Optimization of Chitosan hydrolysis by Cellulase Enzyme to produce Chitooligosaccharide

Abstract

In order to increase the water solubility of chitosan and potential application to products that is good for human health. Chitosan was caried out the optimization of hydrolysis by cellulase to produce chitooligosaccharide. Chitosan with degree of deacetyl more than 80% and cellulase used in this study. Surface Response Method (RSM) - Central Composite Design (CCD) option is used to optimize the hydrolysis. Results of the study showed optimal values for hydrolysis such as 49 oC temperature, 5.9 pH, 0.76% substrate concentration, and 8.97 U/g enzyme concentration, 180 minutes hydrolysis time. More than 90% of the oligosaccharides produced were in the range less than 10 kDa. The research results are the premise for production of COS powder to water-soluble.

Caffeic acid‐grafted chitooligosaccharides downregulate MAPK and NF‐kB in RAW264.7 cells

“…The COS powder was created from our previous research (Bui et al., 2017). Briefly, 0.76% chitosan solution was prepared in 0.8% lactic acid solution.…”

Research antioxidant activity of chitooligosacchride by UV-VIS absorption spectrocopy

“…COSs solution was created from our previous research (Bui et al, 2017) Briefly, 0.8% chitosan solution was prepared in 0.8% lactic acid solution. A 0.8% chitosan solution was hydrolyzed by cellulase with optimum parameters as 50 o C, pH of 5.5, 7 UI cellulase and hydrolysis time of 180 minutes.…”