Optimization of Cellulase Production
from &lt;i&gt;Bacillus albus&lt;/i&gt; (MN755587) and Its
Involvement in Bioethanol Production

Abada, Emad; Elbaz, Reham M.; Sonbol, Hana; Korany, Shereen Magdy

doi:10.15244/pjoes/129697

Cited by 10 publications

(6 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with the obtained results, Abada et al . [ 11 ] reported that the maximum cellulase activity (19 IU/ml) produced by bacterial strain B. albus was attained at an inoculum size of 3% (v/v). On the other hand, cellulase enzyme was more active at an inoculum size of bacterial strain B. licheniformis 1-1v with a concentration of 1% [ 39 ].…”

Section: Resultsmentioning

confidence: 99%

“…Cellulase enzymes from thermotolerant bacteria are essential in the production of bioethanol and other biofuels, as they convert cellulose into fermentable sugars during the biomass conversion process. The enzymes' thermotolerance is particularly useful in these procedures since it accelerates and improves cellulose degradation, leading to greater biofuel yields [ 11 ]. Thermostable cellulases have uses in agricultural sectors and the feed industry.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Synthesis, Optimization, and Characterization of Cellulase Enzyme Obtained from Thermotolerant Bacillus subtilis F3: An Insight into Cotton Fabric Polishing Activity

Fouda,

Alshallash,

Atta

et al. 2023

J. Microbiol. Biotechnol.

View full text Add to dashboard Cite

The efficacy of 40 bacterial isolates obtained from hot spring water samples to produce cellulase enzymes was investigated. As a result, the strain Bacillus subtilis F3, which was identified using traditional and molecular methods, was selected as the most potent for cellulase production. Optimization was carried out using one-factor-at-a-time (OFAT) and BOX-Behnken Design to detect the best conditions for the highest cellulase activity. This was accomplished after an incubation period of 24 h at 45°C and pH 8, with an inoculum size of 1% (v/v), 5 g/l of peptone as nitrogen source, and 7.5 g/l of CMC. Moreover, the best concentration of ammonium sulfate for cellulase enzyme precipitation was 60% followed by purification using a dialysis bag and Sephadex G-100 column chromatography to collect the purified enzyme. The purified cellulase enzyme was characterized by 5.39-fold enrichment, with a specific activity of 54.20 U/mg and a molecular weight of 439 kDa. There were 15 amino acids involved in the purified cellulase, with high concentrations of 160 and 100 mg/l for glycine and proline respectively. The highest stability and activity of the purified cellulase was attained at pH 7 and 50°C in the presence of 150 ppm of CaCl 2 , NaCl, and ZnO metal ions. Finally, the biopolishing activity of the cellulase enzyme, as indicated by weight loss percentages of the cotton fabric, was dependent on concentration and treatment time. Overall, the thermotolerant B. subtilis F3 strain has the potential to provide highly stable and highly active cellulase enzyme for use in biopolishing of cotton fabrics.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synthesis, Optimization, and Characterization of Cellulase Enzyme Obtained from Thermotolerant Bacillus subtilis F3: An Insight into Cotton Fabric Polishing Activity

Fouda,

Alshallash,

Atta

et al. 2023

J. Microbiol. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Because of this, cellulose or lignocellulose can be used for the biological production of cellulase using bacterial or fungal organisms [8]. So, using bacterial or fungal enzymes, cellulose or lignocellulose can be used to produce cellulase through a biological process [9]. It is synthesized by plants and can provide energy and renewable chemicals in bio manufacturing to create a sustainable bio-economy.…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, most organic solvents and water are insoluble in it [11]. On the other hand, these traditional procedures could result in hazardous contamination of the environment [9].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Chemical Treated Spent Mushroom Substrate (SMS) on Lignocellulosic Content

