Optimization of Culture Conditions for L-Lysine Fermentation by Corynebacterium
glutamicum

Shah, Abdul Haleem; Hameed, Abdul; Ahmad, Shoaib; Khan, Gul Majid

doi:10.3923/jbs.2002.151.156

Cited by 7 publications

(1 citation statement)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…inoculum size of 5% enhanced methionine production by B. thuringiensis EC1. High inoculum size results in too much biomass and depletes product formation [18] and also reduces dissolved oxygen, and increases competition towards nutrient [19]. Very low inoculum size may lead to insufficient biomass and reduced metabolite formation [17].…”

Section: Resultsmentioning

confidence: 99%

Optimization of Fermentation Conditions of <i>Bacillus thuringiensis</i> EC1 for Enhanced Methionine Production

Anakwenze¹,

Ezemba²,

Ekwealor³

2014

AiM

View full text Add to dashboard Cite

Bacillus thuringiensis EC1, isolated from the fermented oil bean seed, Pentachletra macrophila Benthan, produced a methionine yield of 1.89 mg/ml. The influence of cultural conditions on methionine accumulation by B. thuringiensis EC1 showed that a 20% medium/fermenter volume ratio and a 5% inoculum size increased methionine yield. The carbon of choice was maltose and at 8% level stimulated methionine production. Among the nitrogen sources studied, ammonium sulphate was found to be the best and at 1% concentration produced a methionine yield of 2.56 mg/ml. All growth-promoting substances and their mixtures enhanced methionine accumulation by B. thuringiensis EC1. The effect of Vitamins on methionine production showed that riboflavin and thiamine HCl at 1.0 µg/ml yielded 2.49 mg/ml and 2.80 mg/ml methionine respectively. The influence of bivalent metals on methionine accumulation indicated that Zn 2+ at all concentration stimulated methionine production. Mg 2+ and Ba 2+ at 0.1 µg/ml and 10.0 µg/ml respectively improved methionine yield. Optimizing the cultural conditions of B. thuringiensis EC1 in submerged medium gave a methionine yield of 3.18 mg/ml.

show abstract

Section: Resultsmentioning

confidence: 99%

Optimization of Fermentation Conditions of <i>Bacillus thuringiensis</i> EC1 for Enhanced Methionine Production

Anakwenze¹,

Ezemba²,

Ekwealor³

2014

AiM

View full text Add to dashboard Cite

show abstract

Scale‐up from shake flasks to fermenters in batch and continuous mode with Corynebacterium glutamicum on lactic acid based on oxygen transfer and pH

Seletzky

Noak

Fricke

et al. 2007

Biotech & Bioengineering

View full text Add to dashboard Cite

Scale-up from shake flasks to fermenters has been hampered by the lack of knowledge concerning the influence of operating conditions on mass transfer, hydromechanics, and power input. However, in recent years the properties of shake flasks have been described with empirical models. A practical scale-up strategy for everyday use is introduced for the scale-up of aerobic cultures from shake flasks to fermenters in batch and continuous mode. The strategy is based on empirical correlations of the volumetric mass transfer coefficient (k(L) a) and the pH. The accuracy of the empirical k(L) a correlations and the assumptions required to use these correlations for an arbitrary biological medium are discussed. To determine the optimal pH of the culture medium a simple laboratory method based on titration curves of the medium and a mechanistic pH model, which is solely based on the medium composition, is applied. The effectiveness of the scale-up strategy is demonstrated by comparing the behavior of Corynebacterium glutamicum on lactic acid in shake flasks and fermenters in batch and continuous mode. The maximum growth rate (micro(max) = 0.32 h(-1)) and the oxygen substrate coefficient (Y O2 /S= 0.0174 mol/l) of C. glutamicum on lactic acid were equal for shake flask, fermenter, batch, and continuous cultures. The biomass substrate yield was independent of the scale, but was lower in batch cultures (Y(X/S) = 0.36 g/g) than in continuous cultures (Y(X/S) = 0.45 g/g). The experimental data (biomass, respiration, pH) could be described with a simple biological model combined with a mechanistic pH model.

show abstract

Metabolic activity of Corynebacterium glutamicum grown on l-lactic acid under stress

Seletzky

Noack

Fricke

et al. 2006

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Respiration measurement in shake flasks is introduced as a new method to characterize the metabolic activity of microorganisms during and after stress exposure. The major advantage of the new method is the possibility to determine the metabolic activity independent of manual sampling without the necessity to change the culture vessel or the cultivation medium. This excludes stress factors, which may be induced by transferring the microorganisms to plates or respirometers. The negative influence, which interruptions of the shaker during sampling times may have on the growth of microorganisms was demonstrated. The applicability of the method was verified by characterizing the behavior of Corynebacterium glutamicum grown on the carbon source L: -lactic acid under stress factors such as carbon starvation, anaerobic conditions, lactic acid, osmolarity, and pH. The following conditions had no effect on the metabolic activity of C. glutamicum: a carbon starvation of up to 19 h, anaerobic conditions, lactic acid concentrations up to 10 g/l, 3-(N-morpholino) propanesulfonic acid buffer concentrations up to 42 g/l, or pH from 6.4 to 7.4. Lactic-acid concentrations from 10 to 30 g/l lead to a decrease of the growth rate and the biomass substrate yield without effecting the oxygen substrate conversion. Without adaptation, the organism did not grow at pH< or =5 or > or =9.

show abstract

Optimization of Culture Conditions for L-Lysine Fermentation by Corynebacterium glutamicum

Cited by 7 publications

References 18 publications

Optimization of Fermentation Conditions of <i>Bacillus thuringiensis</i> EC1 for Enhanced Methionine Production

Optimization of Fermentation Conditions of <i>Bacillus thuringiensis</i> EC1 for Enhanced Methionine Production

Scale‐up from shake flasks to fermenters in batch and continuous mode with Corynebacterium glutamicum on lactic acid based on oxygen transfer and pH

Metabolic activity of Corynebacterium glutamicum grown on l-lactic acid under stress

Contact Info

Product

Resources

About

Optimization of Culture Conditions for L-Lysine Fermentation by Corynebacterium glutamicum

Cited by 7 publications

References 18 publications

Optimization of Fermentation Conditions of &lt;i&gt;Bacillus thuringiensis&lt;/i&gt; EC1 for Enhanced Methionine Production

Optimization of Fermentation Conditions of &lt;i&gt;Bacillus thuringiensis&lt;/i&gt; EC1 for Enhanced Methionine Production

Scale‐up from shake flasks to fermenters in batch and continuous mode with Corynebacterium glutamicum on lactic acid based on oxygen transfer and pH

Metabolic activity of Corynebacterium glutamicum grown on l-lactic acid under stress

Contact Info

Product

Resources

About

Optimization of Fermentation Conditions of <i>Bacillus thuringiensis</i> EC1 for Enhanced Methionine Production

Optimization of Fermentation Conditions of <i>Bacillus thuringiensis</i> EC1 for Enhanced Methionine Production