) in batch cultures. The strain was cultivated in two phases: in the first step, the cultures were maintained under aerobic conditions to obtain biomass; in the second step, the biomass was transferred to closed anaerobic photobioreactors for gas generation under continuous illumination. A factorial design was accomplished to optimize the biomass production in the first step, with light variation, pH, and glucose addition. Afterward, light intensity and sulfur concentration were varied to test hydrogen production in the second step. The best production occurred in photobioreactors without sulfur addition (average increase of 7 times in the production) and under higher light intensity the production was 37% higher than lower light (39.64 ± 2.44 µmol H2 L-1 h-1). There was an effect of sulfur concentration in the ethanol production and under higher light intensity the production was higher (203.20 ± 31.49 mg L-1). In addition, in general, under conditions with the presence of sulfur, there was greater removal of ammoniacal nitrogen (5.3%), phosphate (85.0%), COD (9.1%) and acetic acid (97.2%). This research demonstrates the efficient production of hydrogen and ethanol by C. reinhardtii and it shows that the process can be associated with nutrient removal.

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Section: Discussionmentioning

confidence: 99%

Influence of Sulfur and Light Intensity in Nutrient Removal, and Hydrogen and Ethanol Production by Optimized Biomass of Chlamydomonas Reinhardtii in Batch Anaerobic Photobioreactors

Vargas

Zaiat

Calijuri

2021

Preprint

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“…The increment of photobiological production of hydrogen is related to the carbon content in biomass [156,157,158]. Other strains of interest are Anabaena able to produce hydrogen [159]. Additionally, S. platensis can produce hydrogen in dark conditions with photobiological hydrogen production [157].…”

Section: Current Applicationsmentioning

confidence: 99%

Mexican Microalgae Biodiversity and State-Of-The-Art Extraction Strategies to Meet Sustainable Circular Economy Challenges: High-Value Compounds and Their Applied Perspectives

et al. 2019

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In recent years, the demand for naturally derived products has hiked with enormous pressure to propose or develop state-of-the-art strategies to meet sustainable circular economy challenges. Microalgae possess the flexibility to produce a variety of high-value products of industrial interests. From pigments such as phycobilins or lutein to phycotoxins and several polyunsaturated fatty acids (PUFAs), microalgae have the potential to become the primary producers for the pharmaceutical, food, and agronomical industries. Also, microalgae require minimal resources to grow due to their autotrophic nature or by consuming waste matter, while allowing for the extraction of several valuable side products such as hydrogen gas and biodiesel in a single process, following a biorefinery agenda. From a Mexican microalgae biodiversity perspective, more than 70 different local species have been characterized and isolated, whereas, only a minimal amount has been explored to produce commercially valuable products, thus ignoring their potential as a locally available resource. In this paper, we discuss the microalgae diversity present in Mexico with their current applications and potential, while expanding on their future applications in bioengineering along with other industrial sectors. In conclusion, the use of available microalgae to produce biochemically revenuable products currently represents an untapped potential that could lead to the solution of several problems through green technologies. As such, if the social, industrial and research communities collaborate to strive towards a greener economy by preserving the existing biodiversity and optimizing the use of the currently available resources, the enrichment of our society and the solution to several environmental problems could be attained.

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“…It is well-known that the effect of light intensity may be different in various strains of cyanobacteria [10]. For example, low-light intensity was recommended for laboratory H 2 production by some filamentous cyanobacteria [29,30]. Besides, this effect may also depend on other factors: the cell concentration, duration of illumination and range of the light intensities.…”

Section: The Hydrogen Production In Experiments #2mentioning

confidence: 99%

Plastic bags as simple photobioreactors for cyanobacterial hydrogen production outdoors in Moscow region

Shastik

Romanova

Laurinavichene

et al. 2019

Int J Energy Environ Eng

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The application of transparent plastic bags with a gas barrier layer as inexpensive photobioreactors for H 2 production by the cyanobacterium Anabaena sp. PCC 7120 ∆Hup mutant cells was explored outdoors in Moscow region for the first time. Two experiments were performed in July and August, the average daily temperature being 21.7 and 20.6 °C, the average daily light intensity being 290 and 340 µmol photon m −2 s −1 in experiment #1 and #2, respectively. The maximal H 2 production rate was 20.6 mL day −1 L −1 of culture, with accumulation of 33.2 mL L −1 during 5 days and a final H 2 content of 1.1% (v/v). Molecular nitrogen added to the Ar gas at 3.3% significantly affected neither the rate nor the duration of H 2 production. Low morning temperatures as well as high daytime light intensities (especially at low cell concentrations) seemed to reduce the H 2 production rate. The activities obtained were lower as compared to the previously reported data. It could be attributable to suboptimal weather conditions and simple device arrangement. However, results prove that H 2 production by cyanobacteria is still feasible outdoors in plastic bags, the cheapest photobioreactors.

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Optimization of biomass and hydrogen production by Anabaena sp. (UTEX 1448) in nitrogen-deprived cultures

Cited by 53 publications

References 37 publications

Influence of Sulfur and Light Intensity in Nutrient Removal, and Hydrogen and Ethanol Production by Optimized Biomass of Chlamydomonas Reinhardtii in Batch Anaerobic Photobioreactors

Influence of Sulfur and Light Intensity in Nutrient Removal, and Hydrogen and Ethanol Production by Optimized Biomass of Chlamydomonas Reinhardtii in Batch Anaerobic Photobioreactors

Mexican Microalgae Biodiversity and State-Of-The-Art Extraction Strategies to Meet Sustainable Circular Economy Challenges: High-Value Compounds and Their Applied Perspectives

Plastic bags as simple photobioreactors for cyanobacterial hydrogen production outdoors in Moscow region

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