Optimization of conditions for extraction of acid-soluble collagen from grass carp (Ctenopharyngodon idella) by response surface methodology

Wang, Lingzhao; Yang, Bao; Du, Xiang; Yang, Yinfen; Liu, Jianlong

doi:10.1016/j.ifset.2008.03.001

Cited by 59 publications

(56 citation statements)

References 28 publications

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“…So checking the model adequacy is essential (Wang et al 2008). The model P-value of the chitosan molecular weight was <0.0001, which indicated that the model fitness was significant, since a P-value less than 0.0500 indicated that the model term was perfect.…”

Section: Model Fitting and Optimizationmentioning

confidence: 99%

Synthesis and Characterization of Low Molecular Weight Chitosan

Yuan¹,

Jiang²,

Xing³

et al. 2015

BioResources

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Chitosan can be widely used in many areas owing to its unique properties, although its poor solubility in water is still a limiting factor. In the present study, low molecular weight chitosan (LMWC) was prepared by degradation with NaClO so that chitosan was able to dissolve in water. Chitosan to liquor ratio, NaClO content, temperature, and time were considered variables of NaClO degradation, and the Box-Behnken design was used to determine optimal conditions. There was good agreement between the experimental data and their predicted counterparts. The optimum conditions for chitosan degradation were estimated to be 1:67.91 of chitosan to liquor ratio, 22.03% of NaClO content, a temperature of 90.3 °C, and a time of 3.07 h. It was found that synthesis under these optimized conditions achieved the lowest molecular weight (10,937.4 Daltons). In addition, Fourier transform infrared spectroscopy, X-ray diffractograms, and thermogravimetric analysis showed that the structure of LMWC was similar to the original chitosan, while the crystallinity and thermal stability decreased after degradation.

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Section: Model Fitting and Optimizationmentioning

confidence: 99%

Synthesis and Characterization of Low Molecular Weight Chitosan

Yuan¹,

Jiang²,

Xing³

et al. 2015

BioResources

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“…For decades, collagen has been exploited in medical applications owing to its extraordinary biocompatibility and safety, particularly the ability to form fibers with extra strength and high stability by self-aggregation in the drug delivery system [1]. It serves as the most popular precursor of gelatin, which is widely applied to commercial products [2].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the extraction of collagen from catfish aids in boosting up the commercial value of these cheaply abundant natural resources besides providing a new approach in finding alternative sources of safe collagen for industrial uses. Collagen is a thermosensitive compound and the extraction process is normally extracted under the condition that is lower than the room temperature [1,7]. It is very crucial to understand the effects of extraction conditions in order to obtain the highest yield of collagen under the most optimum operating conditions.…”

Section: Introductionmentioning

confidence: 99%

Collagen Extraction from Malaysian Cultured Catfish (Hybrid Clarias Sp.): Kinetics and Optimization of Extraction Conditions Using Response Surface Methodology

Kiew

Don

2012

ISRN Chemical Engineering

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A central composite design (CCD) was used for the experimental design and results analysis to obtain the optimal processing parameters (acetic acid concentration, liquid to solid ratio, and stirring speed) for the extraction of pepsin soluble collagen (PSC) from muscles of cultured hybrid catfish of Clarias sp. (Clarias gariepinus ×C. macrocephalus). Statistical analysis showed that the linear and quadratic terms of these three independent variables had significant effects on the yield of PSC. There was also an interaction between the ratio of liquid to solid and the stirring speed in affecting the extraction efficiency. Optimal conditions for a higher yield of PSC were an acetic acid concentration of 0.67 M, a liquid to solid ratio of 24.65 ml/g, and the stirring speed of 423.64 rpm. The verification of the optimization showed that the percentage error differences between the experimental and predicted values were in the range of 0.22-4.42%. The experimental values agreed with the predicted values, indicating an excellent fit of the model used and the success of the response surface methodology in modeling the extraction of PSC from the muscles of catfish. The experimental results were also fitted to the power law model and it was proven to be appropriate in describing the kinetics of collagen extraction process.

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“…Finally, we examined extraction solvents that contained other organic acids to determine if there was a better extraction solvent for pharmaceutical and cosmetic applications although acetic acid is the most commonly used organic solvent for collagen extraction due to its high extraction efficacy 28 . Inorganic acids such as HCl were not investigated, because of their known poor extraction efficacy 29 .…”

Section: Discussionmentioning

confidence: 99%