Adi,

Izhar,

Ibrahim

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

A by-product of the mushroom industry, spent mushroom substrate (SMS) is primarily made of lignocellulosic agricultural waste. SMS contains cellulose (38–46.6%), lignin (25–34.5%), and hemicellulose (19–27.7%) and is nutrient-rich. The primary objectives of this study included the characterization of SMS, analysis of lignocellulosic content prior to and following pre-treatment, analysis of the effects of various NaOH concentrations with varying molarities (1.0 to 5.0 Molar), and analysis of surface morphology using a scanning electron microscope (SEM). The result that comparison between untreated and treated sample The result for untreated are contain high amount of lignin from 23.85% to 27.39% and for treated are amount reduce from 20.6% to 23.85%. The difference of NaOH concentration from 1.0 to 5.0 Molar, so the best pre-treatment is alkaline pre-treatment from 1.0 to 3.0 M are the best concentration to treat SMS sample from alkaline pre-treatment and proved sodium hydroxide pre-treatment as an effective method to reduce the hemicellulose and lignin contents. Images clearly showed how the pre-treatment could alter the biomass’s structural makeup and distort it, increasing the surface area that was open to enzymatic attack. The pre-treated SMS’s surface had numerous sporadic pores and cracks. The study’s findings demonstrated that SMS from the P. sajor-caju species has the potential to provide a new source of glucose for bioethanol production.

show abstract

“…Cellulase production has been extensively studied in the bacterial genus Bacillus . Numerous aerobic bacterial strains, including Bacillus megaterium [12], Bacillus albus [13], Bacillus subtilis [14], Bacillus cereus [15], Bacillus circulans [16], Bacillus pumilus [17], Cellulomonas uda [18], Cellulomonas flavigena [19], Cellulomonas fimi [20], and some anaerobic bacteria, including Clostridium thermocellum [21] and Fibrobacter succinogenes [22] reported to produce cellulase enzyme.…”

Section: Introductionmentioning

confidence: 99%

Purified cellulase‐mediated simultaneous sugar utilization by Bacillus albus isolated from Similipal, Odisha, India

2023

View full text Add to dashboard Cite

Among 24 isolated cellulolytic bacteria from Similipal Biosphere Reserve, the most efficient isolate was recognized as a strain of Bacillus albus. This strain of B. albus was evaluated for cellulase production and the cellulase activity was measured in submerged fermentation using substrate carboxymethyl cellulose (CMC). Different nutritional (carbon, nitrogen, and metal‐ion sources) and physical variables (pH, temperature, substrate concentration, and incubation time) during the growth of B. albus were optimized to obtain maximum cellulase activity. The highest cellulase activity of 5.79 U/mL for B. albus was observed at pH 6.75, temperature 37.5°C, CMC concentration 8.5 g/L, and 42 h incubation time. Further, supplementation of glucose as a subsidiary carbon source, yeast extract, peptone as nitrogen sources, and MgSO4 and MnSO4 as metal‐ion sources enhance the cellulase activity of B. albus. The purified enzyme was reported to have a molecular weight of ∼54 kDa as determined by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. A zymogram analysis evidenced the cellulase activity of the purified enzyme fractions obtained from diethylaminoethyl cellulose chromatography. The purified cellulase was reported to have an optimum pH and temperature of 7.0°C and 50°C, respectively with a capacity of retaining its 60% residual activity within pH 6.0–8.0 and temperature 30–40°C, respectively. The metal ions, K+ and Na+ were the activators, while Pb2+ and Hg2+ were the inhibitors for the purified cellulase. The purified cellulase showed Km and Vmax values of 0.38 M and 8.19 U/mL, respectively, in presence of the substrate CMC and also simultaneous consumption of both hexose and pentose sugars.

show abstract

Optimization of Cellulase Production from <i>Bacillus albus</i> (MN755587) and Its Involvement in Bioethanol Production

Cited by 10 publications

References 21 publications

Synthesis, Optimization, and Characterization of Cellulase Enzyme Obtained from Thermotolerant Bacillus subtilis F3: An Insight into Cotton Fabric Polishing Activity

Synthesis, Optimization, and Characterization of Cellulase Enzyme Obtained from Thermotolerant Bacillus subtilis F3: An Insight into Cotton Fabric Polishing Activity

The Effect of Chemical Treated Spent Mushroom Substrate (SMS) on Lignocellulosic Content

Purified cellulase‐mediated simultaneous sugar utilization by Bacillus albus isolated from Similipal, Odisha, India

Contact Info

Product

Resources

About