Optimization of Cultural Conditions for Cochliobolus heterostrophus Isolates from Infected Maize Plants from Different Agricultural Zones of Pakistan

Sehar, Shama; Perveen, Irum; Rehman, Abdul; Hameed, Amber; Ahmad, Yasmin; Ahmed, Safia

doi:10.9734/bmrj/2012/2019

Cited by 3 publications

(3 citation statements)

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“…However, larger size conidia (i.e., 10 celled conidia) were produced in pH 4.5 (Table 3 Results showed the ideal pH to culture B. setariae causing browntop millet leaf blight was pH 6.0 to pH 7.0 for maximum mycelial growth and abundant sporulation. Similarly, Naz et al (2012) [9] observed the maximum growth of H. maydis at pH 7.0 and Sinclair (1982) [10] noticed maximum sporulation at pH 6.5. Mishra and Prakash (1972) [11] obtained the the best growth of the H. catenarium at pH 6.8 and 7.2.…”

Section: Influence Of Ph On Mycelial Growth and Sporulation In Solid And Liquid Mediamentioning

confidence: 83%

Comparative evaluation of different media and pH for the culturing of Bipolaris setariae causing leaf blight in browntop millet in India

Ramesh¹,

Palanna²,

Arunkumar³

et al. 2021

J. Entomol. Zool. Stud.

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Browntop millet was newly included in the millet cropping system in India as one of the small millet. Incidence of leaf blight was observed to be severe in all millet growing regions of India and the causal organism was confirmed as B. setariae using molecular tools viz., ITS and GPDH genes. As the crop was newly included in the millet system, investigation was needed to carry out about disease causing agents their biology and culture conditions. In this study, an attempt was made to identify the ideal culturing and physiological requirements for culturing B. setariae. Results revealed that solid media such as Czapek Dox agar (CDA) and host leaf decoction agar with 2 per cent sucrose are ideal for better growth and abundant sporulation while, pH 6.0 to pH 7.0 were found to be ideal for radial mycelial growth and sporulation. These cultural and physiological conditions can be used for the future studies while working on the pathogen causing browntop millet leaf blight.

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Section: Influence Of Ph On Mycelial Growth and Sporulation In Solid And Liquid Mediamentioning

confidence: 83%

Comparative evaluation of different media and pH for the culturing of Bipolaris setariae causing leaf blight in browntop millet in India

Ramesh¹,

Palanna²,

Arunkumar³

et al. 2021

J. Entomol. Zool. Stud.

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“…Glucose was found to be the best carbon sources and showed maximum colony growth (66.40 mm). But different results were obtained by Naz et al (2012). She reported that sucrose was the most appropriate sources of carbon for obtaining maximum colony growth of the pathogen.…”

Section: Discussionmentioning

confidence: 96%

Effect of Different Media and pH on Mycelial Growth of Sclerotium oryzae Causing Sheath Rot of Rice

Ahmad

Biswas

Sultana

et al. 2019

South Asian J. Agric.

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Sclerotium oryzae causing sheath rot of rice, is one of the most important pathogen causing heavy crop losses in all the major rice growing areas of the world, including Bangladesh. The growth and reproduction of this soil borne pathogen depends on growing media and abiotic factors. Therefore, to evaluate the effect of different culture media and pH on mycelial radial growth of S. oryzae, the experiment was conducted in the Plant Pathology Laboratory of Agrotechnology Discipline, Khulna University, Khulna, Bangladesh. Performance of different media, carbon source and nitrogen source on radial mycelial growth was differed significantly. The maximum mycelial radial growth was observed on rice stem agar medium (70.40 mm) and minimum mycelial radial growth was recorded on cornmeal agar medium (50.60 mm). Considering carbon source, the maximum radial mycelial growth was found in glucose (66.40 mm) amended medium and no statistical differences were observed among sucrose, starch and Czapek dox agar containing medium. Based on nitrogen source, maximum radial mycelial growth was found in peptone medium (69.40 mm) but no statistical differences were exposed between Potassium nitrate (KNO3) and Sodium nitrate (NaNO3). pH- 6 played a vital role in radial mycelial growth (73.75 mm) of S. oryzae. South Asian J. Agric., 7(1&2): 67-72

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“…The maize plant can become infected by both asexual and sexual ascospores throughout the disease’s polycyclic life cycle [ 96 ]. The conidies are released from the maize-infected lesions under hot and humid circumstances, and they spread to nearby plantules by wind or raindrops [ 98 , 99 ]. After the conidies appear on the leaf of a healthy plant, they develop on the leaf’s tissue by the process of producing germination tubes that can enter through the leaf’s tissue or through natural openings like stomates or hydathode to cause infection [ 96 ].…”

Section: The Main Foliar Diseases Affecting Cerealsmentioning

confidence: 99%

Beneficial Microorganisms as Bioprotectants against Foliar Diseases of Cereals: A Review

Dehbi,

Achemrk,

Ezzouggari

et al. 2023

Plants

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Cereal production plays a major role in both animal and human diets throughout the world. However, cereal crops are vulnerable to attacks by fungal pathogens on the foliage, disrupting their biological cycle and photosynthesis, which can reduce yields by 15–20% or even 60%. Consumers are concerned about the excessive use of synthetic pesticides given their harmful effects on human health and the environment. As a result, the search for alternative solutions to protect crops has attracted the interest of scientists around the world. Among these solutions, biological control using beneficial microorganisms has taken on considerable importance, and several biological control agents (BCAs) have been studied, including species belonging to the genera Bacillus, Pseudomonas, Streptomyces, Trichoderma, Cladosporium, and Epicoccum, most of which include plants of growth-promoting rhizobacteria (PGPRs). Bacillus has proved to be a broad-spectrum agent against these leaf cereal diseases. Interaction between plant and beneficial agents occurs as direct mycoparasitism or hyperparasitism by a mixed pathway via the secretion of lytic enzymes, growth enzymes, and antibiotics, or by an indirect interaction involving competition for nutrients or space and the induction of host resistance (systemic acquired resistance (SAR) or induced systemic resistance (ISR) pathway). We mainly demonstrate the role of BCAs in the defense against fungal diseases of cereal leaves. To enhance a solution-based crop protection approach, it is also important to understand the mechanism of action of BCAs/molecules/plants. Research in the field of preventing cereal diseases is still ongoing.

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Optimization of Cultural Conditions for Cochliobolus heterostrophus Isolates from Infected Maize Plants from Different Agricultural Zones of Pakistan

Cited by 3 publications

References 0 publications

Comparative evaluation of different media and pH for the culturing of Bipolaris setariae causing leaf blight in browntop millet in India

Comparative evaluation of different media and pH for the culturing of Bipolaris setariae causing leaf blight in browntop millet in India

Effect of Different Media and pH on Mycelial Growth of Sclerotium oryzae Causing Sheath Rot of Rice

Beneficial Microorganisms as Bioprotectants against Foliar Diseases of Cereals: A Review

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