Optimization of culture conditions for secondary mycelial growth of wild macrofungi from selected areas in Central Luzon, Philippines

Abstract

This work highlighted the optimum culture conditions for secondary mycelia of selected Philippine wild macrofungi. The growth of different macrofungal species were evaluated on various indigenous culture media namely: coconut water gulaman, corn grit decoction gulaman, rice bran decoction gulaman, potato sucrose gulaman and potato dextrose agar (control). The effect of different physical conditions such as pH, illumination and temperature was also investigated. The daily mycelia diameter, mycelial density and incubation period were used in determining the optimum growth conditions.Among the indigenous culture media evaluated, coconut water gulaman significantly recorded the largest mycelia growth diameter in ten mushroom species/strains (mostly lignolytic) while potato sucrose gulaman favored the growth of two strains of V. volvacea. No significant difference in mycelial diameter of C. cinerea Sto Domingo strain on different indigenous culture media was recorded In terms of the pH of the media, most of the evaluated mushrooms registered maximum mycelial growth and very thick to thick mycelial density in a medium having a pH ranging from 6.0 to 7.0. Moreover, illumination significantly influenced the mycelial growth of four mushroom species. Incubation in lighted condition favored the growth of L. sajor caju, and S. commune CLSU strain whereas dark condition showed maximum growth of G. lucidum strain B and L. tigrinus CLSU strain. G. lucidum strain A, L. tigrinus CLSU strain, G. lucidum strain B and C. cinerea Sto Domingo strain incubated at room temperature (32 + 0.

Molecular identification and optimization of cultural conditions for mycelial biomass production of wild strain of Chlorophyllum molybdites (G.Mey) Massee from the Philippines

“…This suggested that this mushroom can grow in a wide range of pH. In general, mushrooms can grow in a wide range of pH of the medium [30]. Interestingly, Jayasinghe et al [35] confirmed it on his report that G. lucidum strains could grow on potato dextrose agar at a broad pH range, such as pH 5.0-9.0.…”

“…Furthermore, Sung et al [44] observed no obvious difference in the colony diameter of Ophiocordyceps longissima between light and dark conditions on solid media. In contrary, Kalaw et al [30] revealed that illumination significantly influenced the mycelial growth of five mushroom species on solid medium: L. sajor-caju and S. commune (CLSU strain), whereas dark condition showed maximum growth of G. lucidum (strain B), L. tigrinus (CLSU strain), G. lucidum (strain A), and C. cinerea (Sto. Domingo strain).…”

“…Luangharn et al [29] observed that Laetiporus sulphureus produced the largest colony diameter and mycelial density in potato dextrose agar among six culture medium tested. Kalaw et al [30] used potato sucrose gulaman on two strains of V. volvacea which showed the largest colony diameter and shortest incubation. The observation of Soytong and Asue [31] in Pleurotus giganteus suggested that although the mycelial growth produced in PDD was slower than the other media, the mycelia were thick and heavy.…”

Effects of culture media and physical factors on the mycelial growth of the three wild strains of Volvariella volvacea from Ecuador

“…It can be seen that the three strains could grow from pH of 5.5-8.0, but with optimum pH at 6.5-7.5 for La Clementina and Vinces and at 6.5-7.0 for Montalvo. Similarly, Rang-ayan and CLSU strains of V. volvacea showed favorable mycelial growth at a wide range of pH with very thick mycelia at pH 7.5-8.0 [8]. In liquid culture, the optimum pH of V. volvacea was at pH 6.0 [9].…”

“…This finding conforms with the study of Dulay et al [9] who reported that Sabouraud dextrose broth produced the highest biomass yield of Schizophyllum commune, Ganoderma lucidum, V. volvacea, and Pleurotus cystidiosus in submerged culture. However, in the evaluation of mycelial growth using indigenous culture media, the two strains of V. volvacea (Rang-ayan and CLSU) showed the maximum mycelial growth on potato sucrose gulaman (PSG) and corn grit gulaman (CGG) [8]. In addition, Ukoima et al [10] demonstrated that the highest growth of V. volvacea mycelia was observed on palm fiber culture medium.…”