Optimization of Cellulase Producing<i>Aspergillus flavus</i>SB4 by Solid State Fermentation using Response Surface Methodology (RSM)-CCD

N, Utharalakshmi; Kumar, Anil; Narendrakumar, G.

doi:10.5958/0974-360x.2015.00058.x

Cited by 13 publications

(8 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the proposed optimal conditions differed between studies due to different sources of isolates (water, soil, decaying plants) and inherent production capacity of the isolates. Among the different types of fungi, A. flavus was favored for cellulase production (Gomathi et al, 2012;Utharalakshmi et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Expression of genes encoding cellulolytic enzymes in some Aspergillus species

et al. 2016

View full text Add to dashboard Cite

ABSTRACT. Fermentation is an important industrial process for microbial metabolite development and has wide applications in various fields. Aspergillus is the most important genus of fungi used for the production of microbial enzymes such as cellulase. The Aspergillus genome encodes various cellulolytic enzymes. In this study, we assayed the gene expression and cellulolytic enzyme production of three isolates: A. niger (KSU009), A. terreus (KC462061), and A. flavus (KSU014). Two fermentation systems, submerged fermentation and biofilm fermentation (BF), were used for this purpose. Gene expression analysis by RT-PCR showed that cbhB, exo, eglA, eglB, eglC, and β-actin genes were differentially expressed in the two fermentation systems for these three isolates during enzyme production. Furthermore, the expression of all genes was found to be higher in the BF system. The six genes were not expressed in the isolates with no cellulolytic enzyme production. The isolates were identified by morphological and molecular methods, which were based on macroscopic characteristics and sequence analysis of ITS1, ITS2, and the 5.8S regions of rDNA.

show abstract

Section: Discussionmentioning

confidence: 99%

Expression of genes encoding cellulolytic enzymes in some Aspergillus species

et al. 2016

View full text Add to dashboard Cite

show abstract

“…3 clearly show that the optimum pH for maximum production of cellulase. It was observed that the activity decreased with more increase in pH which indicates reduction in the activity [26,27].…”

Section: Effect Of Ph On Cellulase Activity (Filter Paper and Endoglumentioning

confidence: 97%

Analysis of Gut Flora From Damp Wood Termites (Trinervitermes Spp.) and Extraction, Characterization of Cellulase From the Isolate

Narendrakumar

Nmd

Prakash

et al. 2017

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Objective: The objective of the study was to isolate, characterize, identify, and compare the potentials of cellulolytic strains isolated from the gut of damp wood termites (Trinervitermes species) collected from Chennai, Tamil Nadu, India.Methods: Termites were collected and used as hoard of cellulase producers and screened for the cellulase producers using carboxymethyl cellulose as the sole source of carbon and were authenticated using Congo red plate screening method. The isolates showing a significant zone of clearance were further confirmed by biochemically characterized.Results: Nine effective isolates were characterized and three strained were used for analysis. The organisms were subjected with substrate, temperature (25°C, 37°C, and 45°C), and pH to optimize the cultural condition. The enzyme activity was estimated using endoglucanase, FPase after incubating at appropriate conditions. Five isolates showing a significant zone of clearance were selected, out of which three belonged to Bacillus and one each to Staphylococcus. Optimization of media and genetic modification of the strains can further convalesce their competence. All the isolates have ensuring application in view of use in future.Conclusion: An effective strain of bacteria was isolated from the gut of termites can be used as a potential candidate for the production of cellulase in industries.

show abstract

“…The correlation between the coded values of the input variables and the actual values was defined in equation described elsewhere [28][29][30][31][32][33][34][35] . The backslide information was introduced to backslide examination to assess backslide coefficients.…”

Section: Response Surface Optimizationmentioning

confidence: 99%

“…Surfactants are thoroughly utilized for present day, making, food, beautifiers and medications application regardless by a wide edge. An immense piece of those mixes are blended misleadingly and possibly cause regular and toxicology issues because of the unmanageable and suffering nature of those substances [31][32][33][34][35] . With current advances in biotechnology, thought has been paid to the decision of normal great cycles for making various kinds of biosurfactants from microorganisms [35][36][37][38][39][40] .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Statistical Approach to Optimize Production of Biosurfactant by Achromobacter Xylos

Golamari¹,

Kadiyala²,

Devi³

et al. 2022

Int J Life Sci Pharm Res

View full text Add to dashboard Cite

This study aimed to optimize medium composition biosurfactant production of achromobacter xylos using response surface quadratic model . Lipoprotein and lipopeptides are used in many industries such as petroleum refining, pharmaceutical, mining, agriculture and bioprocess industries. The point of this assessment was to pull out and portray the biosurfactant passing on restriction of microorganisms from oil corrupted soil and considering their advancement energy at various temperatures and pH. The separation and growth study was directed in MSM medium using lamp fuel oil as sole carbon hotspot for bacterial turn of events. Confined strains were found to be Gram positive bacillus and in general Gram's positive minuscule life forms can convey lipopeptides type biosurfactants. The ideal conditions for achromobacteria xylos growth were discovered to be at pH seven (7) and temperature 30oC. Central composite design (CCD) was utilized to pick the following medium components (MgSO4, NaNO3, CaCl2, (NH4)2SO4, FeSO4, and KH2PO4). Central composite arrangement (CCD) of RSM was utilized to analyze the four parts at five stages, and biosurfactant fixation was evaluated as reaction. Backslide coefficients were directed by backslide examination, and the quadratic model condition was settled. R2 an impetus for bio-surfactant was endeavored to be 0.7527, showing that the quadratic model was basic with the exploratory outcomes. Confirmation of the numerical model was driven by playing out the assessment with the normal overhauled values, and bio-surfactant production was found to be 10.53 g/L. Underwriting of the normal quadratic model was 97.3% exact with the test results facilitated under the ideal conditions. CaCl2, (NH4)2SO4, FeSO4, and KH2PO4 were perceived as successful portions for bio-surfactant delivering 98% of achromobacter xylos microorganism.

show abstract

Optimization of Cellulase ProducingAspergillus flavusSB4 by Solid State Fermentation using Response Surface Methodology (RSM)-CCD

Cited by 13 publications

References 7 publications

Expression of genes encoding cellulolytic enzymes in some Aspergillus species

Expression of genes encoding cellulolytic enzymes in some Aspergillus species

Analysis of Gut Flora From Damp Wood Termites (Trinervitermes Spp.) and Extraction, Characterization of Cellulase From the Isolate

Statistical Approach to Optimize Production of Biosurfactant by Achromobacter Xylos

Contact Info

Product

Resources

About