OPTIMIZATION OF CONDITIONS FOR RECOMBINANT DNA INJECTION INTO SPERMATOGENIC CELLS OF THE CHICKEN in vivo

Волкова, Н. А.; Волкова, Л. И.; Fomin, I.K.; Zinovieva, N. A.; Lotsmanova, N. S.

doi:10.15389/agrobiology.2012.6.56eng

Cited by 2 publications

(2 citation statements)

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“…In the end, the time depends on the strength of the electric field, the polarization magnitude of the dielectric fluid, the electric potential distribution on the surface of the liquid and on the inclination angle of the electrodes with respect to the liquid surface, rather, from the value of its cosine [20]. It is found that the electrostatic transducer whose inclination angle of electrodes is in the range 40 ° -70 ° has the best performance [21].…”

Section: New Materials Creation By Mixtures Fractionation In Cemetmentioning

confidence: 99%

New Material Creation by Phase Separation in Liquid Mixtures Induced by Non-Uniform Electric Field

Volkova¹,

Aletdinov²,

Fedosov³

2017

SSP

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The fractionation technology of liquid mixtures under the action of an externally applied inhomogeneous electric field and the device – a capacitive electromechanical energy transducer that implements this technology are considered in this article. The operational principle of a capacitive electromechanical energy transducer with liquid rotor is described. The conclusion on the possibility of applying this technology to the fractionation of oil containing emulsions is made.

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Section: New Materials Creation By Mixtures Fractionation In Cemetmentioning

confidence: 99%

New Material Creation by Phase Separation in Liquid Mixtures Induced by Non-Uniform Electric Field

Volkova¹,

Aletdinov²,

Fedosov³

2017

SSP

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show abstract

“…In transgenesis, a targeted genetic modification of male germ cells can be due to introducing the recombinant DNA directly into the testis parenchyma of adults (in vivo), or introducing the transformed donor spermatogonia into testes of a sterile recipient (ex vivo) for further obtaining progeny. In in vivo manipulation, gene constructs are introduced into testes of adult males by retroviral or lentiviral vectors which can integrate into host cell genome providing a highfrequency transformation of target cells [9,10]. At ex vivo transgenesis spermatogonia are genetically pre-transformed in vitro [11,12].…”

Section: Introductionmentioning

confidence: 99%