Optimization of cultivated strawberry micropropagation using а biogenic silica and green-tea-flavonoids-based mechanocomposite

Abstract

For the first time, organogenesis and physiological characteristics of Fragaria ananassa microclones (cvs. ‘Alpha’ and ‘Solnechnaya polyanka’) under the influence of mechanocomposite (MC) based on rice husks amorphous silica and flavonoids of green tea during the multiplication stage in in vitro conditions were studied. The addition of the MC (0.0, 2.5, 5.0 and 10.0 mg·L-1) to the Gamborg- Eveleg’s basal salt medium supplemented with 0.75 mg·L-1 6-benzylaminopurine has shown beneficial action on processes of organogenesis followed by enzymatic, photosynthetic, and hormonal activities of in vitro cultured strawberry plantlets. In both cultivars, the high frequency of proliferation (100 %) and maximum number of axillary shoots increased by 1.8–2.0 times on medium supplemented with 5.0 mg·L-1 MC. The concentrations of 2.5 and 5.0 mg·L-1 MC were optimal for obtaining plantlets with high physiological state in in vitro conditions. The results may be used for the development of production systems for a healthy planting material using biotechnological approaches and recommended for commercial strawberry micropropagation.

Effect of silicon chelates from plant raw materials on some physiological parameters in strawberry under in vitro conditions

“…Green tea is rich in the flavanol group of polyphenols as catechins which may constitute up to 30% of the dry weight [6]. In previous studies, we have shown the growth-regulating and adaptogenic activities of the mechanocomposite based on biogenic silica and green tea flavonoids at the stages of in vitro rooting and ex vitro acclimatization of F. × ananassa plantlets [7][8]. Since mechanocomposite contains silicon compounds and catechins with antioxidant properties [9][10], in this work, for the first time, the potential antioxidant protective effect, including superoxide dismutase, catalase, and peroxidase enzymes, of this environmentally friendly preparation will be analyzed under micropropagation of strawberry.…”