Optimization of Burkholderia sp. ZYB002 lipase production for pitch control in thermomechanical pulping (TMP) processes

Abstract

Production and process optimization of a thermostable lipase from Burkholderia sp. ZYB002 and the application potential of this lipase for pitch control of thermomechanical pulp (TMP) has been investigated. The optimized physical and nutritional factors of Burkholderia sp. ZYB002 were found to be (all units in%, w v -1 ): 0.5 peptone, 0.025 urea, 0.5 glycerol, 0.1 NaHCO 3 , 0.2 K 2 HPO 4 , 1.5 Tween 80, 1.1 olive oil, four inoculum volume, and initial pH 8.5. The lipase production process was carried out at 308C at 250 rpm for 36 h in 250 ml Erlenmeyer flasks containing 35 ml fermentation medium and five glass beads. Under the optimized conditions, lipase activity reached 216.5 U ml -1 . In a TMP of Pinus massoniana, 39% of triglycerides were hydrolyzed by the lipase under optimized conditions wpulp concentration 6% (w v -1 ), lipase dose 20 U g -1 based on dry TMP, pulp initial pH 7.5, and 658C for 30 minx. The lipase in question displayed a great potential for pitch control of TMP.

Classification of lipolytic enzymes and their biotechnological applications in the pulping industry

“…Microbial lipases specifically have unlimited potential in commercial applications, such as additives in fine chemicals, wastewater treatment, food processing, cosmetics, detergents, pharmaceuticals, degreasing formulations, paper manufacture, and accelerated degradation of fatty wastes and polyurethane (Rodriques et al 2016;Shu et al 2016;Speranza et al 2016;Fulton et al 2015;Adulkar and Rathod 2014;Gerits et al 2014;Li et al 2014;Saranya et al 2014;Brabcova et al 2013;Lailaja and Chandrasekaran 2013;Nerurkar et al 2013;Whangsuk et al 2013;Zhang et al 2013;Liu et al 2012;Kamini et al 2000). Even though a large number of lipases have been described in the literature, only for a limited number of enzymes belonging to a few species has it been proven that their stability and biosynthetic activity is amenable for use in organic solvents, and thus for consideration as industrially applicable enzymes (Cardenas et al 2001;Jaeger et al 1999).…”

Cell-bound lipases from Burkholderia sp. ZYB002: gene sequence analysis, expression, enzymatic characterization, and 3D structural model

“…In previous research, Burkholderia sp. ZYB002 produced both extracellular lipase and cell-bound lipase [ 10 , 11 ]. The cell-bound lipase from Burkholderia cepacia displayed excellent interesterification activity for biodiesel production and highly enantioselective hydrolysis activity for L-menthol synthesis [ 12 , 13 ].…”