Optimization of biosurfactant production by Bacillus brevis using response surface methodology

Abstract

The present study aims to evaluate and validate a statistical model for maximizing biosurfactant productivity by Bacillus brevis using response surface methodology. In this respect, twenty bacterial isolates were screened for biosurfactant production using hemolytic activity, oil spreading technique, and emulsification index (E24). The most potent biosurfactant-producing bacterium (B. brevis) was used for construction of the statistical response surface model. The optimum conditions for biosurfactant production by B. brevis were: 33 °C incubation temperature at pH 8 for 10 days incubation period and 8.5 g/L glucose concentration as a sole carbon source. The produced biosurfactant (BS) (73%) exhibited foaming activity, thermal stability in the range 30–80 °C for 30 min., pH stability, from 4 to 9 and antimicrobial activity against (Escherichia coli). The BS gave a good potential application as an emulsifier.

Statistical Design, a Powerful Tool for Optimizing Biosurfactant Production: A Review

“…The effect of temperature, pH, incubation period, and glucose concentration were evaluated [44]. Emulsion reached 73%.…”

Optimization Techniques and Development of Neural Models Applied in Biosurfactant Production by <i>Bacillus subtilis</i> Using Alternative Substrates

“…According to described by [16] oil spreading was evaluated by adding 20 mL distilled water on a Petri dish followed by addition of 50 µL of oil to its surface.…”

Effects of Glucose and Ferrous Supplements and Culture Conditions on Lipopeptide Biosurfactant from Pseudomonas spp.

“…According to their compositions, surface tension is able to be decreased by surfactants. These compounds play many rolls of human life in many fields not only agricultural, cosmetic, pharmaceutical applications, but also in food industries [1]. However, the synthetic chemical surfactants are toxic and difficult to degrade [2].…”

“…Mouafi et al [1] reported that lipopeptide biosurfactant production from Bacillus brevis was increased by glucose supplementation in medium. The lipopeptide biosurfactant yield of Bacillus subtilis B20 was increased to 2.29 g/L when cultured with molasses [5].…”

“…In addition, biosurfactants exhibit important properties such as antimicrobial activity, anti-adhesion of microorganisms, biodegradability, emulsifying agents, and low toxicity. Therefore, biosurfactants have potentially applied in environmental, food industrial, agricultural, therapeutic and biomedical applications [1], [4].…”