The proximate composition and functionality of Lentinus tigrinus were evaluated to establish and popularize this mushroom as functional food source. The evaluation of functionality focused on the antibacterial and hypoglycemic activities of the mushroom extracts. An acute single oral dose toxicity test in mice was used for its biosafety analysis. The pileus contained higher amounts of protein (25.9%), fat (2.1%), and ash (7.4%) and a higher energetic value (142.1 kcal/100 g) than the corresponding stipe, whereas the stipe contained higher amounts of total carbohydrates (67.7%), which consist of dietary fiber (63.0%) and reducing sugar (4.7%), than the pileus. Biosafety analysis confirmed that L. tigrinus is an edible mushroom species; it was found to be toxicologically safe in imprinting control region mice. The administration of lyophilized hot water extract of the fruiting body (both 100 and 250 mg/ kg doses) to diabetic mice significantly lowered the glucose level by 26.9% in the third week, which was significantly comparable to the results of the antidiabetic agent glibenclamide, which was used as a positive control. In vitro antibacterial assay showed that the ethanolic extract of the fruiting body and the immobilized secondary mycelia had high antibacterial activities against Staphylococcus aureus but not on Escherichia coli. Combining its useful nutrients and significant biological properties, L. tigrinus can be considered a natural source of safe nutraceuticals.

Mushrooms are an important natural source of food and medicine. In the Philippines, only a few studies have been conducted on the diversity of mushrooms especially in the mountainous areas. The present study was conducted to document the species of macrofungi found in Paracelis, Mountain Province. The knowledge gained from this study can reveal their importance to the community. A total of 37 macrofungi belonging to 16 families, 26 genera, and 29 species were collected and identified. Twenty nine of the collected macrofungi were identified up to its species level and eight were only identified at its genus level. The collected samples were subjected to morphological identification based on its macroscopic and microscopic characteristics. The identified macrofungi were: Auricularia auricula-judae, Conocybe arrhenii, Coprinellus disseminatus, Coprinus sp., Crepidotus mollis, Daldinia concentrica, Earliella scabrosa, Favolus acervatus, Fomes fomentarium, Ganoderma applanatum, Ganoderma fornicatum, Ganoderma lucidum, Hygrocybe sp., Irpex lacteus, Lentinus strigosus, Lenzites elegans, Lepiota lilacea, Lepiota sp., Marasmiellus ramealis, Microporus xanthopus, Mycena sp., Panellus mitis, Paneolus cyanescens, Parasola plicalitis, Psathyrella candolleana, Psathyrella sp., Russula sp. Schizophyllum commune, Trametes elegans, Trametes gibbosa, Trametes hirsuta, Trametes versicolor, Trametes sp. 1, Trametes sp. 2, and Xylaria papulis. Out of these macrofungi, four species were identified as edible, viz: Auricularia auricula, Lentinus strigosus, Coprinus disseminatu, and Schizophyllum commune.

This paper highlights the teratogenic and toxic effects of Ganoderma lucidum (Lingzhi or Reishi mushroom) extract on zebrafish embryos. Hatchability, malformations, and lethality rate of zebrafish embryos were assessed to provide valuable information regarding the potential teratogenic activity of G. lucidum. Hatching was completed 48 h post treatment application (hpta) at 1% or lower concentrations of extract and embryo water. The hatching rate of embryos treated with 5% or higher concentrations was significantly lower (p> 0.05) than the control. Tail malformation was the most marked morphological abnormality in embryos at 72 hpta, which was obviously caused by 1% extract (55.56% tail malformation) and was observed in all embryos exposed to 5% of extract. Growth retardation was evident in embryos exposed to 5%, 10%, and 20%. However, lethal effect of extract of G. lucidum was dependent on dose and time of exposure. Mortality rates of embryos treated with 5% (44.44%) or higher concentrations of the extract was significantly higher (p > 0.05) than that of the control embryos at 72 hpta. These results suggest that G. lucidum extract has lethal and sub-lethal effects on zebrafish embryos.

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